Tubby Protein, Human (His)

Tubby, implicated in G protein-coupled receptor signaling, binds phosphatidylinositol 4,5-bisphosphate on membranes and may play a role in nuclear transcription regulation. Potentially involved in hypothalamic weight regulation and contributes to phagocytosis of apoptotic cells. Interacts with GNAQ and TULP1. Tubby Protein, Human (His) is the recombinant human-derived Tubby protein, expressed by E. coli , with N-His labeled tag.

Tubby protein operates in signal transduction pathways initiated by heterotrimeric G protein-coupled receptors, demonstrating an affinity for membranes containing phosphatidylinositol 4,5-bisphosphate. Additionally, Tubby protein exhibits DNA-binding capability in vitro and may play a role in transcriptional regulation within the nucleus. Implications for its involvement in the hypothalamic control of body weight are suggested. Furthermore, Tubby protein contributes to the stimulation of phagocytosis, particularly in apoptotic retinal pigment epithelium (RPE) cells and macrophages. Its interactions with GNAQ and TULP1 underscore its multifaceted role in cellular processes.

Human

E. coli

N-8*His

P50607-1 (M1-E506)

/

Full Length of Isoform-1

MTSKPHSDWIPYSVLDDEGRNLRQQKLDRQRALLEQKQKKKRQEPLMVQANADGRPRSRRARQSEEQAPLVESYLSSSGSTSYQVQEADSLASVQLGATRPTAPASAKRTKAAATAGGQGGAARKEKKGKHKGTSGPAALAEDKSEAQGPVQILTVGQSDHAQDAGETAAGGGERPSGQDLRATMQRKGISSSMSFDEDEEDEEENSSSSSQLNSNTRPSSATSRKSVREAASAPSPTAPEQPVDVEVQDLEEFALRPAPQGITIKCRITRDKKGMDRGMYPTYFLHLDREDGKKVFLLAGRKRKKSKTSNYLISVDPTDLSRGGDSYIGKLRSNLMGTKFTVYDNGVNPQKASSSTLESGTLRQELAAVCYETNVLGFKGPRKMSVIVPGMNMVHERVSIRPRNEHETLLARWQNKNTESIIELQNKTPVWNDDTQSYVLNFHGRVTQASVKNFQIIHGNDPDYIVMQFGRVAEDVFTMDYNYPLCALQAFAIALSSFDSKLACE

Approximately 57 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22μm filtered solution of 20mM Tris, 300mM NaCl, 10% Glycerol, 1mM DTT, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.