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Osteopontin/OPN Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P701071
COA Handling Instructions

Osteopontin/OPN Protein, a vital member of the osteopontin family, plays a pivotal role in cell adhesion, immune response modulation, and tissue mineralization. Sharing conserved features within the family, Osteopontin's versatile nature contributes to both homeostatic and pathological conditions. Its study provides valuable insights into the broader understanding of the osteopontin family's roles in biological functions, offering potential therapeutic avenues and further exploration of intricate interactions within cellular pathways. Osteopontin/OPN Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Osteopontin/OPN protein, expressed by HEK293, with C-His labeled tag. The total length of Osteopontin/OPN Protein, Cynomolgus (HEK293, His) is 284 a.a., with molecular weight of 45-55 kDa.

For research use only. We do not sell to patients.

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Description

Osteopontin/OPN Protein, a vital member of the osteopontin family, plays a pivotal role in cell adhesion, immune response modulation, and tissue mineralization. Sharing conserved features within the family, Osteopontin's versatile nature contributes to both homeostatic and pathological conditions. Its study provides valuable insights into the broader understanding of the osteopontin family's roles in biological functions, offering potential therapeutic avenues and further exploration of intricate interactions within cellular pathways. Osteopontin/OPN Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Osteopontin/OPN protein, expressed by HEK293, with C-His labeled tag. The total length of Osteopontin/OPN Protein, Cynomolgus (HEK293, His) is 284 a.a., with molecular weight of 45-55 kDa.

Background

The Osteopontin/OPN Protein is a vital member of the osteopontin family, playing a pivotal role in various physiological processes, including cell adhesion, immune response modulation, and tissue mineralization. As part of this family, Osteopontin shares conserved features and characteristics that underscore its functional significance. Its involvement in diverse cellular activities suggests its versatile nature, contributing to both homeostatic and pathological conditions. The study of Osteopontin provides valuable insights into the broader understanding of the osteopontin family's roles in biological functions, offering potential avenues for therapeutic interventions and further exploration of its intricate interactions within cellular pathways.

Species

Cynomolgus

Source

HEK293

Tag

C-His

Accession

A0A2K5WL90 (L17-N300)

Gene ID

/

Molecular Construction
N-term
OPN (L17-N300)
Accession # A0A2K5WL90
His
C-term
Synonyms
Secreted phosphoprotein 1; Osteopontin; Bone sialoprotein 1; Nephropontin; SPP-1; BNSP; OPN; SPP1; Uropontin; BSP-1; ETA-1; BSPI
Molecular Weight

45-55 kDa

Purity

Greater than 95% as determined by reducing Tris-Bis PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Osteopontin/OPN Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Osteopontin/OPN Protein, Cynomolgus (HEK293, His)
Cat. No.:
HY-P701071
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