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  5. Siglec-3/CD33 Protein, Cynomolgus/Rhesus Macaque (Biotinylated, HEK293, His)

Siglec-3/CD33 Protein, Cynomolgus/Rhesus Macaque (Biotinylated, HEK293, His)

Cat. No.: HY-P75652
Handling Instructions

Siglec-3/CD33, a sialic-acid-binding lectin, crucially mediates cell-cell interactions and immune cell quiescence. Preferring sialic acid on specific mucins, it forms homodimers, interacting with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. CD33 also engages C1QA through its C-terminus, activating CD33 inhibitory motifs. Siglec-3/CD33 Protein, Cynomolgus/Rhesus Macaque (Biotinylated, HEK293, His) is the recombinant Rhesus Macaque-derived Siglec-3/CD33 protein, expressed by HEK293 , with C-His labeled tag. The total length of Siglec-3/CD33 Protein, Cynomolgus/Rhesus Macaque (Biotinylated, HEK293, His) is 248 a.a., with molecular weight of ~27.3 kDa.

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Description

Siglec-3/CD33, a sialic-acid-binding lectin, crucially mediates cell-cell interactions and immune cell quiescence. Preferring sialic acid on specific mucins, it forms homodimers, interacting with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. CD33 also engages C1QA through its C-terminus, activating CD33 inhibitory motifs. Siglec-3/CD33 Protein, Cynomolgus/Rhesus Macaque (Biotinylated, HEK293, His) is the recombinant Rhesus Macaque-derived Siglec-3/CD33 protein, expressed by HEK293 , with C-His labeled tag. The total length of Siglec-3/CD33 Protein, Cynomolgus/Rhesus Macaque (Biotinylated, HEK293, His) is 248 a.a., with molecular weight of ~27.3 kDa.

Background

Siglec-3/CD33, a sialic-acid-binding immunoglobulin-like lectin, plays a crucial role in mediating cell-cell interactions and maintaining immune cells in a resting state. It exhibits a preference for binding sialic acid on the short O-linked glycans of specific mucins. The protein forms homodimers through disulfide linkages and interacts with signaling molecules such as PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. Additionally, CD33 engages with C1QA via its C-terminus, leading to the activation of CD33 inhibitory motifs.

Species

Rhesus Macaque

Source

HEK293

Tag

C-His

Accession

A0A2K5W2R5 (M1-G248)

Gene ID

102117580  [NCBI]

Molecular Construction
N-term
CD33 (M1-G248)
Accession # A0A2K5W2R5
His
C-term
Synonyms
Myeloid Cell Surface Antigen CD33; Siglec-3; gp67; CD33; SIGLEC3
Molecular Weight

Approximately 27.3 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Siglec-3/CD33 Protein, Cynomolgus/Rhesus Macaque (Biotinylated, HEK293, His)
Cat. No.:
HY-P75652
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