4-Azido-L-phenylalanine
Based on 9 publication(s) in Google Scholar
4-Azido-L-phenylalanine is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments. 4-Azido-L-phenylalanine is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 33173-53-4
- Formula: C9H10N4O2
- Molecular Weight:206.20
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 4-Azido-L-phenylalanine
More- Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
- Sci Adv. 2025 Jun 13;11(24):eadt1346. [Abstract]
- Emerg Microbes Infect. 2021 Dec;10(1):1609-1625. [Abstract]
- Cell Chem Biol. 2021 Sep 16;28(9):1366-1378.e4. [Abstract]
- Elife. 2021 Jun 1;10:e67789. [Abstract]
- Bioconjug Chem. 2019 Dec 18;30(12):2998-3006. [Abstract]
- RSC Chem Biol. 2025 Apr 21;6(6):860-868. [Abstract]
- Protein Expr Purif. 2022 Dec:200:106158. [Abstract]
- bioRxiv. 2024 Feb 12.
Customer Validation & Images
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WB
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Bio/Physico-chemical Assay
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WB
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
Biological Activity
In cell-free protein synthesis (CFPS) method, 0.9-1.7 mg/mL of modified soluble super-folder green fluorescent protein (sfGFP) containing either 4-Azido-L-phenylalanine or p-propargyloxy-l-phenylalanine (pPaF) accumulate in the CFPS solutions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 33173-53-4
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Appearance Solid
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Molecular Weight 206.20
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Formula C9H10N4O2
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Color White to yellow
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SMILES
O=C(O)[C@@H](N)CC1=CC=C(N=[N+]=[N-])C=C1
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Synonyms
p-Azidophenylalanine; p-Azido-L-phenylalanine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (9)
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Journal Impact Factor
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Most Recent
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Nanomicro Lett
2023 Aug 12;15(1):197. PMID: 37572220
4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
4-Azido-L-phenylalanine (Azi) (1 mM). The expression of mutated VSV-G proteins (mProteins) in HEK 293T cells was detected by Western blot.
4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
The site-specific display of 4-Azido-L-phenylalanine (Azi) (1 mM) at site 192 in mProtein D192, as a typical site, was verified by LC-MS/MS spectrum of a peptide derived from the purified protein after trypsinization.
4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
4-Azido-L-phenylalanine (Azi) (1 mM). The membrane proteins of mBiosomes were extracted and analyzed by Western blot.
4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
The introduction of 4-Azido-L-phenylalanine (Azi) (1 mM) to site 246 caused evident changes in the secondary structures of the surrounding residues in mProtein A246.
4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]
4-Azido-L-phenylalanine (Azi) (1 mM). The proportion % of the secondary structures, including β-turn, helix, and random coil at surrounding sites (sites 245, 246, 247 and 248) in unmutated VSV-G, mProtein A246 and mProtein A247.
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Sci Adv
2025 Jun 13;11(24):eadt1346. PMID: 40498833 -
Emerg Microbes Infect
2021 Dec;10(1):1609-1625. PMID: 34340648 -
Cell Chem Biol
2021 Sep 16;28(9):1366-1378.e4. PMID: 33798447 -
Elife
Genetic code expansion enables visualization of Salmonella type three secretion system components and secreted effectors. [Abstract]2021 Jun 1;10:e67789. PMID: 34061032 -
Bioconjug Chem
Interactions between PHD3-Bromo of MLL1 and H3K4me3 Revealed by Single-Molecule Magnetic Tweezers in a Parallel DNA Circuit. [Abstract]2019 Dec 18;30(12):2998-3006. PMID: 31714753 -
RSC Chem Biol
Monitoring SARS-CoV-2 Nsp13 helicase binding activity using expanded genetic code techniques. [Abstract]2025 Apr 21;6(6):860-868. PMID: 40309067 -
Protein Expr Purif
Efficient incorporation of p-azido-l-phenylalanine into the protein using organic solvents. [Abstract]2022 Dec:200:106158. PMID: 36007861 -
Solvent & Solubility
H2O : 50 mg/mL (242.48 mM; ultrasonic and adjust pH to 13 with NaOH)
H2O : 25 mg/mL (121.24 mM; ultrasonic and adjust pH to 11 with NaOH)
DMSO : 5 mg/mL (24.25 mM; ultrasonic and warming and adjust pH to 3 with HCl and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (406 KB)
- English - EN (406 KB)
- Français - FR (406 KB)
- Deutsch - DE (406 KB)
- Norwegian - NO (406 KB)
- Español - ES (406 KB)
- Swedish - SV (406 KB)
- Italian - IT (406 KB)
- Portuguese - PT (406 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O / H2O | 1 mM | 4.8497 mL | 24.2483 mL | 48.4966 mL | 121.2415 mL |
| 5 mM | 0.9699 mL | 4.8497 mL | 9.6993 mL | 24.2483 mL | |
| 10 mM | 0.4850 mL | 2.4248 mL | 4.8497 mL | 12.1242 mL | |
| 15 mM | 0.3233 mL | 1.6166 mL | 3.2331 mL | 8.0828 mL | |
| 20 mM | 0.2425 mL | 1.2124 mL | 2.4248 mL | 6.0621 mL | |
| H2O / H2O | 25 mM | 0.1940 mL | 0.9699 mL | 1.9399 mL | 4.8497 mL |
| 30 mM | 0.1617 mL | 0.8083 mL | 1.6166 mL | 4.0414 mL | |
| 40 mM | 0.1212 mL | 0.6062 mL | 1.2124 mL | 3.0310 mL | |
| 50 mM | 0.0970 mL | 0.4850 mL | 0.9699 mL | 2.4248 mL | |
| 60 mM | 0.0808 mL | 0.4041 mL | 0.8083 mL | 2.0207 mL | |
| 80 mM | 0.0606 mL | 0.3031 mL | 0.6062 mL | 1.5155 mL | |
| 100 mM | 0.0485 mL | 0.2425 mL | 0.4850 mL | 1.2124 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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