TNF RII/TNFRSF1B Protein, Human (Biotinylated, HEK293, His-Avi)

The TNFRII protein is a receptor with high affinity for TNFSF2/TNF-α and low affinity for TNFSF1/lymphotoxin-α, and plays a key role in mediating the metabolic effects of TNF-α. The TRAF1/TRAF2 complex recruits BIRC2 and BIRC3 to TNFRSF1B/TNFR2 to regulate the apoptotic pathway. TNF RII/TNFRSF1B Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived TNF RII/TNFRSF1B protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

The TNFRII protein serves as a receptor with a high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2, thereby modulating apoptotic pathways. This receptor plays a pivotal role in mediating the majority of the metabolic effects induced by TNF-alpha. Notably, its isoform 2 exhibits the ability to block TNF-alpha-induced apoptosis, suggesting a regulatory role in antagonizing the biological activity of TNF-alpha. Furthermore, TNFRII binds to TRAF2, highlighting its involvement in downstream signaling cascades. Additionally, interactions with BMX and the activated form of XPNPEP3 further contribute to the complexity of its molecular associations.

Immobilized Human TNF-alpha His at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNFR2 His-Avi with a linear range of 0.2-5 ng/mL.

Human

HEK293

C-Avi;C-His

P20333-1 (L23-D257)

7133  [NCBI]

Extracellular Domain

Biotin

LPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPPAEGSTGD

Approximately 40-55 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4 . Normally trehalose is added as protectant before lyophilization.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.