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  4. DAF-FM DA

DAF-FM DA  (Synonyms: Diaminofluorescein-FM diacetate)

Cat. No.: HY-D0717 Pureza: 98.32%
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DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm).

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No. CAS : 254109-22-3

Tamaño Precio Stock Cantidad
100 μg En stock
500 μg En stock
1 mg En stock
5 mg En stock
10 mg   Obtener un presupuesto  
50 mg   Obtener un presupuesto  

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Revisión del cliente

Based on 14 publication(s) in Google Scholar

Other Forms of DAF-FM DA:

DAF-FM DA Related Antibodies

Top Publications Citing Use of Products

    DAF-FM DA purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Dec 24:170:116055.  [Abstract]

    The NO levels in the gingival tissues of mice were detected by IF assay through the DAF-FM DA staining at 4, 24 and 48 h after exposure to 2-min PBNI or 20 mM L-Arg. The pink rectangles in low-power-field images highlight the picture ROI and orange dotted lines indicate the boundary of epithelium and lamina propria.

    DAF-FM DA purchased from MedChemExpress. Usage Cited in: Redox Biol. 2023 Dec:68:102952.  [Abstract]

    The levels of intercellular nitric oxide (NO) in TAM-treated cells were analyzed using DAF-FM DA (5 μM, 30 min) with nuclear staining.

    DAF-FM DA purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Mar 17;18:91-103.  [Abstract]

    The fluorescent images of HUVECs stained with NO fluorescent probe (DAF-FM DA, 5 μMat 37 °C for 1 h) and Hoechst 33342 after being treated with UCATS under dark conditions or 808 nm NIR irradiation (1 W cm−2).

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    Descripciòn

    DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm)[1].

    In Vitro

    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1.1 Preparation of stock solution[2]
    Use anhydrous DMSO to prepare a 5 mM DAF-FM DA stock solution.
    Note: It is recommended that the DAF-FM DA stock solution be stored at -20°C or -80°C in the dark after aliquoting.
    1.2 Preparation of working solution
    Dilute the stock solution with Hank’s buffer or serum-free medium to prepare a 5 μM DAF-FM DA working solution.
    Note: Please adjust the concentration of DAF-FM DA working solution according to actual conditions and prepare it before use.
    2 Staining steps
    1) Seed breast cancer cells in a 6-well plate.
    2) Aspirate the culture medium.
    3) Add 5 μM DAF-FM DA working solution to each well and incubate at 37°C in the dark for 30 min to allow the probe to enter the cells.
    4) Replace with fresh Hank’s buffer and continue incubation for 20 min to complete the deesterification reaction.
    5) Counterstain the cell nucleus with Hoechst dye.
    6) Quantify the fluorescence intensity using a microplate reader at 488 nm excitation and 515 nm emission, and normalize to the number of cells stained with Hoechst.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DAF-FM DA Related Antibodies
    In Vivo

    Using sections of zebrafish embryos live-stained with DAF-FM DA (5 μM), could confirm that the fluorescent signals were predominantly located in areas of ongoing bone formation[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Peso molecular

    496.42

    Fòrmula

    C25H18F2N2O7
    No. CAS
    Appearance

    Solid

    Color

    Off-white to yellow

    Emission (Em)

    515
    Excitation (Ex)

    495
    SMILES

    O=C1OC2(C3=C(OC4=C2C=C(F)C(OC(C)=O)=C4)C=C(OC(C)=O)C(F)=C3)C5=C1C(N)=C(NC)C=C5
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvente y solubilidad
    In Vitro: 

    DMSO : 2.5 mg/mL (5.04 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0144 mL 10.0721 mL 20.1442 mL
    5 mM 0.4029 mL 2.0144 mL 4.0288 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Pureza y Documentación
    Dyeing Example
    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.0144 mL 10.0721 mL 20.1442 mL 50.3606 mL
    5 mM 0.4029 mL 2.0144 mL 4.0288 mL 10.0721 mL
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    DAF-FM DA Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DAF-FM DA254109-22-3Diaminofluorescein-FM diacetateFluorescent DyeNOInhibitorinhibitorinhibit

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    Nombre del producto:
    DAF-FM DA
    Cat. No.:
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