TFF1 Protein, Mouse (HEK293, His)

TFF1 protein functions as a mucous gel stabilizer, vital for fortifying the gastrointestinal mucosa against noxious agents. It contributes to the mucous layer's integrity, providing a crucial physical barrier for the gastrointestinal tract, safeguarding it from potential harm. TFF1's stabilizing role emphasizes its significance in preserving the mucosal barrier, essential for overall gastrointestinal health and protection. TFF1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TFF1 protein, expressed by HEK293 , with C-6*His labeled tag.

Trefoil Factor 1 (TFF1) protein acts as a stabilizer for the mucous gel that overlays the gastrointestinal mucosa, playing a crucial role in providing a robust physical barrier against a variety of noxious agents. By contributing to the integrity of the mucous layer, TFF1 aids in the protection and defense of the gastrointestinal tract from potential harmful substances. Its stabilizing function underscores its importance in maintaining the mucosal barrier, which is essential for the overall health and protection of the gastrointestinal mucosa.

Measured in inhibiting cell proliferation assay using AGS human gastric adenocarcinoma cells. The ED50 for this effect is 9.665 μg/mL, corresponding to a specific activity is 1.707×10^2 units/mg.

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  Measured in inhibiting cell proliferation assay using AGS human gastric adenocarcinoma cells. The ED₅₀ for this effect is 9.665 μg/mL, corresponding to a specific activity is 1.707×10² units/mg.

Mouse

HEK293

C-6*His

Q08423 (Q22-F87)

21784  [NCBI]

Full Length of Mature Protein

QAQAQAQAQEETCIMAPRERINCGFPGVTAQQCTERGCCFDDSVRGFPWCFHPMAIENTQEEECPF

Approximately 15 kDa, based on SDS-PAGE under reducing conditions.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.