4-Azido-L-phenylalanine hydrochloride
Based on 9 publication(s) in Google Scholar
4-Azido-L-phenylalanine hydrochloride is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments. 4-Azido-L-phenylalanine (hydrochloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.88%
- CAS 番号: 34670-43-4
- 分子式: C9H11ClN4O2
- 分子量:242.66
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保管条件:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 4-Azido-L-phenylalanine hydrochloride
More- Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
- Sci Adv. 2025 Jun 13;11(24):eadt1346. [Abstract]
- Cell Chem Biol. 2021 Sep 16;28(9):1366-1378.e4. [Abstract]
- Emerg Microbes Infect. 2021 Dec;10(1):1609-1625. [Abstract]
- Bioconjug Chem. 2019 Dec 18;30(12):2998-3006. [Abstract]
- RSC Chem Biol. 2025 Apr 21;6(6):860-868. [Abstract]
- Protein Expr Purif. 2022 Dec:200:106158. [Abstract]
- bioRxiv. 2024 Feb 12.
- Elife. 2021 Jun 1;10:e67789. [Abstract]
Customer Validation & Images
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WB
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Bio/Physico-chemical Assay
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WB
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
生物活性
In cell-free protein synthesis (CFPS) method, 0.9-1.7 mg/mL of modified soluble super-folder green fluorescent protein (sfGFP) containing either 4-Azido-L-phenylalanine or p-propargyloxy-l-phenylalanine (pPaF) accumulate in the CFPS solutions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 34670-43-4
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性状 Solid
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分子量 242.66
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分子式 C9H11ClN4O2
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Color Off-white to light yellow
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SMILES
O=C(O)[C@@H](N)CC1=CC=C(N=[N+]=[N-])C=C1.Cl
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別名
p-Azidophenylalanine hydrochloride; p-Azido-L-phenylalanine hydrochloride
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (9)
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Journal Impact Factor
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Most Recent
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Nanomicro Lett
2023 Aug 12;15(1):197. PMID: 37572220
4-Azido-L-phenylalanine hydrochloride purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
4-Azido-L-phenylalanine (Azi) (1 mM). The expression of mutated VSV-G proteins (mProteins) in HEK 293T cells was detected by Western blot.
4-Azido-L-phenylalanine hydrochloride purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
The site-specific display of 4-Azido-L-phenylalanine (Azi) (1 mM) at site 192 in mProtein D192, as a typical site, was verified by LC-MS/MS spectrum of a peptide derived from the purified protein after trypsinization.
4-Azido-L-phenylalanine hydrochloride purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
4-Azido-L-phenylalanine (Azi) (1 mM). The membrane proteins of mBiosomes were extracted and analyzed by Western blot.
4-Azido-L-phenylalanine hydrochloride purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
The introduction of 4-Azido-L-phenylalanine (Azi) (1 mM) to site 246 caused evident changes in the secondary structures of the surrounding residues in mProtein A246.
4-Azido-L-phenylalanine hydrochloride purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
4-Azido-L-phenylalanine (Azi) (1 mM). The proportion % of the secondary structures, including β-turn, helix, and random coil at surrounding sites (sites 245, 246, 247 and 248) in unmutated VSV-G, mProtein A246 and mProtein A247.
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Sci Adv
2025 Jun 13;11(24):eadt1346. PMID: 40498833 -
Cell Chem Biol
2021 Sep 16;28(9):1366-1378.e4. PMID: 33798447 -
Emerg Microbes Infect
2021 Dec;10(1):1609-1625. PMID: 34340648 -
Bioconjug Chem
Interactions between PHD3-Bromo of MLL1 and H3K4me3 Revealed by Single-Molecule Magnetic Tweezers in a Parallel DNA Circuit. [Abstract]2019 Dec 18;30(12):2998-3006. PMID: 31714753 -
RSC Chem Biol
Monitoring SARS-CoV-2 Nsp13 helicase binding activity using expanded genetic code techniques. [Abstract]2025 Apr 21;6(6):860-868. PMID: 40309067 -
Protein Expr Purif
Efficient incorporation of p-azido-l-phenylalanine into the protein using organic solvents. [Abstract]2022 Dec:200:106158. PMID: 36007861 -
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Elife
Genetic code expansion enables visualization of Salmonella type three secretion system components and secreted effectors. [Abstract]2021 Jun 1;10:e67789. PMID: 34061032
溶剤 & 溶解度
DMSO : 100 mg/mL (412.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 10 mg/mL (41.21 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.30 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.30 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 16.67 mg/mL (68.70 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.1210 mL | 20.6050 mL | 41.2099 mL | 103.0248 mL |
| 5 mM | 0.8242 mL | 4.1210 mL | 8.2420 mL | 20.6050 mL | |
| 10 mM | 0.4121 mL | 2.0605 mL | 4.1210 mL | 10.3025 mL | |
| 15 mM | 0.2747 mL | 1.3737 mL | 2.7473 mL | 6.8683 mL | |
| 20 mM | 0.2060 mL | 1.0302 mL | 2.0605 mL | 5.1512 mL | |
| 25 mM | 0.1648 mL | 0.8242 mL | 1.6484 mL | 4.1210 mL | |
| 30 mM | 0.1374 mL | 0.6868 mL | 1.3737 mL | 3.4342 mL | |
| 40 mM | 0.1030 mL | 0.5151 mL | 1.0302 mL | 2.5756 mL | |
| DMSO | 50 mM | 0.0824 mL | 0.4121 mL | 0.8242 mL | 2.0605 mL |
| 60 mM | 0.0687 mL | 0.3434 mL | 0.6868 mL | 1.7171 mL | |
| 80 mM | 0.0515 mL | 0.2576 mL | 0.5151 mL | 1.2878 mL | |
| 100 mM | 0.0412 mL | 0.2060 mL | 0.4121 mL | 1.0302 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
4-Azido-L-phenylalanine hydrochloride 関連分類
HY-16714A 関連分類
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