X-alpha-Gal

Cat. No.: HY-114522 Pureza: 99.93%: Ficha de datos
COA

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X-alpha-Gal is a chromogenic substrate that is used to screen colonies with high activity of β-galactopyranoside. X-alpha-Gal also detects α-galactosidase activity. X-alpha-Gal can be used in yeast two hybrid screening for MEL1 gene activation. X-alpha-Gal can be used with Matchmaker GAL4-based products to confirm protein interactions.

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X-alpha-Gal Estructura química

No. CAS : 107021-38-5

Tamaño	Stock	Cantidad

Muestra gratis (0.1 - 0.2 mg)	Solicite ahora
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Descripciòn

Peso molecular

408.63

Fòrmula

C₁₄H₁₅BrClNO₆

No. CAS

107021-38-5

Appearance

Solid

Color

White to light yellow

SMILES

O[C@@H]1[C@@H](O)[C@@H](O)[C@H](O[C@@H]1OC2=CNC3=C2C(Cl)=C(C=C3)Br)CO

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (244.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4472 mL	12.2360 mL	24.4720 mL
5 mM	0.4894 mL	2.4472 mL	4.8944 mL
10 mM	0.2447 mL	1.2236 mL	2.4472 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Pureza y Documentación

Purity: 99.93%

Select Batch:

Ficha de datos (275 KB) SDS (394 KB)

COA (253 KB) HNMR (255 KB) LCMS (94 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. ALAzzE A Y, et al. The effect of chemical mutagenesis on the activity of a-and ß-galactosidases in Lactobacillus reuteri [J]. Milchwissenschaft, 2011, 66: 3.

[2]. Rac, A., & Fulgosi, H. (2019). Qualitative and quantitative dataset of TROL protein interaction with C3 and C4 ferredoxin: NADP+ oxidoreductases. Data in brief, 28, 105038. [Content Brief]

[3]. Aho, S., et al., (1997). A novel reporter gene MEL1 for the yeast two-hybrid system. Analytical biochemistry, 253(2), 270–272. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4472 mL	12.2360 mL	24.4720 mL	61.1800 mL
	5 mM	0.4894 mL	2.4472 mL	4.8944 mL	12.2360 mL
	10 mM	0.2447 mL	1.2236 mL	2.4472 mL	6.1180 mL
	15 mM	0.1631 mL	0.8157 mL	1.6315 mL	4.0787 mL
	20 mM	0.1224 mL	0.6118 mL	1.2236 mL	3.0590 mL
	25 mM	0.0979 mL	0.4894 mL	0.9789 mL	2.4472 mL
	30 mM	0.0816 mL	0.4079 mL	0.8157 mL	2.0393 mL
	40 mM	0.0612 mL	0.3059 mL	0.6118 mL	1.5295 mL
	50 mM	0.0489 mL	0.2447 mL	0.4894 mL	1.2236 mL
	60 mM	0.0408 mL	0.2039 mL	0.4079 mL	1.0197 mL
	80 mM	0.0306 mL	0.1530 mL	0.3059 mL	0.7648 mL
	100 mM	0.0245 mL	0.1224 mL	0.2447 mL	0.6118 mL

X-alpha-Gal Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

X-alpha-Gal107021-38-5Biochemical Assay ReagentschromogenicsubstrateInhibitorinhibitorinhibit

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X-alpha-Gal

X-alpha-Gal Related Antibodies

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