2. Metabolic Enzyme/Protease
  3. Lipase
  4. 4-Nitrophenyl palmitate

4-Nitrophenyl palmitate  (Synonyms: 4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp)

Cat. No.: HY-W013168 Pureza: 98.0%
COA
SDS
Instrucciones de manejo Technical Support

4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate) is a chromogenic substrate for lipases and esterases. Upon enzymatic hydrolysis, 4-Nitrophenyl palmitate releases p-nitrophenol, which can be quantified by colorimetric detection at 410 nm as a measure of enzymatic activity. 4-Nitrophenyl palmitate is used to characterize the activity of various bacterial and mammalian enzymes, including those from Burkholderia and porcine pancreatic lipase.

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4-Nitrophenyl palmitate

4-Nitrophenyl palmitate Estructura química

No. CAS : 1492-30-4

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
500 mg En stock
1 g En stock
5 g En stock
10 g En stock
25 g En stock
50 g En stock
100 g En stock
> 100 g   Obtener un presupuesto  

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4-Nitrophenyl palmitate Related Antibodies

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Descripciòn

4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate) is a chromogenic substrate for lipases and esterases. Upon enzymatic hydrolysis, 4-Nitrophenyl palmitate releases p-nitrophenol, which can be quantified by colorimetric detection at 410 nm as a measure of enzymatic activity. 4-Nitrophenyl palmitate is used to characterize the activity of various bacterial and mammalian enzymes, including those from Burkholderia and porcine pancreatic lipase[1][2].

In Vitro

4-Nitrophenyl palmitate serves as a substrate for measuring esterolytic activity of porcine pancreatic lipase, Staphylococcus aureus protease, and Burkholderia sp. lipase via colorimetric detection of released p-nitrophenol, with turbidity issues resolved by adding Triton X-100 to the reaction mixture[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Nitrophenyl palmitate Related Antibodies
Peso molecular

377.52

Fòrmula

C22H35NO4
No. CAS
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCCCC(OC1=CC=C([N+]([O-])=O)C=C1)=O
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad
In Vitro: 

Chloroform : ≥ 100 mg/mL (264.89 mM)

DMSO : 6.67 mg/mL (17.67 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6489 mL 13.2443 mL 26.4887 mL
5 mM 0.5298 mL 2.6489 mL 5.2977 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculadora de molaridad

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureza y Documentación

Purity: 98.0%

SDS (393 KB)

COA (240 KB) HNMR (118 KB)

Instrucciones de manejo (2659 KB)
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / Chloroform 1 mM 2.6489 mL 13.2443 mL 26.4887 mL 66.2217 mL
5 mM 0.5298 mL 2.6489 mL 5.2977 mL 13.2443 mL
10 mM 0.2649 mL 1.3244 mL 2.6489 mL 6.6222 mL
15 mM 0.1766 mL 0.8830 mL 1.7659 mL 4.4148 mL
Chloroform 20 mM 0.1324 mL 0.6622 mL 1.3244 mL 3.3111 mL
25 mM 0.1060 mL 0.5298 mL 1.0595 mL 2.6489 mL
30 mM 0.0883 mL 0.4415 mL 0.8830 mL 2.2074 mL
40 mM 0.0662 mL 0.3311 mL 0.6622 mL 1.6555 mL
50 mM 0.0530 mL 0.2649 mL 0.5298 mL 1.3244 mL
60 mM 0.0441 mL 0.2207 mL 0.4415 mL 1.1037 mL
80 mM 0.0331 mL 0.1656 mL 0.3311 mL 0.8278 mL
100 mM 0.0265 mL 0.1324 mL 0.2649 mL 0.6622 mL
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4-Nitrophenyl palmitate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrophenyl1492-30-44-Nitrophenyl hexadecanoatep-Nitrophenyl pNppLipaseesterasesPWTSBlipasesPWTSCBurkholderia sp.Staphylococcus aureusporcine pancreatic lipasepara-nitrophenolproteasesTriton X-100Inhibitorinhibitorinhibit

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Nombre del producto:
4-Nitrophenyl palmitate
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