Art. -Nr.: HY-16714

4-Azido-L-phenylalanine

Name: 4-Azido-L-phenylalanine
Brand: MedChemExpress
SKU: HY-16714
Price: 98.00 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: p-Azidophenylalanine; p-Azido-L-phenylalanine)

10 Cited Publications

Kundenbewertung

Based on 10 publication(s) in Google Scholar

4-Azido-L-phenylalanine is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments. 4-Azido-L-phenylalanine is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

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Reinheit : 99.93%
CAS. Nr.: 33173-53-4
Formel: C₉H₁₀N₄O₂
Molecular Weight:206.20

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

4-Azido-L-phenylalanine Chemical Structure

CAS. Nr.: 33173-53-4

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (10)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

In cell-free protein synthesis (CFPS) method, 0.9-1.7 mg/mL of modified soluble super-folder green fluorescent protein (sfGFP) containing either 4-Azido-L-phenylalanine or p-propargyloxy-l-phenylalanine (pPaF) accumulate in the CFPS solutions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)

Chemical Information

CAS. Nr. 33173-53-4
Appearance Solid
Molecular Weight 206.20
Formel C₉H₁₀N₄O₂
Color White to yellow
SMILES

O=C(O)[C@@H](N)CC1=CC=C(N=[N+]=[N-])C=C1
Synonyms

p-Azidophenylalanine; p-Azido-L-phenylalanine
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (10)

All WBBio/Physico-chemical Assay

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Journal Impact Factor

Nanomicro Lett

Bioorthogonal Engineered Virus-Like Nanoparticles for Efficient Gene Therapy. [Abstract]

2023 Aug 12;15(1):197. PMID: 37572220

4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]

4-Azido-L-phenylalanine (Azi) (1 mM). The expression of mutated VSV-G proteins (mProteins) in HEK 293T cells was detected by Western blot.

4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]

The site-specific display of 4-Azido-L-phenylalanine (Azi) (1 mM) at site 192 in mProtein D192, as a typical site, was verified by LC-MS/MS spectrum of a peptide derived from the purified protein after trypsinization.

4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]

4-Azido-L-phenylalanine (Azi) (1 mM). The membrane proteins of mBiosomes were extracted and analyzed by Western blot.

4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]

The introduction of 4-Azido-L-phenylalanine (Azi) (1 mM) to site 246 caused evident changes in the secondary structures of the surrounding residues in mProtein A246.

4-Azido-L-phenylalanine purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]

4-Azido-L-phenylalanine (Azi) (1 mM). The proportion % of the secondary structures, including β-turn, helix, and random coil at surrounding sites (sites 245, 246, 247 and 248) in unmutated VSV-G, mProtein A246 and mProtein A247.
Sci Adv

ATP13A1 engages SEC61 to facilitate substrate-specific translocation. [Abstract]

2025 Jun 13;11(24):eadt1346. PMID: 40498833
Cell Chem Biol

Bioorthogonal dissection of the replicase assembly of hepatitis C virus. [Abstract]

2021 Sep 16;28(9):1366-1378.e4. PMID: 33798447
Emerg Microbes Infect

NS5-independent Ablation of STAT2 by Zika virus to antagonize interferon signalling. [Abstract]

2021 Dec;10(1):1609-1625. PMID: 34340648
Protein Sci

2026 Aug;35(8):e70684. PMID: 42438363
Bioconjug Chem

Interactions between PHD3-Bromo of MLL1 and H3K4me3 Revealed by Single-Molecule Magnetic Tweezers in a Parallel DNA Circuit. [Abstract]

2019 Dec 18;30(12):2998-3006. PMID: 31714753
RSC Chem Biol

Monitoring SARS-CoV-2 Nsp13 helicase binding activity using expanded genetic code techniques. [Abstract]

2025 Apr 21;6(6):860-868. PMID: 40309067
Protein Expr Purif

Efficient incorporation of p-azido-l-phenylalanine into the protein using organic solvents. [Abstract]

2022 Dec:200:106158. PMID: 36007861
bioRxiv

2024 Feb 12.
Elife

Genetic code expansion enables visualization of Salmonella type three secretion system components and secreted effectors. [Abstract]

2021 Jun 1;10:e67789. PMID: 34061032

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 50 mg/mL (242.48 mM; ultrasonic and adjust pH to 13 with NaOH)

H₂O : 25 mg/mL (121.24 mM; ultrasonic and adjust pH to 11 with NaOH)

DMSO : 5 mg/mL (24.25 mM; ultrasonic and warming and adjust pH to 3 with HCl and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.8497 mL

24.2483 mL

48.4966 mL

5 mM

0.9699 mL

4.8497 mL

9.6993 mL

10 mM

0.4850 mL

2.4248 mL

4.8497 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (406 KB)
- English - EN (406 KB)
- Français - FR (406 KB)
- Deutsch - DE (406 KB)
- Norwegian - NO (406 KB)
- Español - ES (406 KB)
- Swedish - SV (406 KB)
- Italian - IT (406 KB)
- Korean - KR (406 KB)
- Portuguese - PT (406 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Albayrak C, et al. Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation. Nucleic Acids Res. 2013 Jun;41(11):5949-63. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O / H₂O	1 mM	4.8497 mL	24.2483 mL	48.4966 mL	121.2415 mL
	5 mM	0.9699 mL	4.8497 mL	9.6993 mL	24.2483 mL
	10 mM	0.4850 mL	2.4248 mL	4.8497 mL	12.1242 mL
	15 mM	0.3233 mL	1.6166 mL	3.2331 mL	8.0828 mL
	20 mM	0.2425 mL	1.2124 mL	2.4248 mL	6.0621 mL
H₂O / H₂O	25 mM	0.1940 mL	0.9699 mL	1.9399 mL	4.8497 mL
	30 mM	0.1617 mL	0.8083 mL	1.6166 mL	4.0414 mL
	40 mM	0.1212 mL	0.6062 mL	1.2124 mL	3.0310 mL
	50 mM	0.0970 mL	0.4850 mL	0.9699 mL	2.4248 mL
	60 mM	0.0808 mL	0.4041 mL	0.8083 mL	2.0207 mL
	80 mM	0.0606 mL	0.3031 mL	0.6062 mL	1.5155 mL
	100 mM	0.0485 mL	0.2425 mL	0.4850 mL	1.2124 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bioz

Keywords

4-Azido-L-phenylalanine

10 Cited Publications

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4-Azido-L-phenylalanine

Publications Citing Use of MedChemExpress (MCE) 4-Azido-L-phenylalanine