TSHR Protein, Human (sf9, His)

TSHR, a membrane protein, is a key regulator of thyroid cell metabolism. As a receptor for thyrothropin and thyrostimulin, its activity involves adenylate cyclase. Gene defects contribute to various hyperthyroidism types. The gene exhibits restricted expression in the thyroid (RPKM 104.1). TSHR Protein, Human (sf9, His) is the recombinant human-derived TSHR protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of TSHR Protein, Human (sf9, His) is 393 a.a., with molecular weight (affected by relative charge) of ~60 KDa.

TSHR Protein, encoded by this gene, functions as a crucial membrane protein and serves as a primary regulator of thyroid cell metabolism. Operating as a receptor for thyrotropin and thyrostimulin, its activity is mediated through adenylate cyclase. Genetic defects in this gene are associated with various forms of hyperthyroidism, highlighting its pivotal role in maintaining thyroid function. Three transcript variants encoding different isoforms have been identified. Furthermore, the expression of TSHR is notably restricted to the thyroid, with a particularly high level observed (RPKM 104.1), emphasizing its specialization in thyroid tissue and its central position in the intricate regulatory mechanisms governing thyroid metabolism.

Human

Sf9 insect cells

C-His

P16473-1/NP_000360.2 (G21-G413)

7253  [NCBI]

Approximately 56.6 KDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50mM Tris, 150mM NaCl, 10% Glycerol, 1mM TCEP, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.