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  4. Congo Red

Congo Red is an azo dye. Congo Red (CR) binding been used as a diagnostic test for the presence of amyloid in tissue sections.

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Congo Red

Congo Red Chemische Struktur

CAS. Nr. : 573-58-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 Auf Lager
Solution
10 mM * 1 mL in DMSO Auf Lager
Solid
500 mg Auf Lager
1 g Auf Lager
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10 g   Angebot einholen  

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Based on 4 publication(s) in Google Scholar

Other Forms of Congo Red:

Congo Red Related Antibodies

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Beschreibung

Congo Red is an azo dye. Congo Red (CR) binding been used as a diagnostic test for the presence of amyloid in tissue sections.

Cellular Effect
Cell Line Type Value Description References
PC-12 ED50
37.5 6
Compound: 10
Protection against beta-amyloid (25 to 35) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
Protection against beta-amyloid (25 to 35) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
[PMID: 12350137]
PC-12 ED50
39.2 6
Compound: 10
Protection against beta-amyloid (1 to 42) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
Protection against beta-amyloid (1 to 42) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
[PMID: 12350137]
In Vitro

Congo Red histochemical stain may serve as a simple screening tool for investigating if the aggregates in mutant cells have misfolded β-pleated sheet secondary structures. Congo Red histochemical dye has the ability to bind specifically to crossed β-pleated sheet structures. Wild-type HSPB1 should maintain protein homeostasis by binding proteins in non-native conformations, thereby preventing substrate aggregation. The T139M mutant, however, fails in this function and results in an accumulation of misfolded proteins, which are targeted by Congo Red for intercalating between the β-pleated sheet structures. Congo Red histochemical stain may serve as a simple tool to investigate if the aggregates in mutant cells have misfolded β-pleated sheet secondary structures[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Congo Red Related Antibodies
Klinische Studie
Molekulargewicht

696.66

Formel

C32H22N6Na2O6S2
CAS. Nr.
Appearance

Solid

Color

Pink to red

SMILES

O=S(C1=C2C=CC=CC2=C(N)C(N=NC3=CC=C(C4=CC=C(N=NC5=CC(S(=O)(O[Na])=O)=C6C=CC=CC6=C5N)C=C4)C=C3)=C1)(O[Na])=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 20 mg/mL (28.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4354 mL 7.1771 mL 14.3542 mL
5 mM 0.2871 mL 1.4354 mL 2.8708 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Reinheit & Dokumentation
Verweise
Zellassay
[1]

HeLa cells are selected for these studies due to their large cytoplasmic volume. Cells transfected with mutant or wild-type HSPB1 constructs are grown on coverslips for 24 hr and then are stained with Congo red to determine if the aggregates display amyloidogenic properties. Briefly, cells are first fixed with 10% formalin for 10 min and stained with 1% Congo red for 5 min, followed by destaining with 0.01% potassium hydroxide in 50% ethanol. Coverslips are then passed through graded ethanol concentrations for dehydration and mounted in a mounting medium and examined by fluorescent microscopy under rhodamine filter[1].

MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz.
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4354 mL 7.1771 mL 14.3542 mL 35.8855 mL
5 mM 0.2871 mL 1.4354 mL 2.8708 mL 7.1771 mL
10 mM 0.1435 mL 0.7177 mL 1.4354 mL 3.5886 mL
15 mM 0.0957 mL 0.4785 mL 0.9569 mL 2.3924 mL
20 mM 0.0718 mL 0.3589 mL 0.7177 mL 1.7943 mL
25 mM 0.0574 mL 0.2871 mL 0.5742 mL 1.4354 mL
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Congo Red Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Congo Red573-58-0Fluorescent DyeInhibitorinhibitorinhibit

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