4μ8C
Based on 74 publication(s) in Google Scholar
4μ8C (IRE1 Inhibitor III) is a small-molecule inhibitor of IRE1α.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 14003-96-4
- Formula: C11H8O4
- Molecular Weight:204.18
-
Storage:Powder -20°C, 3 years ; In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Cell Cycle/DNA Damage Compound Library
HY-L004
-
Kinase Inhibitor Library
HY-L009
-
Anti-Aging Compound Library
HY-L034
-
Covalent Screening Library
HY-L036
-
Endoplasmic Reticulum Stress Compound Library
HY-L054
-
Cysteine Targeted Covalent Library
HY-L153
-
Bioactive Compound Library Max
HY-L181
-
Anti-Aging Compound Library Mini
HY-L034M
-
Kinase Inhibitor Library Mini
HY-L009M
-
MCE Bioactive Compound Library
HY-L001V
-
Covalent Screening Library Plus
HY-L036P
-
Bioactive Compound Library
HY-L001
-
Protein Kinase Compound Library
HY-L196
-
High-Throughput Bioactive Compound Library
HY-L205
-
Posttranslational Modification Library
HY-L226
Publications Citing Use of MedChemExpress (MCE) 4μ8C
More- Signal Transduct Target Ther. 2024 Dec 30;9(1):367. [Abstract]
- Nature. 2023 Apr;616(7956):348-356. [Abstract]
- Immunity. 2025 Aug 22:S1074-7613(25)00332-2. [Abstract]
- Nat Metab. 2024 Aug;6(8):1601-1615. [Abstract]
- Nat Metab. 2022 Sep;4(9):1166-1184. [Abstract]
- Nat Cell Biol. 2023 May;25(5):726-739. [Abstract]
- Nat Commun. 2025 Dec 10. [Abstract]
- Nat Commun. 2024 May 15;15(1):4114. [Abstract]
- Exp Hematol Oncol. 2022 Mar 31;11(1):18. [Abstract]
- J Adv Res. 2026 Apr 1:S2090-1232(26)00273-0. [Abstract]
- Genes Dis. 2022 Dec 29;11(1):464-478. [Abstract]
- Acta Pharm Sin B. 2022 May;12(5):2315-2329. [Abstract]
- Cell Death Dis. 2026 Jun 3. [Abstract]
- Cell Death Dis. 2025 Jul 11;16(1):512. [Abstract]
- Cell Death Dis. 2025 Jan 14;16(1):12. [Abstract]
- Cell Mol Biol Lett. 2022 Dec 16;27(1):110. [Abstract]
- J Exp Med. 2022 Apr 4;219(4):e20211498. [Abstract]
- Phytomedicine. 2021 Jan;80:153399. [Abstract]
- J Pharm Anal. 2026 May 26.
- Cell Death Discov. 2023 Feb 17;9(1):69. [Abstract]
- Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):e2110476118. [Abstract]
- NPJ Breast Cancer. 2022 Jan 10;8(1):2. [Abstract]
- Free Radic Biol Med. 2026 Mar 21:S0891-5849(26)00242-X. [Abstract]
- Free Radic Biol Med. 2025 Dec 20:245:237-249. [Abstract]
- Free Radic Biol Med. 2025 Nov 8:243:414-433. [Abstract]
- ACS Appl Mater Interfaces. 2022 Jun 1;14(21):24144-24159. [Abstract]
- Cell Rep. 2026 Mar 17;45(4):117108. [Abstract]
- J Pineal Res. 2024 Aug;76(5):e12987. [Abstract]
- Food Sci Hum Wellness. 2026 Mar 3.
