TSHR Protein, Human (sf9, His, solution)

TSHR, a membrane protein, is a key regulator of thyroid cell metabolism. As a receptor for thyrothropin and thyrostimulin, its activity involves adenylate cyclase. Gene defects contribute to various hyperthyroidism types. The gene exhibits restricted expression in the thyroid (RPKM 104.1). TSHR Protein, Human (sf9, His, solution) is the recombinant human-derived TSHR protein, expressed by Sf9 insect cells , with C-His labeled tag.

TSHR Protein, encoded by this gene, functions as a crucial membrane protein and serves as a primary regulator of thyroid cell metabolism. Operating as a receptor for thyrotropin and thyrostimulin, its activity is mediated through adenylate cyclase. Genetic defects in this gene are associated with various forms of hyperthyroidism, highlighting its pivotal role in maintaining thyroid function. Three transcript variants encoding different isoforms have been identified. Furthermore, the expression of TSHR is notably restricted to the thyroid, with a particularly high level observed (RPKM 104.1), emphasizing its specialization in thyroid tissue and its central position in the intricate regulatory mechanisms governing thyroid metabolism.

Label Recombinant Human TSH alpha & beta Heterodimer Protein (His Tag) with biotin. Immobilized Recombinant Human TSH Receptor/TSHR (His Tag) at 2 μg/mL (100 μL/well) can bind biotinylated Recombinant Human TSH alpha & beta Heterodimer Protein, the EC₅₀ is 90-450 ng/mL.

Human

Sf9 insect cells

C-His

P16473-1/NP_000360.2 (G21-G413)

7253

Extracellular Domain

GMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSMQSLRQRKSVNALNSPLHQEYEENLGDSIVGYKEKSKFQDTHNNAHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTICGDSEDMVCTPKSDEFNPCEDIMG

Approximately 57 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 85%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, 10% Glycerol, 1mM TCEP, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.