1. Cell Cycle/DNA Damage
    Antibody-drug Conjugate/ADC Related
  2. DNA Alkylator/Crosslinker
    ADC Cytotoxin
  3. Calicheamicin

Calicheamicin (Synonyms: Calicheamicin γ1)

Cat. No.: HY-19609 Purity: 98.44%
Handling Instructions

Calicheamicin, an antitumor antibiotic, is a cytotoxic agent that causes double-strand DNA breaks. Calicheamicin is a DNA synthesis inhibitor.

For research use only. We do not sell to patients.

Calicheamicin Chemical Structure

Calicheamicin Chemical Structure

CAS No. : 108212-75-5

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 2709 In-stock
Estimated Time of Arrival: December 31
1 mg USD 480 In-stock
Estimated Time of Arrival: December 31
5 mg USD 1020 In-stock
Estimated Time of Arrival: December 31
10 mg USD 1800 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Calicheamicin:

Top Publications Citing Use of Products

    Calicheamicin purchased from MCE. Usage Cited in: FASEB J. 2018 Jun 6:fj201800092R.

    The distribution of PARP-1 is compared in the 4 fractions after Neocarzinostatin (NCS) or Calicheamicin (Cali) treatment in Ku70-deficient and Ku70-normal MDA-MB-231 and BT-549 cells.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review


    Calicheamicin, an antitumor antibiotic, is a cytotoxic agent that causes double-strand DNA breaks. Calicheamicin is a DNA synthesis inhibitor[1].

    IC50 & Target



    In Vitro

    PF-06647263 (anti-EFNA4-ADC) is generated via conjugation of hE22 lysine residues to the AcButDMH-N-Ac-calicheamicin-γ1 linker-payload with an average drug-to-antibody ratio (DAR) of 4.6. PF-06647263 elicits antigen- and concentration-dependent cytotoxicity, as exposure to PF-06647263 for 96 hours results in cell death (EC50= appr 1 ng/mL)[1]. CMC-544, consisting of a humanized CD22 Ab linked to calicheamicin, is effective in pediatric primary B-cell precursor acute lymphoblastic leukemia (BCP-ALL) cells in vitro. CMC-544 induces cell death in various ALL cell lines in a dose- and time-dependent way, with IC50 values ranging from 0.15 to 4.9 ng/mL. CMC-544 (10 ng/mL) is effective and specific in primary BCP-ALL cells[2]. In CMC-544-treated cells, the level of CD22 has decreased relative to that on G5/44-treated cells and continued to decrease[3].

    In Vivo

    An ADC comprising a humanized anti-EFNA4 monoclonal antibody conjugated to the DNA-damaging agent calicheamicin achieves sustained tumor regressions in both TNBC and ovarian cancer PDX in vivo. PF-06647263 (0.27, 0.36 mg/kg) results in significant tumor regressions in TNBC xenografts[1].

    Molecular Weight




    CAS No.



    Room temperature in continental US; may vary elsewhere.

    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (73.08 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.7308 mL 3.6540 mL 7.3081 mL
    5 mM 0.1462 mL 0.7308 mL 1.4616 mL
    10 mM 0.0731 mL 0.3654 mL 0.7308 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 3 mg/mL (2.19 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 3 mg/mL (2.19 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    Cell Assay

    The enhancement of the CDC effect is studied in a similar way in the presence of CMC-544 or G5/44. Specifically, after cells are incubated with or without CMC-544 (5 ng/mL calichemicin DMH) or G5/44 at 37°C for 2 h, they are washed three times to remove unbound antibodies. The viability of cells before incubation with CMC-544 is 99.8%. After the cells are re-incubated in CMC-544- and rituximab-free medium at 37°C for 0-48 h, CDC is analyzed.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration

    Cohorts of tumor-bearing mice (140-180 mm3) are randomized into study groups of 6 to 10 based on the number of available mice. The IDBS electronic notebook statistical package, Biobook, is used for automated animal randomization. Animals are dosed by intraperitoneal injection (or intravenously for 144580) twice a week for 4 cycles with ADC, or once a week for 2 cycles with 1.5 mg/kg doxorubicin for breast PDX tumors or 5 mg/kg Cisplatin for ovarian PDX. Study groups are followed until either individual mice or entire cohort measurements reach 1,200 mm3, at which point sacrifice is indicated. Tumor regression is defined as a reduction in mean tumor volume after dosing. In cases where tumors regress, time to progression (TTP) is determined to be the number of days between the first dose and the time at which mean tumor volume significantly increase (regrow) after regression.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.


    Purity: 98.44%

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    CalicheamicinCalicheamicin γ1Calicheamicin γ 1Calicheamicin γ-1DNA Alkylator/CrosslinkerADC CytotoxinBacterialApoptosisAntibioticInhibitorinhibitorinhibit

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