3,5-Dinitrosalicylic acid

Name: 3,5-Dinitrosalicylic acid
Brand: MedChemExpress
SKU: HY-W009694
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Cat. No.: HY-W009694 Pureza: 99.97%: Ficha de datos
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3,5-Dinitrosalicylic acid the derivative of salicylic acid. 3,5-Dinitrosalicylic acid is used in the α-amylase assay, carbohydrase assay, and for the colorimetric determination of reducing substances.

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3,5-Dinitrosalicylic acid Estructura química

No. CAS : 609-99-4

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
25 g	En stock	Estimated Time of Arrival: December 31
100 g	En stock	Estimated Time of Arrival: December 31
> 100 g	Obtener un presupuesto

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Descripciòn

In Vitro

3,5-Dinitrosalicylic acid is a commonly used reducing sugar detection reagent. 3,5-Dinitrosalicylic acid undergoes an oxidation-reduction reaction with reducing sugars to generate derivatives, thereby producing a color change for quantitative detection of reducing sugar content.
Two different DNSA reagents were prepared:
1. Reagent preparation:
DNSA-1 reagent: 0.4M NaOH solution, and each 100mL solution contains a certain amount of heptahydrate.
DNSA-2 reagent: 0.7M NaOH solution, Each 100mL contains 2g 3,5-dinitrosalicylic acid.
2.Sample preparation:
Prepare a series of maltodextrin solutions of different concentrations (0.15 - 5.85mM). If the effect of calcium ions is to be studied, calcium chloride is added to the maltodextrin solution to a concentration of (40mM).
3.Test steps:
Take 2mL of sample and place it in a Folin-Wu sugar tube and add DNSA reagent. Heat the solution in a boiling water bath for precisely 5min and then cool it in cold running water for 5min. After cooling, dilute the solution to 25mL and measure the absorbance using a Unicam SP 1300 colorimeter with a green filter.
4.Result analysis:
Plot a curve with absorbance (A) as the ordinate and millimolar concentration of maltodextrin (M) as the abscissa. Compare the molar reduction values of different maltodextrins by plotting the slope of the absorbance-concentration curve of each maltodextrin versus the size (number of glucose residues) of the maltodextrin. If the absorbance value of the reagent is proportional to the number of reducing ends, the slope of the curve is zero; if the absorbance value is not proportional to the number of reducing ends and increases with the size of maltodextrin, the slope of the curve is greater than zero.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

228.12

Fòrmula

C₇H₄N₂O₇

No. CAS

609-99-4

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C(O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvente y solubilidad

In Vitro:

DMSO : 175 mg/mL (767.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3837 mL	21.9187 mL	43.8373 mL
5 mM	0.8767 mL	4.3837 mL	8.7675 mL
10 mM	0.4384 mL	2.1919 mL	4.3837 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

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Calculation results:

Working solution concentration: mg/mL

Pureza y Documentación

Purity: 99.97%

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Ficha de datos (276 KB) SDS (604 KB)

COA (253 KB) HNMR (246 KB) LCMS (91 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Robyt JF, et al., Reducing value methods for maltodextrins. I. Chain-length dependence of alkaline 3,5-dinitrosalicylate and chain-length independence of alkaline copper. Anal Biochem. 1972 Feb;45(2):510-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.3837 mL	21.9187 mL	43.8373 mL	109.5934 mL
	5 mM	0.8767 mL	4.3837 mL	8.7675 mL	21.9187 mL
	10 mM	0.4384 mL	2.1919 mL	4.3837 mL	10.9593 mL
	15 mM	0.2922 mL	1.4612 mL	2.9225 mL	7.3062 mL
	20 mM	0.2192 mL	1.0959 mL	2.1919 mL	5.4797 mL
	25 mM	0.1753 mL	0.8767 mL	1.7535 mL	4.3837 mL
	30 mM	0.1461 mL	0.7306 mL	1.4612 mL	3.6531 mL
	40 mM	0.1096 mL	0.5480 mL	1.0959 mL	2.7398 mL
	50 mM	0.0877 mL	0.4384 mL	0.8767 mL	2.1919 mL
	60 mM	0.0731 mL	0.3653 mL	0.7306 mL	1.8266 mL
	80 mM	0.0548 mL	0.2740 mL	0.5480 mL	1.3699 mL
	100 mM	0.0438 mL	0.2192 mL	0.4384 mL	1.0959 mL