USP10 Protein, Human (sf9, His, GST)

USP10 is a multifunctional hydrolase that plays a critical regulatory role in a variety of cellular processes. It stabilizes the tumor suppressor p53/TP53 by deubiquitinating the tumor suppressor p53/TP53 in the cytoplasm, thereby counteracting MDM2-mediated degradation. USP10 Protein, Human (Sf9, His, GST) is the recombinant human-derived USP10, expressed by Sf9 insect cells, with His, GST labeled tag.

USP10, a hydrolase with versatile substrate specificity, serves as a crucial regulator in multiple cellular processes. Notably, it plays a pivotal role in stabilizing the tumor suppressor p53/TP53 by specifically deubiquitinating it in the cytoplasm under unstressed conditions, counteracting MDM2-mediated degradation. Following DNA damage, USP10 translocates to the nucleus, where it continues to deubiquitinate p53/TP53, contributing to the regulation of the DNA damage response. Additionally, USP10 is integral to a regulatory loop governing autophagy and p53/TP53 levels, mediating the deubiquitination of BECN1. In the context of ribosome biology, USP10 is involved in 40S ribosome subunit recycling, inhibiting stress granule formation and preventing degradation of 40S ribosomal proteins during translation stalling. Beyond its role in protein stability, USP10 participates in endocytic recycling by deubiquitinating CFTR in early endosomes and engages in a TANK-dependent negative feedback response to attenuate NF-kappa-B activation in response to IL1B stimulation or DNA damage. Additionally, USP10 stabilizes TBX21 through deubiquitination, indicating its involvement in diverse cellular pathways.

The fundamental role of USP10 is specific removal of ubiquitin from substrates. USP10 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM.

Human

Sf9 insect cells

His;GST

Q14694 (A2-L798)

9100

Full Length of Mature Protein

ALHSPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAVDKLPDGQEYQRIEFGVDEVIEPSDTLPRTPSYSISSTLNPQAPEFILGCTASKITPDGITKEASYGSIDCQYPGSALALDGSSNVEAEVLENDGVSGGLGQRERKKKKKRPPGYYSYLKDGGDDSISTEALVNGHANSAVPNSVSAEDAEFMGDMPPSVTPRTCNSPQNSTDSVSDIVPDSPFPGALGSDTRTAGQPEGGPGADFGQSCFPAEAGRDTLSRTAGAQPCVGTDTTENLGVANGQILESSGEGTATNGVELHTTESIDLDPTKPESASPPADGTGSASGTLPVSQPKSWASLFHDSKPSSSSPVAYVETKYSPPAISPLVSEKQVEVKEGLVPVSEDPVAIKIAELLENVTLIHKPVSLQPRGLINKGNWCYINATLQALVACPPMYHLMKFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL

Approximately 120 kDa, based on SDS-PAGE under reducing conditions.

≥ 85%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.