DBeQ (Synonyms: JRF 12)

Name: DBeQ
Brand: MedChemExpress
SKU: HY-15945
Rating: 5.0 (7 reviews)

Cat. No.: HY-15945 Purity: 98.79%: Data Sheet
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DBeQ is a selective, potent, reversible, and ATP-competitive p97 inhibitor, with an IC₅₀ value of 1.5 μM and 1.6 μM for p97(wt) and p97(C522A), respectively; DBeQ also inhibits Vps4 with an IC₅₀ of 11.5 μM.

For research use only. We do not sell to patients.

CAS No. : 177355-84-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 7 publication(s) in Google Scholar

7 Publications Citing Use of MCE DBeQ

•Vet Microbiol. 2022 Sep:272:109511. [Abstract]

RT-PCR

Bio/Physico-chemical Assay

Cell Imaging/Staining

: DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]; We examined the turnover of PLIN2 controlled by nsp6 and observed that nsp6 increased the degradation of PLIN2 protein, while the treatment of MG132 or the ERAD inhibitor DBeQ blocked nsp6-mediated reduction in PLIN2 protein level; CQ was used as a negative control.

: DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]; Confocal microscopy of Huh-7 cells transfected with mCherry-nsp6 for 24 h and then treated with 5 μM DBeQ for an additional 12 h.

: DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]; HeLa-mCEACAM1 cells were infected with MHV-A59 and treated with DBeQ (0-5 μM). The cell lysates were subjected to qRT-PCR.

: DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]; HeLa-mCEACAM1 cells were infected with MHV-A59 and treated with DBeQ (0-5 μM, 12-24 h). The cell lysates were subjected to immunoblot analysis.

: DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]; HeLa-mCEACAM1 cells were infected with MHV-A59 and treated with DBeQ (0-5 μM). The supernatant was used for plaque assay.

: DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]; 17Cl-1 cells were pre-treated with 5 μM DBeQ overnight and then infected with MHV-A59 at MOI of 5 for 12 h.

: DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]; 17Cl-1 cells were infected with MHV at MOI of 5 for 2 h and treated with 5 μM DBeQ for 10 h.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 1.5 μM (p97)^[1], 11.5 μM (Vps4)^[2]

Cellular Effect

Cell Line	Type	Value	Description	References
HT-29	IC₅₀	4.72 μM Compound: 3e	Cytotoxicity against human HT-29 cells after 72 hrs by neutral red dye assay Cytotoxicity against human HT-29 cells after 72 hrs by neutral red dye assay	[PMID: 21700369]
HT-29	IC₅₀	4.72 μM Compound: I	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis	[PMID: 35694693]
J774.A1	EC₅₀	0.67 μM Compound: 10	Antileishmanial activity against intracellular amastigote stage of Leishmania donovani MHOM/ET/67/L82 infected in mouse J774A1 cells by colorimetric assay Antileishmanial activity against intracellular amastigote stage of Leishmania donovani MHOM/ET/67/L82 infected in mouse J774A1 cells by colorimetric assay	[PMID: 24874647]
J774.A1	EC₅₀	1.4 μM Compound: 10	Antileishmanial activity against intracellular amastigote stage of Leishmania amazonensis LV78 infected in mouse J774A1 cells by colorimetric assay Antileishmanial activity against intracellular amastigote stage of Leishmania amazonensis LV78 infected in mouse J774A1 cells by colorimetric assay	[PMID: 24874647]
J774.A1	EC₅₀	5.5 μM Compound: 1	Cytotoxicity against mouse J774A.1 cells assessed as inhibition of cell viability after 72 hrs by MTT assay Cytotoxicity against mouse J774A.1 cells assessed as inhibition of cell viability after 72 hrs by MTT assay	[PMID: 25749014]
J774.A1	EC₅₀	5.5 μM Compound: 10	Cytotoxicity against mouse J774A1 cells after 72 hrs by MTT assay Cytotoxicity against mouse J774A1 cells after 72 hrs by MTT assay	[PMID: 24874647]
MDA-MB-231	IC₅₀	1.79 μM Compound: 3e	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by neutral red dye assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by neutral red dye assay	[PMID: 21700369]
MDA-MB-231	IC₅₀	1.79 μM Compound: I	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis	[PMID: 35694693]
T-24	IC₅₀	5.96 μM Compound: 3e	Cytotoxicity against human T24 cells after 72 hrs by neutral red dye assay Cytotoxicity against human T24 cells after 72 hrs by neutral red dye assay	[PMID: 21700369]
T-24	IC₅₀	5.96 μM Compound: I	Antiproliferative activity against human T24 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis Antiproliferative activity against human T24 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis	[PMID: 35694693]

