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  4. TPO/Thyroid peroxidase Protein, Human (A257S, T725P, sf9, His)

TPO/Thyroid peroxidase Protein, Human (A257S, T725P, sf9, His)

Cat. No.: HY-P73582
Handling Instructions

Thyroid peroxidase (TPO) plays a pivotal role in iodination and coupling of tyrosines in thyroglobulin, crucial for synthesizing essential thyroid hormones T(3) and T(4). TPO/Thyroid peroxidase Protein, Human (A257S, T725P, sf9, His) is the recombinant human-derived TPO/Thyroid peroxidase protein, expressed by Sf9 insect cells , with C-10*His labeled tag. The total length of TPO/Thyroid peroxidase Protein, Human (A257S, T725P, sf9, His) is 832 a.a., with molecular weight of 90-100 kDa.

For research use only. We do not sell to patients.

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Description

Thyroid peroxidase (TPO) plays a pivotal role in iodination and coupling of tyrosines in thyroglobulin, crucial for synthesizing essential thyroid hormones T(3) and T(4). TPO/Thyroid peroxidase Protein, Human (A257S, T725P, sf9, His) is the recombinant human-derived TPO/Thyroid peroxidase protein, expressed by Sf9 insect cells , with C-10*His labeled tag. The total length of TPO/Thyroid peroxidase Protein, Human (A257S, T725P, sf9, His) is 832 a.a., with molecular weight of 90-100 kDa.
Background

Thyroid peroxidase (TPO) orchestrates the crucial processes of iodination and coupling of hormonogenic tyrosines within thyroglobulin, culminating in the production of the essential thyroid hormones T(3) and T(4).
Species

Human
Source

Sf9 insect cells
Tag

C-10*His
Accession

P07202-1 (C15-R846, A257S, T725P)
Gene ID
Molecular Construction
N-term
TPO (C15-R846, A257S, T725P)
Accession # P07202-1
10*His
C-term
Synonyms
Thyroid peroxidase; TPO; THPO
AA Sequence

CTEAFFPFISRGKELLWGKPEESRVSSVLEESKRLVDTAMYATMQRNLKKRGILSPAQLLSFSKLPEPTSGVIARAAEIMETSIQAMKRKVNLKTQQSQHPTDALSEDLLSIIANMSGCLPYMLPPKCPNTCLANKYRPITGACNNRDHPRWGASNTALARWLPPVYEDGFSQPRGWNPGFLYNGFPLPPVREVTRHVIQVSNEVVTDDDRYSDLLMAWGQYIDHDIAFTPQSTSKAAFGGGSDCQMTCENQNPCFPIQLPEEARPAAGTACLPFYRSSAACGTGDQGALFGNLSTANPRQQMNGLTSFLDASTVYGSSPALERQLRNWTSAEGLLRVHARLRDSGRAYLPFVPPRAPAACAPEPGIPGETRGPCFLAGDGRASEVPSLTALHTLWLREHNRLAAALKALNAHWSADAVYQEARKVVGALHQIITLRDYIPRILGPEAFQQYVGPYEGYDSTANPTVSNVFSTAAFRFGHATIHPLVRRLDASFQEHPDLPGLWLHQAFFSPWTLLRGGGLDPLIRGLLARPAKLQVQDQLMNEELTERLFVLSNSSTLDLASINLQRGRDHGLPGYNEWREFCGLPRLETPADLSTAIASRSVADKILDLYKHPDNIDVWLGGLAENFLPRARTGPLFACLIGKQMKALRDGDWFWWENSHVFTDAQRRELEKHSLSRVICDNTGLTRVPMDAFQVGKFPEDFESCDSIPGMNLEAWRETFPQDDKCGFPESVENGDFVHCEESGRRVLVYSCRHGYELQGREQLTCTQEGWDFQPPLCKDVNECADGAHPPCHASARCRNTKGGFQCLCADPYELGDDGRTCVDSGRLPR

Molecular Weight

90-100 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

TPO/Thyroid peroxidase Protein, Human (A257S, T725P, sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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