TSHR Protein, Human (sf9, His, solution)
Based on 1 Customer Validation
TSHR, a membrane protein, is a key regulator of thyroid cell metabolism. As a receptor for thyrothropin and thyrostimulin, its activity involves adenylate cyclase. Gene defects contribute to various hyperthyroidism types. The gene exhibits restricted expression in the thyroid (RPKM 104.1). TSHR Protein, Human (sf9, His, solution) is the recombinant human-derived TSHR protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
TSHR, a membrane protein, is a key regulator of thyroid cell metabolism. As a receptor for thyrothropin and thyrostimulin, its activity involves adenylate cyclase. Gene defects contribute to various hyperthyroidism types. The gene exhibits restricted expression in the thyroid (RPKM 104.1). TSHR Protein, Human (sf9, His, solution) is the recombinant human-derived TSHR protein, expressed by Sf9 insect cells , with C-His labeled tag.
TSHR Protein, encoded by this gene, functions as a crucial membrane protein and serves as a primary regulator of thyroid cell metabolism. Operating as a receptor for thyrotropin and thyrostimulin, its activity is mediated through adenylate cyclase. Genetic defects in this gene are associated with various forms of hyperthyroidism, highlighting its pivotal role in maintaining thyroid function. Three transcript variants encoding different isoforms have been identified. Furthermore, the expression of TSHR is notably restricted to the thyroid, with a particularly high level observed (RPKM 104.1), emphasizing its specialization in thyroid tissue and its central position in the intricate regulatory mechanisms governing thyroid metabolism.
Label Recombinant Human TSH alpha & beta Heterodimer Protein (His Tag) with biotin. Immobilized Recombinant Human TSH Receptor/TSHR (His Tag) at 2 μg/mL (100 μL/well) can bind biotinylated Recombinant Human TSH alpha & beta Heterodimer Protein, the EC50 is 90-450 ng/mL.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag C-His
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Accession
P16473-1/NP_000360.2 (G21-G413)
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Gene ID7253
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Molecular Construction
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N-term
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TSHR (G21-G413)
Accession # NP_000360.2 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
TSHR; LGR3; Thyrotropin receptor; Thyroid-stimulating hormone receptor; TSH-R; CHNG1; HTSHR-I; TSHR Protein; Thyrotropin Receptor-I, HTSHR-I; Seven Transmembrane Helix Receptor; TSH Receptor; Thyroid Stimulating Hormone Receptor
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AA Sequence
GMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSMQSLRQRKSVNALNSPLHQEYEENLGDSIVGYKEKSKFQDTHNNAHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTICGDSEDMVCTPKSDEFNPCEDIMG
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Predicted Molecular Mass
46.1 kDa
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Molecular Weight
Approximately 57 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, 10% Glycerol, 1mM TCEP, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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