2. NF-κB
  3. Keap1-Nrf2
  4. 4-Octyl Itaconate

4-Octyl Itaconate 

Cat. No.: HY-112675 Purity: 99.98%
COA Handling Instructions

4-Octyl Itaconate is a cell-permeable Itaconate derivative. Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1.

For research use only. We do not sell to patients.

4-Octyl Itaconate Chemical Structure

4-Octyl Itaconate Chemical Structure

CAS No. : 3133-16-2

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Free Sample (0.1 - 0.5 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 55 In-stock
Estimated Time of Arrival: December 31
Solid
50 mg USD 50 In-stock
Estimated Time of Arrival: December 31
100 mg USD 90 In-stock
Estimated Time of Arrival: December 31
200 mg USD 160 In-stock
Estimated Time of Arrival: December 31
500 mg USD 360 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 47 publication(s) in Google Scholar

Top Publications Citing Use of Products

32 Publications Citing Use of MCE 4-Octyl Itaconate

Proliferation Assay
RT-PCR
WB
IF

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Cell Rep. 2023 Feb 28;42(3):112145.  [Abstract]

    4-Octyl Itaconate (4-OI; 250 µM; 2 h) significantly inhibits the phosphorylation of STING and the activation of downstream signaling molecules in RAW264.7 macrophages.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Int J Oral Sci. 2022 May 31;14(1):27.  [Abstract]

    4-octyl itaconate (4-OI; 200 μM) induces a shift in polarization phenotype of macrophages in vitro. Expression of M1 and M2-related genes in RAW 264.7 using qRT-PCR (The M1-related IL-1β, IL-6, CCL2, iNOS, CD86, and the M2-related TGF-β, Arg-1 and CD206).

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Int J Oral Sci. 2022 May 31;14(1):27.  [Abstract]

    The role of Nrf2 exerted in the protection of 4-octyl itaconate (4-OI; 200 μM) against periodontal inflammation. a The Nrf2 protein expression in nuclei fraction lysates quantified by Western blotting. The expression of Nrf2 downstream ARE-dependent proteins in RAW264.7 measured by Western blotting.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Int J Oral Sci. 2022 May 31;14(1):27.  [Abstract]

    Hematoxylin and eosin (H&E) staining indicated 4-octyl itaconate (4-OI) (50 mg/kg; injected locally; daily for 14 days ) could play a pivotal role in decreasing alveolar bone loss in experimental periodontitis models.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Int J Oral Sci. 2022 May 31;14(1):27.  [Abstract]

    4-octyl itaconate (4-OI; 200 μM) induces a shift in polarization phenotype of macrophages in vitro. Micrographs showing staining of iNOS, CD86 and CD206 obtained using confocal laser scanning microscopy.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Cell Death Dis. 2022 Feb 10;13(2):140.  [Abstract]

    Evaluated the effect of 4-Octyl Itaconate (4-OI; 20-80 μM) on the LPS-induced inflammation model. The protein expression of iNOS, COX2 and IL-6 are measured by western blot.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Cell Death Dis. 2022 Feb 10;13(2):140.  [Abstract]

    Cell Counting Kit-8 (CCK-8) results for microglia treated with different concentrations of 4-Octyl Itaconate (4-OI) for 24 and 48 h.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Cell Death Dis. 2022 Feb 10;13(2):140.  [Abstract]

    Evaluated the effect of 4-Octyl Itaconate (4-OI; 20-80 μM) on the LPS-induced inflammation model. The mRNA expression of iNOS, COX2 and IL-6 are measured by qRT-PCR.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Cell Death Dis. 2022 Feb 10;13(2):140.  [Abstract]

    After 48 h of co-cultivation with 4-Octyl Itaconate (4-OI; 80 μM)-treated microglia, the proportion of apoptotic PC12 cells decreased significantly, the expression of Cleave-caspase3 decreased, and the expression of Bcl2 increased.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Free Radic Biol Med. 2022 Aug 1;188:404-418.

    Representative images showing immunofluorescent staining of Nrf2 (red) after valve interstitial cells are treated with 40 μM 4-Octyl Itaconate (OI) for 2 h.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Free Radic Biol Med. 2022 Aug 1;188:404-418.

    CCK-8 assay of the viability of valve interstitial cells treated with different concentrations of 4-Octyl Itaconate (OI) for 24 h.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Free Radic Biol Med. 2022 Aug 1;188:404-418.

    Relative quantification of the mRNA expression of Nrf2, Hmox1 and NQO1 after valve interstitial cells are treated with different concentrations of 4-Octyl Itaconate (OI) for 4 h.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Free Radic Biol Med. 2022 Aug 1;188:404-418.

    Representative Western blot images and quantification of Hmox1 and NQO1 protein expression after valve interstitial cells are treated with different concentrations of 4-Octyl Itaconate (OI) for 24 h.

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Int J Biol Sci. 2022; 18(16):6189-6209.

    Western blotting confirmes that TNF-α, IL-1β, and IFN-γ expression are significantly higher in RAW264.7 cells treated with LPS and are significantly inhibited by OI (4-Octyl Itaconate) (62.5, 125 µM; 12 h).

    4-Octyl Itaconate purchased from MCE. Usage Cited in: Front Pharmacol. 2021 May 25;12:651444.  [Abstract]

    Nrf2 knockdown attenuates the protective effect of 4-Octyl Itaconate (OI) in CCl4-treated NCTC 1469 cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    4-Octyl Itaconate is a cell-permeable Itaconate derivative. Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1[1].

    IC50 & Target

    Nrf2[1]
    In Vivo

    4-Octyl Itaconate (OI) is a cell-permeable itaconate derivative. 4-Octyl Itaconate is protective against lipopolysaccharide-induced lethality in vivo and decreases cytokine production[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    242.31

    Appearance

    Solid

    Formula

    C13H22O4
    CAS No.
    SMILES

    O=C(OCCCCCCCC)CC(C(O)=O)=C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (412.69 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.1269 mL 20.6347 mL 41.2694 mL
    5 mM 0.8254 mL 4.1269 mL 8.2539 mL
    10 mM 0.4127 mL 2.0635 mL 4.1269 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  20% HP-β-CD in saline

      Solubility: 5 mg/mL (20.63 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

    • 5.

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% saline

      Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

    • 6.

      Add each solvent one by one:  5% DMSO    95% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.98%

    COA (239 KB) LCMS (102 KB)

    Handling Instructions (2659 KB)
    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    4-Octyl Itaconate3133-16-2Keap1-Nrf2Inhibitorinhibitorinhibit

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