- Ecotoxicol Environ Saf. 2024 Jan 1:269:115782. [Abstract]
- Biochem Pharmacol. 2025 Jun 26:240:117089. [Abstract]
- Life Sci. 2024 Jul 1:348:122681. [Abstract]
- Acs Biomater Sci Eng. 2020 Mar 9;6(3):1727-1734. [Abstract]
- Front Microbiol. 2025 Sep 3:16:1648467. [Abstract]
- Mol Neurobiol. 2025 Nov 19;63(1):69. [Abstract]
- mBio. 2025 Dec 5:e0324225. [Abstract]
- mBio. 2025 Apr 16:e0060225. [Abstract]
- J Pathol. 2020 Jun;251(2):123-134. [Abstract]
- Toxicology. 2024 Aug 6:153906. [Abstract]
- Toxicology. 2024 May:504:153800. [Abstract]
- ACS Omega. 2021 Jun 8;6(24):15708-15715. [Abstract]
- Cell Signal. 2025 Jun:130:111702. [Abstract]
- Cell Signal. 2024 Dec 8:127:111560. [Abstract]
- J Dairy Sci. 2023 Jul;106(7):5146-5164. [Abstract]
- ACS Infect Dis. 2020 Jul 10;6(7):1745-1758. [Abstract]
- Front Cell Neurosci. 2021 Mar 25;15:614556. [Abstract]
- FASEB J. 2022 Jul;36(7):e22401. [Abstract]
- Fish Shellfish Immunol. 2024 Nov 9:155:110020. [Abstract]
- J Virol. 2024 Jun 21:e0081324. [Abstract]
- Toxins. 2020 Jan 16;12(1):55. [Abstract]
- Viruses. 2020 Sep 2;12(9):974. [Abstract]
- Cytokine. 2026 Jan:197:157068. [Abstract]
- Infect Immun. 2022 May 19;90(5):e0007922. [Abstract]
- Food Chem Toxicol. 2025 Jun 4:203:115592. [Abstract]
- Food Chem Toxicol. 2021 Jan;147:111911. [Abstract]
- Fish Physiol Biochem. 2024 Dec;50(6):2287-2299. [Abstract]
- Mol Biol Cell. 2024 Jun 1;35(6):br12. [Abstract]
- Theriogenology. 2022 May 16;187:205-214. [Abstract]
- Vet Microbiol. 2026 May:316:110968. [Abstract]
- Toxicon. 2021 Sep:200:165-172. [Abstract]
- Vet Microbiol. 2020 May;244:108648. [Abstract]
- Biochem Biophys Res Commun. 2020 Jun 30;527(3):805-810. [Abstract]
- Curr Med Sci. 2023 Aug;43(4):689-695. [Abstract]
- Mol Cell Toxicol. 2025 Jul 18.
- Bio Protoc. 2025 Aug 20;15(16):e5414. [Abstract]
- bioRxiv. 2026 May 29:2026.05.26.727520. [Abstract]
- bioRxiv. 2026 May 31.
- bioRxiv. 2026 Feb 24.
- bioRxiv. 2025 Aug 08.
- bioRxiv. 2025 February 10.
- bioRxiv. 2024 September 30.
- Research Square Preprint. 2024 Mar 15.
- Environ Toxicol. 2023 Jul;38(7):1756-1767. [Abstract]
- Oxid Med Cell Longev. 2021 Jan 4;2021:8836818. [Abstract]
Customer Validation & Images
-
2D/3D Cell Culture and Differentiation
-
RT-PCR
-
RT-PCR
-
RT-PCR
-
WB
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| JeKo-1 | IC50 |
1.57 μM
Compound: 5, 4mu8C
|
Inhibition of XBP-1s expression in human Jeko cells after 24 hrs by immunoblotting analysis
Inhibition of XBP-1s expression in human Jeko cells after 24 hrs by immunoblotting analysis
|
[PMID: 24749861] |
| Sf21 | IC50 |
206 nM
Compound: 5, 4mu8C
|
Inhibition of human recombinant puritin-His-tagged IRE-1 RNase expressed in SF21 cells using XBP-1 RNA stem loop as substrate incubated for 30 mins prior to substrate addition measured after 2 hrs by FRET-suppression assay
Inhibition of human recombinant puritin-His-tagged IRE-1 RNase expressed in SF21 cells using XBP-1 RNA stem loop as substrate incubated for 30 mins prior to substrate addition measured after 2 hrs by FRET-suppression assay
|
[PMID: 24749861] |
When applies to the media of ER stressed cultured cells, 4μ8C (IRE1 Inhibitor III) inhibits Xbp1 splicing in a concentration-dependent manner. 4μ8C dissociates slowly from IRE1, but ishout of inhibitor leads to rapid recovery of Xbp1 splicing in cells[1].The IRE1 endoribonuclease inhibitor 4μ8c prevents the splicing of the XBP1 mRNA in response to ER stress caused by mutant proinsulin production[2]. The inositol-requiring enzyme 1α (IRE1α) is a serine-threonine kinase that plays crucial roles in activating the unfolded protein response. 4μ8C treatment dramatically inhibits IL-4 production by CD4+ T cells under Th0 conditions because both the IL-4 levels in the culture supernatant and the percentage of IL-4 positive cells are reduced by 4μ8C treatment. In addition, both IL-5 and IL-13 production are significantly reduced upon treatment with 4μ8C[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
4μ8C (orally; 10, 50, or 100 mg/kg) suppresses passive cutaneous anaphylaxis (PCA) in mice (ED50 = 25.1 mg/kg)[5].
