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  3. TSLP Protein, Human (Biotinylated, R127A, R130A, HEK293, His-Avi)

TSLP Protein, Human (Biotinylated, R127A, R130A, HEK293, His-Avi)

Cat. No.: HY-P78225
COA Handling Instructions

TSLP Protein, a cytokine, stimulates T-cell-attracting chemokine release from monocytes and enhances CD11c(+) dendritic cell maturation. It directly activates mast cells, possibly inducing allergic inflammation. TSLP may also possess antimicrobial properties in the oral cavity and on the skin. TSLP Protein, Human (Biotinylated, R127A, R130A, HEK293, His-Avi) is the recombinant human-derived TSLP protein, expressed by HEK293 , with C-Avi, C-His labeled tag and R127A, R130A mutation. The total length of TSLP Protein, Human (Biotinylated, R127A, R130A, HEK293, His-Avi) is 131 a.a., with molecular weight of 25-38 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

TSLP Protein, a cytokine, stimulates T-cell-attracting chemokine release from monocytes and enhances CD11c(+) dendritic cell maturation. It directly activates mast cells, possibly inducing allergic inflammation. TSLP may also possess antimicrobial properties in the oral cavity and on the skin. TSLP Protein, Human (Biotinylated, R127A, R130A, HEK293, His-Avi) is the recombinant human-derived TSLP protein, expressed by HEK293 , with C-Avi, C-His labeled tag and R127A, R130A mutation. The total length of TSLP Protein, Human (Biotinylated, R127A, R130A, HEK293, His-Avi) is 131 a.a., with molecular weight of 25-38 kDa.

Background

The TSLP protein is a cytokine that stimulates the release of T-cell-attracting chemokines from monocytes, with a particular effect on enhancing the maturation of CD11c(+) dendritic cells. Additionally, this protein can directly activate mast cells, potentially leading to the induction of allergic inflammation. It is also suggested that TSLP may have antimicrobial properties in the oral cavity and on the skin.

Biological Activity

Measured by its binding ability in a functional ELISA. When immobilized Anti-TSLP Antibody, hFc Tag at 0.5μg/ml (100μl/Well), can bind Biotinylated Human TSLP (R127A,R130A),His Tag and the EC50 is <5 ng/ml.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

Q969D9 (Y29-Q159, R127A, R130A)

Gene ID
Molecular Construction
N-term
TSLP (Y29-Q159, R127A, R130A)
Accession # Q969D9
His-Avi
C-term
Synonyms
Thymic stromal lymphopoietin; TSLP
Molecular Weight

25-38 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

TSLP Protein, Human (Biotinylated, R127A, R130A, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TSLP Protein, Human (Biotinylated, R127A, R130A, HEK293, His-Avi)
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HY-P78225
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