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  4. 4-Azido-L-phenylalanine

4-Azido-L-phenylalanine  (Synonyms: p-Azidophenylalanine; p-Azido-L-phenylalanine)

Cat. No.: HY-16714 Purity: 99.93%
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Handling Instructions Technical Support

4-Azido-L-phenylalanine is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments. 4-Azido-L-phenylalanine is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

For research use only. We do not sell to patients.

CAS No. : 33173-53-4

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of 4-Azido-L-phenylalanine:

4-Azido-L-phenylalanine Related Antibodies

Top Publications Citing Use of Products

    4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197.  [Abstract]

    4-Azido-L-phenylalanine (Azi) (1 mM). The expression of mutated VSV-G proteins (mProteins) in HEK 293T cells was detected by Western blot.

    4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197.  [Abstract]

    The site-specific display of 4-Azido-L-phenylalanine (Azi) (1 mM) at site 192 in mProtein D192, as a typical site, was verified by LC-MS/MS spectrum of a peptide derived from the purified protein after trypsinization.

    4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197.  [Abstract]

    4-Azido-L-phenylalanine (Azi) (1 mM). The membrane proteins of mBiosomes were extracted and analyzed by Western blot.

    4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197.  [Abstract]

    The introduction of 4-Azido-L-phenylalanine (Azi) (1 mM) to site 246 caused evident changes in the secondary structures of the surrounding residues in mProtein A246.

    4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197.  [Abstract]

    4-Azido-L-phenylalanine (Azi) (1 mM). The proportion % of the secondary structures, including β-turn, helix, and random coil at surrounding sites (sites 245, 246, 247 and 248) in unmutated VSV-G, mProtein A246 and mProtein A247.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    4-Azido-L-phenylalanine is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments. 4-Azido-L-phenylalanine is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

    In Vitro

    In cell-free protein synthesis (CFPS) method, 0.9-1.7 mg/mL of modified soluble super-folder green fluorescent protein (sfGFP) containing either 4-Azido-L-phenylalanine or p-propargyloxy-l-phenylalanine (pPaF) accumulate in the CFPS solutions[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    4-Azido-L-phenylalanine Related Antibodies
    Molecular Weight

    206.20

    Formula

    C9H10N4O2
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=C(O)[C@@H](N)CC1=CC=C(N=[N+]=[N-])C=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (242.48 mM; ultrasonic and adjust pH to 13 with NaOH)

    H2O : 25 mg/mL (121.24 mM; ultrasonic and adjust pH to 11 with NaOH)

    DMSO : 5 mg/mL (24.25 mM; ultrasonic and warming and adjust pH to 3 with HCl and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.8497 mL 24.2483 mL 48.4966 mL
    5 mM 0.9699 mL 4.8497 mL 9.6993 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.93%

    SDS (406 KB)

    COA (250 KB) HNMR (403 KB) RP-HPLC (275 KB) NP-HPLC (242 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O / H2O 1 mM 4.8497 mL 24.2483 mL 48.4966 mL 121.2415 mL
    5 mM 0.9699 mL 4.8497 mL 9.6993 mL 24.2483 mL
    10 mM 0.4850 mL 2.4248 mL 4.8497 mL 12.1242 mL
    15 mM 0.3233 mL 1.6166 mL 3.2331 mL 8.0828 mL
    20 mM 0.2425 mL 1.2124 mL 2.4248 mL 6.0621 mL
    H2O / H2O 25 mM 0.1940 mL 0.9699 mL 1.9399 mL 4.8497 mL
    30 mM 0.1617 mL 0.8083 mL 1.6166 mL 4.0414 mL
    40 mM 0.1212 mL 0.6062 mL 1.2124 mL 3.0310 mL
    50 mM 0.0970 mL 0.4850 mL 0.9699 mL 2.4248 mL
    60 mM 0.0808 mL 0.4041 mL 0.8083 mL 2.0207 mL
    80 mM 0.0606 mL 0.3031 mL 0.6062 mL 1.5155 mL
    100 mM 0.0485 mL 0.2425 mL 0.4850 mL 1.2124 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    4-Azido-L-phenylalanine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    4-Azido-L-phenylalanine33173-53-4p-Azidophenylalanine p-Azido-L-phenylalanineBiochemical Assay ReagentsInhibitorinhibitorinhibit

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