4μ8C reverses the ER stress-dependent loss of several known RIDD targets, with an EC50 of approximately 4 μM, approximating that of inhibition of XBP1 target gene activation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:ApoE-/- mice[4]
-
Dosage:10 mg/kg
-
Administration:I.p. injection; daily; for 4 more weeks
-
Result:Led to a significant reduction (45.2%) in atherosclerotic lesion area in en face aorta preparations.
Chemical Information
-
CAS No. 14003-96-4
-
Appearance Solid
-
Molecular Weight 204.18
-
Formula C11H8O4
-
Color Light yellow to yellow
-
SMILES
O=CC1=C(O)C=CC2=C1OC(C=C2C)=O
-
Synonyms
IRE1 Inhibitor III
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years In solvent -80°C 1 year -20°C 6 months
Publications (74)
-
Journal Impact Factor
-
Most Recent
-
Signal Transduct Target Ther
2024 Dec 30;9(1):367. PMID: 39737965 -
Nature
2023 Apr;616(7956):348-356. PMID: 37020026 -
Immunity
Unfolded protein response transcription factor XBP1 suppresses necroptosis-induced colitis by reinforcing the mucus barrier. [Abstract]2025 Aug 22:S1074-7613(25)00332-2. PMID: 40865520
4μ8C purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Aug 22:S1074-7613(25)00332-2. [Abstract]
Representative images of mouse colon organoids treated with G'9668 or 4μ8C (10 μM) for 24 h after being differentiated with DAPT for 5 days. Scale bars, 360 μm.
4μ8C purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Aug 22:S1074-7613(25)00332-2. [Abstract]
Graphs depicting relative mRNA expression of Muc2 in colon organoids treated with G'9668 or 4μ8C (10 μM) for 24 h after incubation, with or without DAPT for 5 days, measured by RT-qPCR.
4μ8C purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Aug 22:S1074-7613(25)00332-2. [Abstract]
Graphs depicting relative mRNA expression of Agr2 in colon organoids treated with G'9668 or 4μ8C (10 μM) for 24 h after incubation, with or without DAPT for 5 days, measured by RT-qPCR.
-
Nat Metab
Hydrogen sulfide produced by the gut microbiota impairs host metabolism via reducing GLP-1 levels in male mice. [Abstract]2024 Aug;6(8):1601-1615. PMID: 39030389
4μ8C purchased from MedChemExpress. Usage Cited in: Nat Metab. 2024 Aug;6(8):1601-1615. [Abstract]
Xbp1 splicing and Gcg expression in NaHS-treated cells in the presence of 60 μM 4μ8c for 2h (n = 4 for all groups).
