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  4. 3,3'-Dihexyloxacarbocyanine iodide

3,3'-Dihexyloxacarbocyanine iodide  (Synonyms: DiOC6(3) iodide; 3,3′-ジヘキシルオキサカルボシアニンヨージド)

製品番号: HY-D0084 純度: 99.17%
COA 取扱説明書 Technical Support

3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.

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3,3'-Dihexyloxacarbocyanine iodide

3,3'-Dihexyloxacarbocyanine iodide 構造式

CAS 番号 : 53213-82-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 在庫あり
Solution
10 mM * 1 mL in DMSO USD 66 在庫あり
Solid
50 mg $60 在庫あり
100 mg   お問い合わせ  
200 mg   お問い合わせ  

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Based on 1 publication(s) in Google Scholar

3,3'-Dihexyloxacarbocyanine iodide 関連抗体

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製品説明

3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.

体外実験

Addition of cells to the cuvette containing 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) leads to an increase in fluorescence, equilibration is rapid and is complete by 4 min. When f-met-leu-phe (10-7 M) is added to neutrophils preequilibrated with 3,3'-Dihexyloxacarbocyanine iodide, there is a short lag period of 5 seconds. The lag period is followed by a rapid loss of fluorescence. Examination of 3,3'-Dihexyloxacarbocyanine iodide loaded neutrophils using fluorescence microscopy demonstrates that in resting neutrophils the brightest fluorescence is associated with long thin organelles which corresponds to the distribution of mitochondria[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3,3'-Dihexyloxacarbocyanine iodide 関連抗体
分子量

572.52

分子式

C29H37IN2O2
Emission (Em)

515
Excitation (Ex)

486
CAS 番号
Appearance

Solid

Color

Orange to red

SMILES

CCCCCCN1/C(OC2=CC=CC=C12)=C/C=C/C3=[N+](CCCCCC)C4=CC=CC=C4O3.[I-]
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
体外: 

DMSO : 33.33 mg/mL (58.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7467 mL 8.7333 mL 17.4666 mL
5 mM 0.3493 mL 1.7467 mL 3.4933 mL
10 mM 0.1747 mL 0.8733 mL 1.7467 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

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体積 （開始）

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体積 （終了）

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
純度とドキュメンテーション

純度: 99.62%

COA (258 KB) HNMR (279 KB) LCMS (100 KB)

取扱説明書 (2659 KB)
参考文献
細胞実験
[1]

Neutrophils (2.5×106/mL) are preequilibrated in 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) for 5 min at 37°C in a 1 cm path length cuvette. Final volume is 2 mL. Fluorescence measurements are made in a spectrofluorimeter (emission wavelength: 510 nm, excitation wavelength: 470 nm). Stimuli are injected by a syringe through an injection port on the spectrofluorimeter, and their volumes never exceed 5% (v/v)[1].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7467 mL 8.7333 mL 17.4666 mL 43.6666 mL
5 mM 0.3493 mL 1.7467 mL 3.4933 mL 8.7333 mL
10 mM 0.1747 mL 0.8733 mL 1.7467 mL 4.3667 mL
15 mM 0.1164 mL 0.5822 mL 1.1644 mL 2.9111 mL
20 mM 0.0873 mL 0.4367 mL 0.8733 mL 2.1833 mL
25 mM 0.0699 mL 0.3493 mL 0.6987 mL 1.7467 mL
30 mM 0.0582 mL 0.2911 mL 0.5822 mL 1.4556 mL
40 mM 0.0437 mL 0.2183 mL 0.4367 mL 1.0917 mL
50 mM 0.0349 mL 0.1747 mL 0.3493 mL 0.8733 mL
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3,3'-Dihexyloxacarbocyanine iodide Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3,3'-Dihexyloxacarbocyanine53213-82-4DiOC6(3)Fluorescent DyeInhibitorinhibitorinhibit

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製品名:
3,3'-Dihexyloxacarbocyanine iodide
製品番号:
HY-D0084
数量:
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