DASA-58
Based on 18 publication(s) in Google Scholar
DASA-58 is a potential pyruvate kinase isozyme (PKM2) allosteric activator. DASA-58 can be used for the research of metabolism and kinds of cancer.
For research use only. We do not sell to patients.
- Purity: 99.57%
- CAS No.: 1203494-49-8
- Formula: C19H23N3O6S2
- Molecular Weight:453.53
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Metabolism/Protease Compound Library
HY-L012
-
Anti-Cancer Compound Library
HY-L025
-
Glycolysis Compound Library
HY-L058
-
Anti-Breast Cancer Compound Library
HY-L074
-
Anti-Cancer Metabolism Compound Library
HY-L083
-
Glucose Metabolism Compound Library
HY-L092
-
Metabolic Enzyme Compound Library
HY-L146
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
Lactic Acid Metabolic Compound Library
HY-L204
-
High-Throughput Bioactive Compound Library
HY-L205
-
Lactylation Compound Library
HY-L249
Publications Citing Use of MedChemExpress (MCE) DASA-58
More- Nature. 2021 Sep;597(7875):263-267. [Abstract]
- Nat Immunol. 2018 Mar;19(3):267-278. [Abstract]
- Nat Aging. 2026 Apr;6(4):831-848. [Abstract]
- Cancer Res. 2022 Sep 16;82(18):3375-3393. [Abstract]
- Nat Commun. 2020 Jun 22;11(1):3162. [Abstract]
- Nat Commun. 2020 Feb 18;11(1):941. [Abstract]
- Adv Sci (Weinh). 2025 Dec 19:e18128. [Abstract]
- Redox Biol. 2025 Sep:85:103762. [Abstract]
- Mucosal Immunol. 2019 Nov;12(6):1280-1290. [Abstract]
- Sci Signal. 2020 Oct 27;13(655):eaay9217. [Abstract]
- J Agric Food Chem. 2022 Dec 14;70(49):15449-15463. [Abstract]
- Ecotoxicol Environ Saf. 2025 Nov 15:307:119451. [Abstract]
- Drug Des Devel Ther. 2020 May 27;14:2091-2100. [Abstract]
- Am J Pathol. 2020 Nov;190(11):2267-2281. [Abstract]
- Arch Biochem Biophys. 2019 Jul 30:670:94-103. [Abstract]
- Animals (Basel). 2021 Feb 6;11(2):425. [Abstract]
- Oncol Lett. 2024 Jul 19;28(3):443. [Abstract]
- bioRxiv. 2026 Jan 20.
Customer Validation & Images
-
Bio/Physico-chemical Assay
-
Cell Proliferation/Viability Assay
-
Cell Imaging/Staining
-
WB
-
Cell Proliferation/Viability Assay
Biological Activity
DASA-58 (15 μM; 2 h) potentiates the antitumor effects of other metabolic stressors[1].
DASA-58 (15 μM; 24 h, 72 h) enhances pyruvate kinase activity in breast cancer cells without a clear effect on proliferation[1].
DASA-58 (30 μM, 60 μM; 0-72 h) enhances extracellular acidification and lactate levels in BCa cell lines and induce extracellular acidification levels in prostate cancer cell lines[1].
DASA-58 (15 μM, 30 μM; 0-72 h) affects respiration levels in BCa cells without an indication of mitochondrial damage[1].
DASA-58 (15 μM; 0-72 h) not rescues TXNIP levels in any combintion and mitochondrial inhibitors enhance PKM2 effects on activating AMPK signaling (T172 phosphorylation of AMPK) [1].
DASA-58 (15 μM; 0-72 h) leads to depletion in TXNIP levels independent of AMPK and ER signaling, and not through enhanced proteasomal degradation but rather depleted upstream glycolytic intermediates[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Five breast cancer cell lines (BCa cells)
(MDA MB 231, MDA MB 468, HCC 1443, T47-D, MCF7, LnCap, PC3, and DU145) -
Concentration:15 μM
-
Incubation Time:2 h
-
Result:Could be exploited by other metabolic stressors.
