1. Metabolic Enzyme/Protease
  2. Pyruvate Kinase
  3. DASA-58

DASA-58 

Cat. No.: HY-19330 Purity: 99.67%
COA Handling Instructions

DASA-58 is a potential pyruvate kinase isozyme (PKM2) allosteric activator. DASA-58 can be used for the research of metabolism and kinds of cancer.

For research use only. We do not sell to patients.

DASA-58 Chemical Structure

DASA-58 Chemical Structure

CAS No. : 1203494-49-8

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 88 In-stock
Solution
10 mM * 1 mL in DMSO USD 88 In-stock
Solid
1 mg USD 35 In-stock
5 mg USD 80 In-stock
10 mg USD 130 In-stock
50 mg USD 420 In-stock
100 mg USD 670 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 13 publication(s) in Google Scholar

Top Publications Citing Use of Products

    DASA-58 purchased from MedChemExpress. Usage Cited in: Drug Des Dev Ther. 2020 May 27;14:2091-2100.  [Abstract]

    The growth inhibition of DHA after 24 hrs together with 40 μM DASA-58 (a PKM2 agonist). Tumor cell growth inhibition is determined by CCK-8 after treated with various concentrations of DHA at different times.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DASA-58 is a potential pyruvate kinase isozyme (PKM2) allosteric activator. DASA-58 can be used for the research of metabolism and kinds of cancer[1].

    In Vitro

    DASA-58 (15 μM; 2 h) potentiates the antitumor effects of other metabolic stressors[1].
    ? DASA-58 (15 μM; 24 h, 72 h) enhances pyruvate kinase activity in breast cancer cells without a clear effect on proliferation[1].
    ? DASA-58 (30 μM, 60 μM; 0-72 h) enhances extracellular acidification and lactate levels in BCa cell lines and induce extracellular acidification levels in prostate cancer cell lines[1].
    ? DASA-58 (15 μM, 30 μM; 0-72 h) affects respiration levels in BCa cells without an indication of mitochondrial damage[1].
    ? DASA-58 (15 μM; 0-72 h) not rescues TXNIP levels in any combintion and mitochondrial inhibitors enhance PKM2 effects on activating AMPK signaling (T172 phosphorylation of AMPK) [1].
    ? DASA-58 (15 μM; 0-72 h) leads to depletion in TXNIP levels independent of? AMPK and ER signaling, and not through enhanced? proteasomal degradation but rather depleted upstream glycolytic intermediates[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: Five breast cancer cell lines (BCa cells)
    (MDA MB 231, MDA MB 468, HCC 1443, T47-D, MCF7, LnCap, PC3, and DU145)
    Concentration: 15 μM
    Incubation Time: 2 h
    Result: Could be exploited by other metabolic stressors.

    Western Blot Analysis[1]

    Cell Line: BCa cells
    Concentration: 15 μM
    Incubation Time: 24 h, 72 h
    Result: Showed comparable PKM2 protein levels in five breast cancer cell lines, except HCC1443 cells and MDA MB 468 that showed the highest and lowest PKM2 protein levels, respectively.
    Not changed PKM2 levels in five breast cancer cell lines but seemingly reduced TXNIP levels in cells expressing detectable TXNIP levels.
    Molecular Weight

    453.53

    Formula

    C19H23N3O6S2

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC1=CC=CC(S(=O)(N2CCN(S(=O)(C3=CC=C(OCCO4)C4=C3)=O)CCC2)=O)=C1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 35 mg/mL (77.17 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2049 mL 11.0246 mL 22.0493 mL
    5 mM 0.4410 mL 2.2049 mL 4.4099 mL
    10 mM 0.2205 mL 1.1025 mL 2.2049 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.59 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.59 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (4.59 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.67%

    References
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    DASA-58 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    × = ×
    C1   V1   C2   V2

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    Product Name:
    DASA-58
    Cat. No.:
    HY-19330
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