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  4. Di-8-ANEPPS

Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .

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Di-8-ANEPPS

Di-8-ANEPPS 構造式

CAS 番号 : 157134-53-7

容量 価格（税別） 在庫状況 数量
1 mg $88 在庫あり
5 mg $310 在庫あり
10 mg $465 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 1 publication(s) in Google Scholar

Di-8-ANEPPS 関連抗体

Top Publications Citing Use of Products

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  • 純度とドキュメンテーション

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製品説明

Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .

体外実験

A mouse cell staining with Di-8-ANEPPS is applied voltage clamp pulses and immersed in Na+-containing solution. The Di-8-ANEPPS signal, which largely reflects t system voltage, has an asymmetrical positive component upon application of depolarizing pulses. This is interpreted as reflecting a propagating action potential, escaping from the voltage-clamped plasma membrane. Its temporal properties are not unlike those of the "center" signal. Notably, the peak of the signal occurrs 0.3 ms after the leading edge of the depolarizing pulse[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Di-8-ANEPPS 関連抗体
分子量

592.87

分子式

C36H52N2O3S
Emission (Em)

635
Excitation (Ex)

465
CAS 番号
Appearance

Solid

Color

Brown to red

SMILES

O=S(CCC[N+]1=CC=C(/C=C/C2=CC=C3C=C(N(CCCCCCCC)CCCCCCCC)C=CC3=C2)C=C1)([O-])=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

-20°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.
溶剤 & 溶解度
体外: 

DMSO : 1 mg/mL (1.69 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6867 mL 8.4336 mL 16.8671 mL
5 mM --- --- ---
10 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

Animal weight
(per animal)

g

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
純度とドキュメンテーション

純度: 99.84%

COA (248 KB) HNMR (320 KB) RP-HPLC (254 KB) MS (75 KB)

取扱説明書 (2659 KB)
参考文献
細胞実験
[1]

To implement the technique, cells enzymatically isolated from mouse flexor digitorum brevis (FDB) muscle are stained by brief exposure to a saline with Di-8-ANEPPS and then washed in solution[1].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6867 mL 8.4336 mL 16.8671 mL 42.1678 mL
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Di-8-ANEPPS Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Di-8-ANEPPS157134-53-7Fluorescent DyeInhibitorinhibitorinhibit

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製品名:
Di-8-ANEPPS
製品番号:
HY-101891
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