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  2. Biochemical Assay Reagents
  3. Tricine

Tricine is widely used as a buffer in protein electrophoresis, nucleic acid analysis, and immunology, which can help adjust pH and stabilize chemical reactions. In addition, the compound can also be used in research in certain medical fields, such as in antibody detection and drug screening.

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Tricine

Tricine Estructura química

No. CAS : 5704-04-1

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Descripciòn

Tricine is widely used as a buffer in protein electrophoresis, nucleic acid analysis, and immunology, which can help adjust pH and stabilize chemical reactions. In addition, the compound can also be used in research in certain medical fields, such as in antibody detection and drug screening.

In Vitro

Tricine is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

179.17

Fòrmula

C6H13NO5

No. CAS
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CNC(CO)(CO)CO

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad
In Vitro: 

H2O : 25 mg/mL (139.53 mM; Need ultrasonic)

DMSO : 2 mg/mL (11.16 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.5813 mL 27.9065 mL 55.8129 mL
5 mM 1.1163 mL 5.5813 mL 11.1626 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureza y Documentación

Purity: 99.89%

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 5.5813 mL 27.9065 mL 55.8129 mL 139.5323 mL
5 mM 1.1163 mL 5.5813 mL 11.1626 mL 27.9065 mL
10 mM 0.5581 mL 2.7906 mL 5.5813 mL 13.9532 mL
H2O 15 mM 0.3721 mL 1.8604 mL 3.7209 mL 9.3022 mL
20 mM 0.2791 mL 1.3953 mL 2.7906 mL 6.9766 mL
25 mM 0.2233 mL 1.1163 mL 2.2325 mL 5.5813 mL
30 mM 0.1860 mL 0.9302 mL 1.8604 mL 4.6511 mL
40 mM 0.1395 mL 0.6977 mL 1.3953 mL 3.4883 mL
50 mM 0.1116 mL 0.5581 mL 1.1163 mL 2.7906 mL
60 mM 0.0930 mL 0.4651 mL 0.9302 mL 2.3255 mL
80 mM 0.0698 mL 0.3488 mL 0.6977 mL 1.7442 mL
100 mM 0.0558 mL 0.2791 mL 0.5581 mL 1.3953 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

Nombre del producto:
Tricine
Cat. No.:
HY-D0860
Cantidad:
MCE Japan Authorized Agent: