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  4. TES

TES is an excellent hydrogen ion buffer. TES has characteristics such as an appropriate dissociation constant, a low metal-binding constant, and high water solubility. TES can be used in the research of mammalian cell culture, viruses, and so on.

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TES

TES Chemische Struktur

CAS. Nr. : 7365-44-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Solution
10 mM * 1 mL in Water Auf Lager
Solid
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Other Forms of TES:

TES Related Antibodies

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Beschreibung

TES is an excellent hydrogen ion buffer. TES has characteristics such as an appropriate dissociation constant, a low metal-binding constant, and high water solubility. TES can be used in the research of mammalian cell culture, viruses, and so on[1][2].

In Vitro

TES (0.1 M) has pKa values of 7.92, 7.50, and 7.14 at 0°, 20° and 37°, respectively, and the concentration of its saturated aqueous solution at 0° is 2.6 M[1].
TES (6-50 mM; 11-14 days) in a closed culture environment is similar to or even more effective than the NaHCO3-CO2-buffered medium in terms of colony-forming efficiency, colony size, and cell morphology of several mammalian cell lines (HeLa S3, KB, BHK 21-Cl3, 3Y1-B Cl-1)[2].
TES (25-50 mM; 14-19 days) has a similar efficiency to NaHCO3-CO2-buffered medium and the required time is shorter in the SV40 virus plaque assay with monkey kidney CV-1 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TES Related Antibodies
Molekulargewicht

229.25

Formel

C6H15NO6S
CAS. Nr.
Appearance

Solid

Color

White to off-white

SMILES

OCC(NCCS(=O)(O)=O)(CO)CO
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Lösungsmittel & Löslichkeit
In Vitro: 

H2O : 125 mg/mL (545.26 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3621 mL 21.8103 mL 43.6205 mL
5 mM 0.8724 mL 4.3621 mL 8.7241 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (436.21 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Reinheit & Dokumentation

Purity: 99.91%

SDS (394 KB)

COA (247 KB) HNMR (236 KB) LCMS (331 KB)

Handling Instructions (2659 KB)
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.3621 mL 21.8103 mL 43.6205 mL 109.0513 mL
5 mM 0.8724 mL 4.3621 mL 8.7241 mL 21.8103 mL
10 mM 0.4362 mL 2.1810 mL 4.3621 mL 10.9051 mL
15 mM 0.2908 mL 1.4540 mL 2.9080 mL 7.2701 mL
20 mM 0.2181 mL 1.0905 mL 2.1810 mL 5.4526 mL
25 mM 0.1745 mL 0.8724 mL 1.7448 mL 4.3621 mL
30 mM 0.1454 mL 0.7270 mL 1.4540 mL 3.6350 mL
40 mM 0.1091 mL 0.5453 mL 1.0905 mL 2.7263 mL
50 mM 0.0872 mL 0.4362 mL 0.8724 mL 2.1810 mL
60 mM 0.0727 mL 0.3635 mL 0.7270 mL 1.8175 mL
80 mM 0.0545 mL 0.2726 mL 0.5453 mL 1.3631 mL
100 mM 0.0436 mL 0.2181 mL 0.4362 mL 1.0905 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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TES Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TES7365-44-8Biochemical Assay ReagentsGood's bufferHeLa S3KBBHK 21-Cl33Y1-B Cl-1CV-1Inhibitorinhibitorinhibit

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