-
Nat Metab
2022 Sep;4(9):1166-1184. PMID: 36123394 -
Nat Cell Biol
SEL1L-HRD1 endoplasmic reticulum-associated degradation controls STING-mediated innate immunity by limiting the size of the activable STING pool. [Abstract]2023 May;25(5):726-739. PMID: 37142791 -
Nat Commun
Ubiquitination by HRD1 is essential for TLR3 trafficking and its innate immune signaling. [Abstract]2025 Dec 10. PMID: 41372171 -
Nat Commun
2024 May 15;15(1):4114. PMID: 38750057 -
Exp Hematol Oncol
RNA sequencing identifies novel regulated IRE1-dependent decay targets that affect multiple myeloma survival and proliferation. [Abstract]2022 Mar 31;11(1):18. PMID: 35361260 -
J Adv Res
Rutin triggers IRE1-mediated GSDMD-dependent pyroptosis in macrophages to suppress systemic Salmonella infection. [Abstract]2026 Apr 1:S2090-1232(26)00273-0. PMID: 41932494 -
Genes Dis
IRE1α regulates the PTHrP-IHH feedback loop to orchestrate chondrocyte hypertrophy and cartilage mineralization. [Abstract]2022 Dec 29;11(1):464-478. PMID: 37588212 -
Acta Pharm Sin B
The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1 α/XBP1s pathway. [Abstract]2022 May;12(5):2315-2329. PMID: 35646520 -
Cell Death Dis
The OAS1-FASN axis promotes pancreatic cancer by coordinating lipogenic stress and the unfolded protein response. [Abstract]2026 Jun 3. PMID: 42236684 -
Cell Death Dis
IFNγ augments TKI efficacy by alleviating protein unfolding stress to promote GSDME-mediated pyroptosis in hepatocellular carcinoma. [Abstract]2025 Jul 11;16(1):512. PMID: 40645933 -
Cell Death Dis
Involvement of p38 MAPK and MAPKAPK2 in promoting cell death and the inflammatory response to ischemic stress associated with necrotic glioblastoma. [Abstract]2025 Jan 14;16(1):12. PMID: 39805854 -
Cell Mol Biol Lett
Metabolic disorders sensitise endometrial carcinoma through endoplasmic reticulum stress. [Abstract]2022 Dec 16;27(1):110. PMID: 36526973 -
J Exp Med
PERK reprograms hematopoietic progenitor cells to direct tumor-promoting myelopoiesis in the spleen. [Abstract]2022 Apr 4;219(4):e20211498. PMID: 35266960 -
Phytomedicine
Effect of pristimerin on apoptosis through activation of ROS/ endoplasmic reticulum (ER) stress-mediated noxa in colorectal cancer. [Abstract]2021 Jan;80:153399. PMID: 33202325 -
-
Cell Death Discov
XBP1 modulates endoplasmic reticulum and mitochondria crosstalk via regulating NLRP3 in renal ischemia/reperfusion injury. [Abstract]2023 Feb 17;9(1):69. PMID: 36801911 -
Proc Natl Acad Sci U S A
2021 Sep 28;118(39):e2110476118. PMID: 34544877 -
NPJ Breast Cancer
Endoplasmic reticulum stress inhibits AR expression via the PERK/eIF2α/ATF4 pathway in luminal androgen receptor triple-negative breast cancer and prostate cancer. [Abstract]2022 Jan 10;8(1):2. PMID: 35013318 -
Free Radic Biol Med
Cannabigerol induces endoplasmic reticulum stress-mediated apoptosis and ferroptosis via the IRE1α-XBP1 axis in human pancreatic cancer cells. [Abstract]2026 Mar 21:S0891-5849(26)00242-X. PMID: 41871732 -
Free Radic Biol Med
TRAF6 mediates vascular remodeling via endoplasmic reticulum stress-mitophagy in hypoxic pulmonary hypertension. [Abstract]2025 Dec 20:245:237-249. PMID: 41429276 -
Free Radic Biol Med
TRIB2 promotes pulmonary artery smooth muscle cell proliferation through SERCA2 ubiquitination in pulmonary hypertension. [Abstract]2025 Nov 8:243:414-433. PMID: 41213438 -