In Vitro

DBeQ is a ATP-competitive p97 inhibitor, with an IC₅₀ value of 1.5 μM and 1.6 μM for p97(wt) and p97(C522A), respectively. DBeQ inhibits p97 competitively with respect to ATP, with a K_i of 3.2 ± 0.4 μM. DBeQ inhibits degradation of the p97-dependent substrate UbG76V-GFP, with IC₅₀ value of 2.6 μM. DBeQ (10 μM) also significantly suppresses degradation of TCRα-GFP, induces CHOP but does not increase p21 level. Moreover, DBeQ inhibits the viability of MRC-5, Hek293, HeLa and RPMI8226 cells, with GI₅₀s of 6.6 ± 2.9, 4 ± 0.6, 3.1 ± 0.5 and 1.2 ± 0.3, respectively^[1]. DBeQ potently inhibits the AAA ATPase p97 by specifically binding to the ATPase site of its D2 domain (p97D2). DBeQ also inhibits Vps4, with an IC₅₀ of 11.5 μM. Furthermore, DBeQ (30 μM) inhibits hyphal growth of the wild-type cell (strain YLZ0)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

340.42

Formula

C₂₂H₂₀N₄

CAS No.

177355-84-9

Appearance

Solid

Color

White to off-white

SMILES

C1(NCC2=CC=CC=C2)=NC(NCC3=CC=CC=C3)=C4C=CC=CC4=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 35.71 mg/mL (104.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9375 mL	14.6877 mL	29.3755 mL
5 mM	0.5875 mL	2.9375 mL	5.8751 mL
10 mM	0.2938 mL	1.4688 mL	2.9375 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.79%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (252 KB) HNMR (280 KB) RP-HPLC (251 KB) LCMS (340 KB)

Handling Instructions (2659 KB)

References

[1]. Chou TF, et al.Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways. Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):4834-9. [Content Brief]

[2]. Zhang Y, et al. The AAA ATPase Vps4 Plays Important Roles in Candida albicans Hyphal Formation and is Inhibited by DBeQ. Mycopathologia. 2016 Jun;181(5-6):329-39. [Content Brief]

Cell Assay
^[1]

Cells are seeded on a 384-well solid white plate (5,000 cells/well). Cells are transfected with luciferase siRNA or p97 siRNA (10 nM) for 48 h or treated with DBeQ for the indicated amount of time. Caspase-3/7 Glo, caspase-6 Glo, caspase-8 Glo, or caspase-9 Glo is added into each well and mixed by shaking at 500 rpm for 1 min. Luminescence signal is determined after incubation at room temperature for 1 h. Cellular viability is determined with CellTiter-Glo reagent. To determine the IC₅₀ of cellular viability, cells are treated with MG132 or DBeQ at seven concentrations (threefold serial dilutions starting at 33 μM) for 48 h. IC₅₀ values are calculated from fitting the percentage of luminescence signal normalized to DMSO treated cells)^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Chou TF, et al.Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways. Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):4834-9. [Content Brief]

[2]. Zhang Y, et al. The AAA ATPase Vps4 Plays Important Roles in Candida albicans Hyphal Formation and is Inhibited by DBeQ. Mycopathologia. 2016 Jun;181(5-6):329-39. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9375 mL	14.6877 mL	29.3755 mL	73.4387 mL
	5 mM	0.5875 mL	2.9375 mL	5.8751 mL	14.6877 mL
	10 mM	0.2938 mL	1.4688 mL	2.9375 mL	7.3439 mL
	15 mM	0.1958 mL	0.9792 mL	1.9584 mL	4.8959 mL
	20 mM	0.1469 mL	0.7344 mL	1.4688 mL	3.6719 mL
	25 mM	0.1175 mL	0.5875 mL	1.1750 mL	2.9375 mL
	30 mM	0.0979 mL	0.4896 mL	0.9792 mL	2.4480 mL
	40 mM	0.0734 mL	0.3672 mL	0.7344 mL	1.8360 mL
	50 mM	0.0588 mL	0.2938 mL	0.5875 mL	1.4688 mL
	60 mM	0.0490 mL	0.2448 mL	0.4896 mL	1.2240 mL
	80 mM	0.0367 mL	0.1836 mL	0.3672 mL	0.9180 mL
	100 mM	0.0294 mL	0.1469 mL	0.2938 mL	0.7344 mL

DBeQ Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DBeQ177355-84-9JRF 12JRF12JRF-12p97AutophagyApoptosisVCPCdc48Inhibitorinhibitorinhibit

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DBeQ (Synonyms: JRF 12)

Customer Review

DBeQ Related Antibodies

7 Publications Citing Use of MCE DBeQ

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•Related Screening Libraries:

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Biological Activity

Protocol

Purity & Documentation

References

Customer Review

DBeQ Related Antibodies

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Complete Stock Solution Preparation Table

DBeQ Related Classifications

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