1. NF-κB
  2. Keap1-Nrf2

CDDO-EA (Synonyms: CDDO ethyl amide; TP319; RTA 405)

Cat. No.: HY-12213 Purity: 99.32%
Handling Instructions

CDDO-EA is an NF-E2 related factor 2/antioxidant response element (Nrf2/ARE) activator.

For research use only. We do not sell to patients.

CDDO-EA Chemical Structure

CDDO-EA Chemical Structure

CAS No. : 932730-51-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 479 In-stock
Estimated Time of Arrival: December 31
5 mg USD 420 In-stock
Estimated Time of Arrival: December 31
10 mg USD 600 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1800 In-stock
Estimated Time of Arrival: December 31
100 mg USD 2520 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

CDDO-EA is an NF-E2 related factor 2/antioxidant response element (Nrf2/ARE) activator.

IC50 & Target

Nrf2/ARE[1]

In Vitro

CDDO-EA potently activates Nrf2/ARE in a cell culture model of ALS and in the G93A SOD1 mouse model of ALS[1]. CDDO-EA is a potent inducer of apoptosis in A549 lung cancer cells, as shown both by PARP cleavage and Annexin staining. CDDO-EA is more potent than CDDO itself as inducers of heme oxygenase-1 (HO-1). In RAW264.7 macrophage-like cells, CDDO-EA is 7-fold more potent than CDDO as suppressors of the ability of IFN-γ to induce iNOS[2].

In Vivo

The survival analysis shows that G93A mice treated with CDDO-EA, compared to G93A littermate controls, lives significantly longer. CDDO-EA treatment increases the life-span by 20.6 days from 124.05±3.7 days to 144.72±8.1 days (16.6%) (p<0.001). In CDDO-EA-treated G93A mice, the age of death is 141.4±5.2 days and the duration from the age of onset to the age of death is 57.6±7.6 days, which means that the age of death from onset is prolonged by 17.5 days (43%)[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9278 mL 9.6389 mL 19.2779 mL
5 mM 0.3856 mL 1.9278 mL 3.8556 mL
10 mM 0.1928 mL 0.9639 mL 1.9278 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Wild-type and Nrf2−/− mouse embryonic fibroblasts are pre-treated with CDDO-EA or CDDO-TFEA at various concentrations (1, 10 and 100 nM in DMSO) for 18 hours and incubated with 2’,7’-Dichlorodihydrofluorecein diacetate (H2DCFDA) for 30 min. Cells are challenged with 250 µM tBHP for 15-30 min and the mean fluorescence intensity for ~10,000 cells is analyzed by FACSan flow cytometry using a 480-nm excitation wavelength and a 525-nm emission wavelength[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]
G93A SOD1 transgenic familial ALS mice (high copy number) B6SJL background strain (G93A SOD1, B6SJL-TgGur1) are used. G93A transgenic mice are assigned randomly to the control (vehicle, mouse chaw only) and to mouse chaw containing either CDDO-EA or CDDO-TFEA (400 mg/kg of food, n=30 in both groups). This dose corresponds to about 80 mg/kg body weight/day, assuming each mouse consumes 5 grams of food per day. We found mice can tolerate this dose. Treatments started at two different time regimens: 1) “Early” at 30 days of age, about two months prior to symptom onset; 2) “At Onset” from the onset of the phenotype (80-90 days of age). A diet consisting of either 400 mg of CDDO-TFEA per kg of food or 400 mg of CDDO-EA per kg of food, and a control lab diet, are prepared by Purina.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

518.73

Formula

C₃₃H₄₆N₂O₃

CAS No.

932730-51-3

SMILES

O=C1C(C#N)=C[[email protected]@]2(C)[[email protected]](CC[[email protected]]([[email protected]@]3(C)[[email protected]@]4([H])[[email protected]@]5([H])[[email protected]@](CCC(C)(C)C5)(C(NCC)=O)CC3)(C)C2=CC4=O)([H])C1(C)C

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
CDDO-EA
Cat. No.:
HY-12213
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CDDO-EA

Cat. No.: HY-12213