ACS Appl Mater Interfaces
Polyaniline-Based Glyco-Condensation on Au Nanoparticles Enhances Immunotherapy in Lung Cancer. [Abstract]2022 Jun 1;14(21):24144-24159. PMID: 35579575 -
Cell Rep
ALG6 orchestrates coronavirus replication via the endoplasmic reticulum stress-autophagy axis. [Abstract]2026 Mar 17;45(4):117108. PMID: 41855202 -
J Pineal Res
2024 Aug;76(5):e12987. PMID: 38975671 -
-
Ecotoxicol Environ Saf
2024 Jan 1:269:115782. PMID: 38056121 -
Biochem Pharmacol
Ghrelin attenuates metabolic dysfunction-associated steatotic liver disease by activating glutamine metabolism via inhibition of the IRE1α-XBP-1 pathway. [Abstract]2025 Jun 26:240:117089. PMID: 40581331 -
Life Sci
GRP78 blockade overcomes acquired resistance to EGFR-tyrosine kinase inhibitors in non-small cell lung cancer. [Abstract]2024 Jul 1:348:122681. PMID: 38697281 -
Acs Biomater Sci Eng
Multifunctional Single-Layered Graphene Quantum Dots Used for Diagnosis of Mitochondrial Malfunction-Related Diseases. [Abstract]2020 Mar 9;6(3):1727-1734. PMID: 33455364 -
Front Microbiol
Ire1 inhibitors attenuate Candida albicans pathogenicity and demonstrate potential for application in antifungal therapy. [Abstract]2025 Sep 3:16:1648467. PMID: 40969430 -
Mol Neurobiol
CK2α Regulates Endoplasmic Reticulum Stress-Mediated Mitophagy via the PERK/ATF4/CHOP Pathway in Rotenone-Treated SH-SY5Y Cells. [Abstract]2025 Nov 19;63(1):69. PMID: 41254351 -
mBio
Quantitative proteomic analysis identifies the unfolded protein response as a host pathway co-opted by ASFV to promote replication. [Abstract]2025 Dec 5:e0324225. PMID: 41347792 -
mBio
AUP1 and UBE2G2 complex targets STING signaling and regulates virus-induced innate immunity. [Abstract]2025 Apr 16:e0060225. PMID: 40237449 -
J Pathol
Function and mutual interaction of BiP-, PERK-, and IRE1α-dependent signalling pathways in vascular tumours. [Abstract]2020 Jun;251(2):123-134. PMID: 32166747 -
Toxicology
Proteasome inhibition induces apoptosis through simultaneous inactivation of MCL-1/BCL-XL by NOXA independent of CHOP and JNK pathways. [Abstract]2024 Aug 6:153906. PMID: 39117261 -
Toxicology
Differential susceptibility of BRL cells with/without insulin resistance and the role of endoplasmic reticulum stress signaling pathway in response to acrylamide-exposure toxicity effects in vitro. [Abstract]2024 May:504:153800. PMID: 38604440 -
ACS Omega
Combination of Palmitic Acid and Methylseleninic Acid Induces Mitochondria-Dependent Apoptosis via Attenuation of the IRE1α Arm and Enhancement of CHOP in Hepatoma. [Abstract]2021 Jun 8;6(24):15708-15715. PMID: 34179614 -
Cell Signal
Unfolded protein response-activated NLRP3 inflammasome contributes to pyroptotic and apoptotic podocyte injury in diabetic kidney disease via the CHOP-TXNIP axis. [Abstract]2025 Jun:130:111702. PMID: 40020889 -
Cell Signal
Inhibition of ERO1L induces autophagy and apoptosis via endoplasmic reticulum stress in colorectal cancer. [Abstract]2024 Dec 8:127:111560. PMID: 39657838 -
J Dairy Sci
AMPK-endoplasmic reticulum stress axis contributes to lipopolysaccharide-caused mitochondrial dysfunction by regulating mitochondria-associated membrane function in bovine hepatocytes. [Abstract]2023 Jul;106(7):5146-5164. PMID: 37225589 -