-
Cell Line:BCa cells
-
Concentration:15 μM
-
Incubation Time:24 h, 72 h
-
Result:Showed comparable PKM2 protein levels in five breast cancer cell lines, except HCC1443 cells and MDA MB 468 that showed the highest and lowest PKM2 protein levels, respectively.
Not changed PKM2 levels in five breast cancer cell lines but seemingly reduced TXNIP levels in cells expressing detectable TXNIP levels.
Chemical Information
-
CAS No. 1203494-49-8
-
Appearance Solid
-
Molecular Weight 453.53
-
Formula C19H23N3O6S2
-
Color White to off-white
-
SMILES
NC1=CC=CC(S(=O)(N2CCN(S(=O)(C3=CC=C(OCCO4)C4=C3)=O)CCC2)=O)=C1
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (18)
-
Journal Impact Factor
-
Most Recent
-
Nature
2021 Sep;597(7875):263-267. PMID: 34408323 -
Nat Immunol
2018 Mar;19(3):267-278. PMID: 29358707
DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2018 Mar;19(3):267-278. [Abstract]
CellTrace Violet profile from in vitro stimulated splenic B cells stimulated for 62 hours as indicated above the histogram overlays in the presence of DASA-58 (12.5 μM) or vehicle control (as the highest concentration of DASA-58).
DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2018 Mar;19(3):267-278. [Abstract]
DASA-58 (12.5 μM) reduced the proliferation of mouse B cells in response to in vitro stimulation with anti-CD40+IL-4+IL-5 or anti-IgM+IL-4.
-
Nat Aging
The glycolytic metabolite phosphoenolpyruvate restricts cGAS-driven inflammation to promote healthy aging. [Abstract]2026 Apr;6(4):831-848. PMID: 41792330 -
Cancer Res
Pharmacologic Targeting of TFIIH Suppresses KRAS-Mutant Pancreatic Ductal Adenocarcinoma and Synergizes with TRAIL. [Abstract]2022 Sep 16;82(18):3375-3393. PMID: 35819261 -
Nat Commun
Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. [Abstract]2020 Jun 22;11(1):3162. PMID: 32572027
DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Jun 22;11(1):3162. [Abstract]
The glycolysis analysis of HepG2 cells overexpressing HULC treated by FGFR1 inhibitor PD166866 (2.5 μM), PKM2 activator DASA-58 (30 μM, 0-100 min), or LDHA inhibitor GSK2837808A (10 μM). DMSO was added instead in the control groups.
DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Jun 22;11(1):3162. [Abstract]
Proliferation of HepG2 cells with HULC knockdown (left panel) or overexpression (right panel) was measured with a CCK8 assay. For rescue experiments, HepG2-HULC cells were treated by FGFR1 inhibitor PD 166866 (2.5 μM), PKM2 activator DASA-58 (30 μM), or LDHA inhibitor GSK2837808A (10 μM), respectively.
-
Nat Commun
Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection. [Abstract]2020 Feb 18;11(1):941. PMID: 32071304
DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Feb 18;11(1):941. [Abstract]
Primary astrocytes were pretreated with 50 µM DASA-58 for 1 h and then stimulated with 10 µM quinpirole for 12 h. PKM2 and Nrf2 proximity ligation signals.
DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Feb 18;11(1):941. [Abstract]
Primary astrocytes were pretreated with 50 µM DASA-58 for 1 h and then stimulated with 10 µM quinpirole for 12 h. DASA-58 treatment attenuated DRD2 activation-induced PKM2 dimerization.
