1. Recombinant Proteins
  2. Ubiquitin Related Proteins
  3. Ubiquitin/UBLs
  4. Ubiquitin B (UBB)
  5. UBB Protein, Human

UBB Protein, Human

Cat. No.: HY-P71101
COA Handling Instructions

UBB proteins are central regulators of the formation of covalently linked isopeptide bonds in different states: monoubiquitin, polyubiquitin chains, or linear polyubiquitin. UBB Protein, Human is the recombinant human-derived UBB protein, expressed by E. coli , with tag free. The total length of UBB Protein, Human is 76 a.a., with molecular weight of ~7.0 kDa.

For research use only. We do not sell to patients.

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Description

UBB proteins are central regulators of the formation of covalently linked isopeptide bonds in different states: monoubiquitin, polyubiquitin chains, or linear polyubiquitin. UBB Protein, Human is the recombinant human-derived UBB protein, expressed by E. coli , with tag free. The total length of UBB Protein, Human is 76 a.a., with molecular weight of ~7.0 kDa.

Background

UBB protein, a pivotal player in cellular regulation, exists either covalently attached to other proteins or in a free, unanchored state. In its covalently bound forms, UBB conjugates to target proteins through isopeptide bonds, presenting as monoubiquitin, polyubiquitin chains linked via different Lys residues, or linear polyubiquitin chains initiated at the Met residue. The functions of polyubiquitin chains are diverse and dependent on the specific Lys residue involved: Lys-6-linked ubiquitin may contribute to DNA repair, Lys-11-linked is implicated in endoplasmic reticulum-associated degradation (ERAD) and cell-cycle regulation, Lys-29-linked is associated with proteotoxic stress response and cell cycle, Lys-33-linked is engaged in kinase modification, Lys-48-linked is crucial for protein degradation via the proteasome, and Lys-63-linked plays roles in endocytosis, DNA-damage responses, and NF-kappa-B activation. Linear polyubiquitin chains, initiated at Met, are linked to cell signaling. While UBB typically conjugates to Lys residues, rare instances involve conjugation to Cys or Ser residues. In its unanchored state, free polyubiquitin contributes to distinct roles, such as activating protein kinases and participating in signaling processes. Furthermore, UBB interacts with SKP1-KMD2A and SKP1-KMD2B complexes, emphasizing its involvement in specific cellular pathways and protein interactions.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

P0CG47 (M153-G228)

Gene ID
Molecular Construction
N-term
UBB (M153-G228)
Accession # P0CG47
C-term
Synonyms
Polyubiquitin-B; UBB
AA Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

Molecular Weight

Approximately 7.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

UBB Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
UBB Protein, Human
Cat. No.:
HY-P71101
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