ACS Infect Dis
Targeting the Inositol-Requiring Enzyme-1 Pathway Efficiently Reverts Zika Virus-Induced Neurogenesis and Spermatogenesis Marker Perturbations. [Abstract]2020 Jul 10;6(7):1745-1758. PMID: 32329611 -
Front Cell Neurosci
IRE1α-XBP1 Affects the Mitochondrial Function of Aβ25-35-Treated SH-SY5Y Cells by Regulating Mitochondria-Associated Endoplasmic Reticulum Membranes. [Abstract]2021 Mar 25;15:614556. PMID: 33841100 -
FASEB J
SNAI1 is upregulated during muscle regeneration and represses FGF21 and ATF3 expression by directly binding their promoters. [Abstract]2022 Jul;36(7):e22401. PMID: 35726676 -
Fish Shellfish Immunol
Grass Carp Reovirus (GCRV) infection activates the PERK-eIF2α pathway to promote the viral replication. [Abstract]2024 Nov 9:155:110020. PMID: 39528019 -
J Virol
Enterovirus 3A protein disrupts endoplasmic reticulum homeostasis through interaction with GBF1. [Abstract]2024 Jun 21:e0081324. PMID: 38904364 -
Toxins
Activation of the IRE1α Arm, but not the PERK Arm, of the Unfolded Protein Response Contributes to Fumonisin B1-Induced Hepatotoxicity. [Abstract]2020 Jan 16;12(1):55. PMID: 31963346
4μ8C purchased from MedChemExpress. Usage Cited in: Toxins. 2020 Jan 16;12(1):55. [Abstract]
The effect of the IRE1 specific inhibitor 4μ8C on the expression of apoptosis-related proteins. The cells are exposed to FB1 with or without 4μ8C for 24 h, and the phosphorylation of JNK, Mcl-1, Bak, Bax, and Puma were analyzed by Western blotting. n = 3.
-
Viruses
2020 Sep 2;12(9):974. PMID: 32887282 -
Cytokine
Triptolide impacts CSF1R expression and reprograms the suppressive function of myeloid-derived suppressor cells via targeting the ER stress pathway. [Abstract]2026 Jan:197:157068. PMID: 41197245 -
Infect Immun
Chlamydia psittaci Induces Autophagy in Human Bronchial Epithelial Cells via PERK and IRE1α, but Not ATF6 Pathway. [Abstract]2022 May 19;90(5):e0007922. PMID: 35435728 -
Food Chem Toxicol
Mechanisms of aristolochic acid I Hepatotoxicity: Central role of PDK4-Induced mitochondrial dysfunction and hepatic inflammation. [Abstract]2025 Jun 4:203:115592. PMID: 40480416 -
Food Chem Toxicol
IRE1 and CaMKKβ pathways to reveal the mechanism involved in microcystin-LR-induced autophagy in mouse ovarian cells. [Abstract]2021 Jan;147:111911. PMID: 33290805 -
Fish Physiol Biochem
Regulation of lipid metabolism in grass carp primary hepatocytes by exosomes derived from fatty hepatocytes though GRP78. [Abstract]2024 Dec;50(6):2287-2299. PMID: 39090453 -
Mol Biol Cell
Baseline Unfolded Protein Response Signaling Adjusts the Timing of the Mammalian Cell Cycle. [Abstract]2024 Jun 1;35(6):br12. PMID: 38656789 -
Theriogenology
Mito-Q promotes porcine oocytes maturation by maintaining mitochondrial thermogenesis via UCP2 downregulation. [Abstract]2022 May 16;187:205-214. PMID: 35644089 -
Vet Microbiol
The PERK-eIF2α branch activates the NLRP3 inflammasome through the NF-κB signaling pathway to suppress NDV replication. [Abstract]2026 May:316:110968. PMID: 41793984 -
Toxicon
Glycyrol alleviates the combined toxicity of fumonisin B1 and cadmium in vitro and in vivo. [Abstract]2021 Sep:200:165-172. PMID: 34343521 -
Vet Microbiol
