Tyrosine Hydroxylase Protein, Mouse (sf9, His)

The Tyrosine Hydroxylase protein serves as a crucial catalyst in the biosynthesis of catecholamines, including dopamine, noradrenaline, and adrenaline, overseeing the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa)—a pivotal and rate-limiting step in this biochemical pathway. Through the utilization of tetrahydrobiopterin and molecular oxygen, Tyrosine Hydroxylase efficiently transforms tyrosine into L-Dopa, showcasing its central role in the production of essential neurotransmitters. Beyond its primary function, the enzyme exhibits versatility by also catalyzing the hydroxylation of phenylalanine and tryptophan, albeit with lower specificity. Intriguingly, Tyrosine Hydroxylase extends its influence to developmental processes, positively regulating the regression of retinal hyaloid vessels during postnatal development, highlighting its multifaceted engagement in both neurotransmitter synthesis and physiological development. Tyrosine Hydroxylase Protein, Mouse (sf9, His) is the recombinant mouse-derived Tyrosine Hydroxylase protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Tyrosine Hydroxylase Protein, Mouse (sf9, His) is 497 a.a., with molecular weight of ~65 kDa.

The Tyrosine Hydroxylase protein serves as a crucial catalyst in the biosynthesis of catecholamines, including dopamine, noradrenaline, and adrenaline, overseeing the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa)—a pivotal and rate-limiting step in this biochemical pathway. Through the utilization of tetrahydrobiopterin and molecular oxygen, Tyrosine Hydroxylase efficiently transforms tyrosine into L-Dopa, showcasing its central role in the production of essential neurotransmitters. Beyond its primary function, the enzyme exhibits versatility by also catalyzing the hydroxylation of phenylalanine and tryptophan, albeit with lower specificity. Intriguingly, Tyrosine Hydroxylase extends its influence to developmental processes, positively regulating the regression of retinal hyaloid vessels during postnatal development, highlighting its multifaceted engagement in both neurotransmitter synthesis and physiological development.

Mouse

Sf9 insect cells

N-His

P24529 (P2-S498)

21823  [NCBI]

Approximately 65 kDa

Greater than 85% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.