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  4. Bromobimane

Bromobimane  (Synonyms: Monobromobimane; ブロモビマン)

製品番号: HY-100041 純度: 99.31%
COA 取扱説明書 Technical Support

Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols.

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Bromobimane

Bromobimane 構造式

CAS 番号 : 71418-44-5

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in DMSO USD 33 在庫あり
Solid
5 mg $30 在庫あり
10 mg $50 在庫あり
25 mg $105 在庫あり
50 mg $180 在庫あり
100 mg $300 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 8 publication(s) in Google Scholar

Other Forms of Bromobimane:

Bromobimane 関連抗体

Top Publications Citing Use of Products

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols[1][2].

体外実験

Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
Bromobimane (mBBr) labeled reactive thiols in cells[1]
(1) Prepare a 100 mM mBBr solution with acetonitrile.
(2) Add 1 mL of 10% cell suspension to 15-25 μL of 100 mM mBBr solution.
(3) After incubation at 37°C for 30-45 min, fluorescence detection was performed.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bromobimane 関連抗体
分子量

271.11

分子式

C10H11BrN2O2
Emission (Em)

450
Excitation (Ex)

344
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1N(C(C(C)=C2C)=O)N2C(CBr)=C1C
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : 87.5 mg/mL (322.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 1 mg/mL (3.69 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6885 mL 18.4427 mL 36.8854 mL
5 mM 0.7377 mL 3.6885 mL 7.3771 mL
10 mM 0.3689 mL 1.8443 mL 3.6885 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
純度とドキュメンテーション

純度: 99.31%

COA (255 KB) LCMS (95 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.6885 mL 18.4427 mL 36.8854 mL 92.2135 mL
DMSO 5 mM 0.7377 mL 3.6885 mL 7.3771 mL 18.4427 mL
10 mM 0.3689 mL 1.8443 mL 3.6885 mL 9.2213 mL
15 mM 0.2459 mL 1.2295 mL 2.4590 mL 6.1476 mL
20 mM 0.1844 mL 0.9221 mL 1.8443 mL 4.6107 mL
25 mM 0.1475 mL 0.7377 mL 1.4754 mL 3.6885 mL
30 mM 0.1230 mL 0.6148 mL 1.2295 mL 3.0738 mL
40 mM 0.0922 mL 0.4611 mL 0.9221 mL 2.3053 mL
50 mM 0.0738 mL 0.3689 mL 0.7377 mL 1.8443 mL
60 mM 0.0615 mL 0.3074 mL 0.6148 mL 1.5369 mL
80 mM 0.0461 mL 0.2305 mL 0.4611 mL 1.1527 mL
100 mM 0.0369 mL 0.1844 mL 0.3689 mL 0.9221 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Bromobimane Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bromobimane71418-44-5MonobromobimaneFluorescent DyeInhibitorinhibitorinhibit

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