A rough Brucella mutant induced macrophage death depends on secretion activity of T4SS, but not on cellular Txnip- and Caspase-2-mediated signaling pathway. [Abstract]2020 May;244:108648. PMID: 32402333 -
Biochem Biophys Res Commun
Chlamydia trachomatis plasmid-encoded protein pORF5 activates unfolded protein response to induce autophagy via MAPK/ERK signaling pathway. [Abstract]2020 Jun 30;527(3):805-810. PMID: 32446560 -
Curr Med Sci
Hypoxia Affects Autophagy in Human Umbilical Vein Endothelial Cells via the IRE1 Unfolded Protein Response. [Abstract]2023 Aug;43(4):689-695. PMID: 37558862 -
-
Bio Protoc
An Ex Vivo Protocol to Assess IRE1α-Dependent RNA Cleavage Using Total RNA Isolated from Mouse Tissues. [Abstract]2025 Aug 20;15(16):e5414. PMID: 40901563 -
bioRxiv
2026 May 29:2026.05.26.727520. PMID: 42244578 -
-
-
-
-
-
-
Environ Toxicol
Endoplasmic reticulum chaperone GRP78 participates in fluoride-induced autophagy in LS8 cells by regulating the IRE1-TRAF2-JNK pathway. [Abstract]2023 Jul;38(7):1756-1767. PMID: 37070943
4μ8C purchased from MedChemExpress. Usage Cited in: Environ Toxicol. 2023 Jul;38(7):1756-1767. [Abstract]
4μ8C (20 μM; 1 h) significantly inhibits NaF-induced increased expression of p-IRE1 , GRP78 , p-TRAF2 , p-JNK, and LC3II/I in LS8 cells.
-
Oxid Med Cell Longev
Stimulation of Sigma-1 Receptor Protects against Cardiac Fibrosis by Alleviating IRE1 Pathway and Autophagy Impairment. [Abstract]2021 Jan 4;2021:8836818. PMID: 33488945
Solvent & Solubility
DMSO : 33.33 mg/mL (163.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 25 mg/mL (122.44 mM); Suspended solution; Need ultrasonic
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Cross BC, et al. The molecular basis for selective inhibition of unconventional mRNA splicing by an IRE1-binding small molecule. Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):E869-78. [Content Brief]
[2]. Zhang L, et al. IRE1 inhibition perturbs the unfolded protein response in a pancreatic β-cell line expressing mutant proinsulin, but does not sensitize the cells to apoptosis. BMC Cell Biol. 2014 Jul 10;15:29. [Content Brief]
[4]. Tufanli O, et al. Targeting IRE1 with small molecules counteracts progression of atherosclerosis. Proc Natl Acad Sci U S A. 2017 Feb 21;114(8):E1395-E1404. [Content Brief]
[5]. Nam ST, et al. Suppression of IgE-mediated mast cell activation and mouse anaphylaxis via inhibition of Sykactivation by 8-formyl-7-hydroxy-4-methylcoumarin, 4μ8C. Toxicol Appl Pharmacol. 2017 Oct 1;332:25-31. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.8976 mL | 24.4882 mL | 48.9764 mL | 122.4410 mL |
| 5 mM | 0.9795 mL | 4.8976 mL | 9.7953 mL | 24.4882 mL | |
| 10 mM | 0.4898 mL | 2.4488 mL | 4.8976 mL | 12.2441 mL | |
| 15 mM | 0.3265 mL | 1.6325 mL | 3.2651 mL | 8.1627 mL | |
| 20 mM | 0.2449 mL | 1.2244 mL | 2.4488 mL | 6.1220 mL | |
| 25 mM | 0.1959 mL | 0.9795 mL | 1.9591 mL | 4.8976 mL | |
| 30 mM | 0.1633 mL | 0.8163 mL | 1.6325 mL | 4.0814 mL | |
| 40 mM | 0.1224 mL | 0.6122 mL | 1.2244 mL | 3.0610 mL | |
| 50 mM | 0.0980 mL | 0.4898 mL | 0.9795 mL | 2.4488 mL | |
| 60 mM | 0.0816 mL | 0.4081 mL | 0.8163 mL | 2.0407 mL | |
| 80 mM | 0.0612 mL | 0.3061 mL | 0.6122 mL | 1.5305 mL | |
| 100 mM | 0.0490 mL | 0.2449 mL | 0.4898 mL | 1.2244 mL |
4μ8C Related Classifications
HY-19707 Related Classifications
Powered by Bioz