2. Academic Validation
  3. RNA-seq transcriptomic analysis of 4-octyl itaconate repressing myogenic differentiation

RNA-seq transcriptomic analysis of 4-octyl itaconate repressing myogenic differentiation

  • Arch Biochem Biophys. 2022 Sep 29;109420. doi: 10.1016/j.abb.2022.109420.
Lili Wang 1,  Zheng Chen 2,  Yu Feng 2,  Rongrong Wang 3,  Xiaohui Bai 4,  Weihua Liu 5,  Dawei Wang 6
Affiliations

Affiliations

  • 1 School of Nursing, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, 271000, Shandong, China.
  • 2 Department of Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, Shandong, China.
  • 3 Department of Clinical Laboratory, Shandong Provincial Hospital, Shandong University, Jinan, 250021, Shandong, China.
  • 4 Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, Shandong, China.
  • 5 School of Nursing, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, 271000, Shandong, China. Electronic address: [email protected].
  • 6 Department of Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, Shandong, China. Electronic address: [email protected].
PMID: 36183843 DOI: 10.1016/j.abb.2022.109420
Abstract

Background: The 4-octyl itaconate (OI) is a type of cell-permeable itaconate derivative. Studies have shown that with an anti-fibrotic effect in systemic sclerosis, the OI also affects osteoclast differentiation. The aim of this study was to explore the molecular mechanisms underlying the effects of OI on myoblast differentiation by RNA-seq analysis.

Methods: Myoblast proliferation, differentiation, and muscle regulatory factors were examined in C2C12 myoblasts treated with OI of various concentrations (2.5, 10, 25, 50, and 100 μmol/L). Cells were treated with the PI3K-Akt activator IGF-1 to explore the role of the PI3K-Akt pathway in OI inhibition of myogenic differentiation. The regulatory mechanisms of OI in myogenesis were further investigated by RNA-seq and subsequent gene ontology (GO), kyoto encyclopedia of genes and genomes (KEGG) and, gene set enrichment analysis (GSEA).

Results: OI of various concentrations did not show any effect during cell proliferation. During differentiation, OI inhibited the expressions of the marker of mature myotubes Myosin heavy chain (MHC) and myogenin in a dose-dependent manner. OI inhibited muscle differentiation by affecting MyoD-regulated activity through inhibition of Akt1 phosphorylation. The results of the KEGG enrichment analysis and GSEA showed that OI affected multiple metabolic pathways during myogenic differentiation, including PI3K-Akt signaling, calcium signaling, and PPAR signaling.

Conclusions: Our study broadens the understanding of the OI inhibition of myogenic differentiation. OI plays its functions by targeting multiple molecules and pathways, providing novel insights into the understanding of the overall effect of OI.

Keywords

-Akt1; 4-Octyl itaconate; MyoD; Myogenesis; Regeneration; p.

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