Serum Albumin/ALB Protein, Rabbit (HEK293, His)

ALB Protein is a serum protein synthesized by the liver that plays a crucial role in maintaining fluid balance and transporting various molecules in the bloodstream. It is involved in the regulation of osmotic pressure and acts as a carrier for hormones, fatty acids, and drugs. Dysregulation of ALB Protein has been associated with various diseases, including liver disease and kidney disease. Targeting ALB Protein may offer potential therapeutic interventions in these conditions by restoring fluid balance, improving transport functions, and supporting liver and kidney function. Serum Albumin/ALB Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived Serum Albumin/ALB protein, expressed by HEK293, with C-His labeled tag. The total length of Serum Albumin/ALB Protein, Rabbit (HEK293, His) is 584 a.a., with molecular weight of 60-70 kDa.

ALB Protein, a versatile molecule, binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin, and drugs. Its primary role is to regulate the colloidal osmotic pressure of blood. Acting as a major zinc transporter in plasma, it binds around 80% of all plasma zinc, while also functioning as a major calcium and magnesium transporter, binding approximately 45% of circulating calcium and magnesium in plasma. ALB Protein may possess multiple calcium-binding sites and can potentially bind calcium in a non-specific manner. The interaction between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood. Its affinity follows the rank order of zinc > calcium > magnesium. Furthermore, ALB Protein interacts with the bacterial siderophore enterobactin, inhibiting its iron uptake and potentially limiting the growth of enteric bacteria such as E.coli. Notably, it does not hinder iron uptake by the bacterial siderophore aerobactin. ALB Protein also plays a role in ALB homeostasis through its interaction with FCGRT, and it interacts with TASOR as well. In plasma, ALB Protein forms a covalently-linked complex with chromophore-bound alpha-1-microglobulin, without impeding the binding of fatty acids to ALB.

Rabbit

HEK293

C-His

P49065-1 (E25-G608)

100009195  [NCBI]

60-70 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.