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  4. Congo Red

Congo Red is an azo dye. Congo Red (CR) binding been used as a diagnostic test for the presence of amyloid in tissue sections.

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Congo Red

Congo Red 화학구조

CAS No. : 573-58-0

사이즈 가격 재고 수량
무료 샘플 (0.1 - 0.2 mg)   지금 신청하기  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
500 mg 해외재고보유
1 g 해외재고보유
5 g   견적 받기  
10 g   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

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고객리뷰

Based on 4 publication(s) in Google Scholar

Other Forms of Congo Red:

Congo Red Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

Congo Red is an azo dye. Congo Red (CR) binding been used as a diagnostic test for the presence of amyloid in tissue sections.

Cellular Effect
Cell Line Type Value Description References
PC-12 ED50
37.5 6
Compound: 10
Protection against beta-amyloid (25 to 35) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
Protection against beta-amyloid (25 to 35) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
[PMID: 12350137]
PC-12 ED50
39.2 6
Compound: 10
Protection against beta-amyloid (1 to 42) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
Protection against beta-amyloid (1 to 42) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
[PMID: 12350137]
In Vitro

Congo Red histochemical stain may serve as a simple screening tool for investigating if the aggregates in mutant cells have misfolded β-pleated sheet secondary structures. Congo Red histochemical dye has the ability to bind specifically to crossed β-pleated sheet structures. Wild-type HSPB1 should maintain protein homeostasis by binding proteins in non-native conformations, thereby preventing substrate aggregation. The T139M mutant, however, fails in this function and results in an accumulation of misfolded proteins, which are targeted by Congo Red for intercalating between the β-pleated sheet structures. Congo Red histochemical stain may serve as a simple tool to investigate if the aggregates in mutant cells have misfolded β-pleated sheet secondary structures[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Congo Red Related Antibodies
Clinical Trial
분자량

696.66

화학식

C32H22N6Na2O6S2
CAS No.
Appearance

Solid

Color

Pink to red

SMILES

O=S(C1=C2C=CC=CC2=C(N)C(N=NC3=CC=C(C4=CC=C(N=NC5=CC(S(=O)(O[Na])=O)=C6C=CC=CC6=C5N)C=C4)C=C3)=C1)(O[Na])=O
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

용액&용해도
In Vitro: 

DMSO : 20 mg/mL (28.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4354 mL 7.1771 mL 14.3542 mL
5 mM 0.2871 mL 1.4354 mL 2.8708 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
순도&문서
References
Cell Assay 
[1]

HeLa cells are selected for these studies due to their large cytoplasmic volume. Cells transfected with mutant or wild-type HSPB1 constructs are grown on coverslips for 24 hr and then are stained with Congo red to determine if the aggregates display amyloidogenic properties. Briefly, cells are first fixed with 10% formalin for 10 min and stained with 1% Congo red for 5 min, followed by destaining with 0.01% potassium hydroxide in 50% ethanol. Coverslips are then passed through graded ethanol concentrations for dehydration and mounted in a mounting medium and examined by fluorescent microscopy under rhodamine filter[1].

MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4354 mL 7.1771 mL 14.3542 mL 35.8855 mL
5 mM 0.2871 mL 1.4354 mL 2.8708 mL 7.1771 mL
10 mM 0.1435 mL 0.7177 mL 1.4354 mL 3.5886 mL
15 mM 0.0957 mL 0.4785 mL 0.9569 mL 2.3924 mL
20 mM 0.0718 mL 0.3589 mL 0.7177 mL 1.7943 mL
25 mM 0.0574 mL 0.2871 mL 0.5742 mL 1.4354 mL
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Congo Red Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Congo Red573-58-0Fluorescent DyeInhibitorinhibitorinhibit

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상품명:
Congo Red
Cat. No.:
HY-D0236
수량:
MCE Japan Authorized Agent:

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