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  4. 3,3'-Dihexyloxacarbocyanine iodide

3,3'-Dihexyloxacarbocyanine iodide  (Synonyms: DiOC6(3) iodide)

Cat. No.: HY-D0084 Purity: 99.17%
COA
SDS
Handling Instructions Technical Support

3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.

For research use only. We do not sell to patients.

3,3'-Dihexyloxacarbocyanine iodide

3,3'-Dihexyloxacarbocyanine iodide Chemical Structure

CAS No. : 53213-82-4

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

3,3'-Dihexyloxacarbocyanine iodide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 3,3'-Dihexyloxacarbocyanine iodide

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.

In Vitro

Addition of cells to the cuvette containing 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) leads to an increase in fluorescence, equilibration is rapid and is complete by 4 min. When f-met-leu-phe (10-7 M) is added to neutrophils preequilibrated with 3,3'-Dihexyloxacarbocyanine iodide, there is a short lag period of 5 seconds. The lag period is followed by a rapid loss of fluorescence. Examination of 3,3'-Dihexyloxacarbocyanine iodide loaded neutrophils using fluorescence microscopy demonstrates that in resting neutrophils the brightest fluorescence is associated with long thin organelles which corresponds to the distribution of mitochondria[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3,3'-Dihexyloxacarbocyanine iodide Related Antibodies
Molecular Weight

572.52

Formula

C29H37IN2O2
CAS No.
Appearance

Solid

Color

Orange to red

Emission (Em)

515
Excitation (Ex)

486
SMILES

CCCCCCN1/C(OC2=CC=CC=C12)=C/C=C/C3=[N+](CCCCCC)C4=CC=CC=C4O3.[I-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (58.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7467 mL 8.7333 mL 17.4666 mL
5 mM 0.3493 mL 1.7467 mL 3.4933 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.62%

SDS (393 KB)

COA (258 KB) HNMR (279 KB) LCMS (100 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

Neutrophils (2.5×106/mL) are preequilibrated in 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) for 5 min at 37°C in a 1 cm path length cuvette. Final volume is 2 mL. Fluorescence measurements are made in a spectrofluorimeter (emission wavelength: 510 nm, excitation wavelength: 470 nm). Stimuli are injected by a syringe through an injection port on the spectrofluorimeter, and their volumes never exceed 5% (v/v)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7467 mL 8.7333 mL 17.4666 mL 43.6666 mL
5 mM 0.3493 mL 1.7467 mL 3.4933 mL 8.7333 mL
10 mM 0.1747 mL 0.8733 mL 1.7467 mL 4.3667 mL
15 mM 0.1164 mL 0.5822 mL 1.1644 mL 2.9111 mL
20 mM 0.0873 mL 0.4367 mL 0.8733 mL 2.1833 mL
25 mM 0.0699 mL 0.3493 mL 0.6987 mL 1.7467 mL
30 mM 0.0582 mL 0.2911 mL 0.5822 mL 1.4556 mL
40 mM 0.0437 mL 0.2183 mL 0.4367 mL 1.0917 mL
50 mM 0.0349 mL 0.1747 mL 0.3493 mL 0.8733 mL
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3,3'-Dihexyloxacarbocyanine iodide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3,3'-Dihexyloxacarbocyanine53213-82-4DiOC6(3)Fluorescent DyeInhibitorinhibitorinhibit

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