-
Adv Sci (Weinh)
F13A1-Mediated Macrophage Activation Promotes MASH Progression via the PKM2/HIF1A Pathway. [Abstract]2025 Dec 19:e18128. PMID: 41417477 -
Redox Biol
Activation of sclerostin inhibits Isg20-Mediated aerobic glycolysis ameliorating renal Fibrosis: the renoprotective mechanism of hederagenin in CKD. [Abstract]2025 Sep:85:103762. PMID: 40644925 -
Mucosal Immunol
Intestinal epithelial PKM2 serves as a safeguard against experimental colitis via activating β-catenin signaling. [Abstract]2019 Nov;12(6):1280-1290. PMID: 31462699 -
Sci Signal
2020 Oct 27;13(655):eaay9217. PMID: 33109748 -
J Agric Food Chem
Tetrahydroxy Stilbene Glucoside Alleviates Ischemic Stroke by Regulating Conformation-Dependent Intracellular Distribution of PKM2 for M2 Macrophage Polarization. [Abstract]2022 Dec 14;70(49):15449-15463. PMID: 36468551 -
Ecotoxicol Environ Saf
MC-LR triggers trophoblast ferroptosis at the maternal-fetal interface by directly targeting PKM2 to reprogram metabolism. [Abstract]2025 Nov 15:307:119451. PMID: 41273830 -
Drug Des Devel Ther
Dihydroartemisinin Inhibits the Proliferation of Leukemia Cells K562 by Suppressing PKM2 and GLUT1 Mediated Aerobic Glycolysis. [Abstract]2020 May 27;14:2091-2100. PMID: 32546972
DASA-58 purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2020 May 27;14:2091-2100. [Abstract]
The growth inhibition of DHA after 24 hrs together with 40 μM DASA-58 (a PKM2 agonist). Tumor cell growth inhibition is determined by CCK-8 after treated with various concentrations of DHA at different times.
-
Am J Pathol
Pyruvate Kinase M2 Tetramerization Protects against Hepatic Stellate Cell Activation and Liver Fibrosis. [Abstract]2020 Nov;190(11):2267-2281. PMID: 32805235 -
Arch Biochem Biophys
Chaetocin attenuates gout in mice through inhibiting HIF-1α and NLRP3 inflammasome-dependent IL-1β secretion in macrophages. [Abstract]2019 Jul 30:670:94-103. PMID: 31255694 -
Animals (Basel)
Glycolysis Combined with Core Pluripotency Factors to Promote the Formation of Chicken Induced Pluripotent Stem Cells. [Abstract]2021 Feb 6;11(2):425. PMID: 33562170 -
Oncol Lett
Interference with ENO2 promotes ferroptosis and inhibits glycolysis in clear cell renal cell carcinoma by regulating Hippo‑YAP1 signaling. [Abstract]2024 Jul 19;28(3):443. PMID: 39091581 -
Solvent & Solubility
DMSO : 125 mg/mL (275.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Portuguese - PT (392 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2049 mL | 11.0246 mL | 22.0493 mL | 55.1231 mL |
| 5 mM | 0.4410 mL | 2.2049 mL | 4.4099 mL | 11.0246 mL | |
| 10 mM | 0.2205 mL | 1.1025 mL | 2.2049 mL | 5.5123 mL | |
| 15 mM | 0.1470 mL | 0.7350 mL | 1.4700 mL | 3.6749 mL | |
| 20 mM | 0.1102 mL | 0.5512 mL | 1.1025 mL | 2.7562 mL | |
| 25 mM | 0.0882 mL | 0.4410 mL | 0.8820 mL | 2.2049 mL | |
| 30 mM | 0.0735 mL | 0.3675 mL | 0.7350 mL | 1.8374 mL | |
| 40 mM | 0.0551 mL | 0.2756 mL | 0.5512 mL | 1.3781 mL | |
| 50 mM | 0.0441 mL | 0.2205 mL | 0.4410 mL | 1.1025 mL | |
| 60 mM | 0.0367 mL | 0.1837 mL | 0.3675 mL | 0.9187 mL | |
| 80 mM | 0.0276 mL | 0.1378 mL | 0.2756 mL | 0.6890 mL | |
| 100 mM | 0.0220 mL | 0.1102 mL | 0.2205 mL | 0.5512 mL |
Powered by Bioz