Serum Albumin/ALB Protein, Rat (HEK293, His)

Serum Albumin/ALB Protein, Rat (HEK293, His) is the recombinant rat-derived Serum Albumin protein, expressed by HEK293, with N-His tag.

Serum Albumin is a multifunctional plasma protein primarily involved in regulating the colloidal osmotic pressure of blood. It binds water, Ca²⁺, Na(+), K(+), fatty acids, hormones, bilirubin, and drugs. As the major zinc transporter in plasma, it typically binds about 80% of all plasma zinc (By similarity). It also serves as the major calcium and magnesium transporter, binding approximately 45% of circulating calcium and magnesium (By similarity). The shared zinc/calcium-binding site at residue Asp-273 suggests crosstalk between zinc and calcium transport in blood (By similarity), with affinity order zinc > calcium > magnesium (By similarity). Additionally, it binds the bacterial siderophore enterobactin, inhibiting enterobactin-mediated iron uptake by E.coli from ferric transferrin, potentially limiting iron utilization and growth of enteric bacteria (By similarity), though it does not block iron uptake via aerobactin (By similarity).

Rat

HEK293

N-8*His

P02770 (E25-A608)

24186

Full Length of Mature Protein

EAHKSEIAHRFKDLGEQHFKGLVLIAFSQYLQKCPYEEHIKLVQEVTDFAKTCVADENAENCDKSIHTLFGDKLCAIPKLRDNYGELADCCAKQEPERNECFLQHKDDNPNLPPFQRPEAEAMCTSFQENPTSFLGHYLHEVARRHPYFYAPELLYYAEKYNEVLTQCCTESDKAACLTPKLDAVKEKALVAAVRQRMKCSSMQRFGERAFKAWAVARMSQRFPNAEFAEITKLATDVTKINKECCHGDLLECADDRAELAKYMCENQATISSKLQACCDKPVLQKSQCLAEIEHDNIPADLPSIAADFVEDKEVCKNYAEAKDVFLGTFLYEYSRRHPDYSVSLLLRLAKKYEATLEKCCAEGDPPACYGTVLAEFQPLVEEPKNLVKTNCELYEKLGEYGFQNAVLVRYTQKAPQVSTPTLVEAARNLGRVGTKCCTLPEAQRLPCVEDYLSAILNRLCVLHEKTPVSEKVTKCCSGSLVERRPCFSALTVDETYVPKEFKAETFTFHSDICTLPDKEKQIKKQTALAELVKHKPKATEDQLKTVMGDFAQFVDKCCKAADKDNCFATEGPNLVARSKEALA

Predicted band size: 67.52 kDa; Observed band size: 65-70 kDa.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.