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  4. Tubulin Beta Protein, Human (His)

Tubulin Beta Protein, Human (His) is the recombinant human-derived Tubulin Beta, expressed by E. coli , with C-6*His tag.

For research use only. We do not sell to patients.

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Description

Tubulin Beta Protein, Human (His) is the recombinant human-derived Tubulin Beta, expressed by E. coli , with C-6*His tag.
Background

Beta Tubulin is the major constituent of microtubules, which are cylindrical structures formed by lateral association of linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow through the addition of GTP-tubulin dimers to their ends, forming a stabilizing cap. Below this cap, tubulin dimers exist in a GDP-bound state due to the GTPase activity of alpha-tubulin.
Species

Human
Source

E. coli
Tag

C-6*His
Accession

P07437 (M1-A444)
Gene ID

203068
Molecular Construction
N-term
TUBB (M1-A444)
Accession # P07437
6*His
C-term
Protein Length

Full Length
Synonyms
TUBB5; TUBB
AA Sequence

MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA

Predicted Molecular Mass
50.6 kDa
Purity

≥ 85%, as determined by reducing SDS-PAGE.
Documentation
Handling Instructions (2659 KB)

Tubulin Beta Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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