2. Cytoskeleton
  3. Gap Junction Protein
  4. Gap 27

Gap 27, a synthetic connexin43 mimetic peptide, is a gap junction inhibitor. Gap 27 possesses conserved sequence homology to a portion of the second extracellular loop leading into the fourth transmembrane connexin segment.

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CAS 番号 : 198284-64-9

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1 mg $60 在庫あり
5 mg $130 在庫あり
10 mg $200 在庫あり
25 mg $400 在庫あり
50 mg $600 在庫あり
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カスタマーレビュー

Based on 7 publication(s) in Google Scholar

Gap 27 関連抗体

Top Publications Citing Use of Products

    Gap 27 purchased from MedChemExpress. Usage Cited in: Brain Behav Immun. 2026 Jan:131:106176.  [Abstract]

    Gap 27 (1 mg/mL; 1 μL bilaterally microinjected into the medial prefrontal cortex (mPFC) over 10 min) significantly decreased Cx43 expression levels in the mPFC of mice, whereas RBD administration did not further alter Cx43 expression.

    Gap 27 purchased from MedChemExpress. Usage Cited in: Brain Behav Immun. 2026 Jan:131:106176.  [Abstract]

    Gap 27 (1 mg/mL; 1 μL bilaterally microinjected into the medial prefrontal cortex (mPFC) over 10 min) pretreatment markedly decreased sucrose preference in the sucrose preference test (SPT), increased immobility time in the forced swim test (FST), reduced locomotor activity in the open field test (OFT), and prolonged immobility time in the tail suspension test (TST) compared to control mice.

    Gap 27 purchased from MedChemExpress. Usage Cited in: Brain Behav Immun. 2026 Jan:131:106176.  [Abstract]

    Gap 27 (1 mg/mL; 1 μL bilaterally microinjected into the medial prefrontal cortex (mPFC) over 10 min) significantly reduced the proportion of Cx43 fluorescent spots at the interstitial junctions of astrocytes in the medial prefrontal cortex of mice, while RBD treatment had no such effect.

    Gap 27 purchased from MedChemExpress. Usage Cited in: Brain Behav Immun. 2026 Jan:131:106176.  [Abstract]

    Representative immunoblots of the cGAS-STING signaling pathway in primary astrocytes in the Vehicle, Gap 27 (200 μM; 24 h), and Gap 27 + RBD (10 nM; 24 h) groups were shown. The results showed that Gap 27 treatment significantly increased protein levels of cGAS and activated components of the cGAS-STING pathway, including p-STING/STING, p-TBK1/TBK1, and p-IRF3/IRF3. Notably, these protein levels remained unchanged when RBD was added to Gap 27-treated astrocytes.

    Gap 27 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702.  [Abstract]

    The PWT and PWL of mice after intrathecal injection of Gap27 (14.4, 43.2, 144 nM; 10 μL) for 2 h, compared with the VCR group at the same point in time, n = 8 mice/group. The results indicated that, compared with the VCR group at the same time point, intrathecal injections of Gap27 dose-dependently reversed mechanical allodynia and heat hyperalgesia at 2 h after treatment.

    Gap 27 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702.  [Abstract]

    Western blotting and quantitative analysis of Cx43 protein expression in spinal cord of mice treated with Gap 27 (14.4, 43.2, 144 nM; 10 μL; intrathecal injection), n = 3 mice/group. The results suggested that the protein levels of Cx43 declined remarkably after the first intrathecal injection of Gap 27 for 36 h.

    Gap 27 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702.  [Abstract]

    qRT-PCR analysis of spinal cord Cx43 mRNA level after Gap 27 (14.4, 43.2, 144 nM; 10 μL; intrathecal injection) injection, n = 4 mice/group. The results suggested that the mRNA levels of Cx43 declined remarkably after the first intrathecal injection of Gap27 for 36 h.

    Gap 27 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702.  [Abstract]

    Immunofluorescence images of Cx43 intensity in the astrocyte cultures following treatment with Gap 27 (30 μM) for 24 h, n = 3 cultures/group. The results showed that VCR treatment (3 nM, 24 h) evoked a pronounced upregulation in the fluorescence intensity of astrocytes Cx43 and this was reversed by Gap 27 (30 μM).

    Gap 27 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702.  [Abstract]

    The Western blotting analysis proved that the increase in Cx43 expression induced by VCR was reduced by 41.2% in astrocyte cultures treated with Gap 27 (30 μM) for 24 h.

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    製品説明

    Gap 27, a synthetic connexin43 mimetic peptide, is a gap junction inhibitor. Gap 27 possesses conserved sequence homology to a portion of the second extracellular loop leading into the fourth transmembrane connexin segment[1][2].

    体外実験

    Gap 27 causes a remarked decrease in the number of both TRAP-positive mononuclear and multinucleated rat osteoclasts cultured on bovine bone slices. The activity of the remaining osteoclasts is found to be diminished by measuring the percentage of osteoclasts with actin rings of all TRAP-positive cells. In addition, the resorbed area in the treated cultures is greatly diminished[1].
    Incubation of the carotid artery with the gap junction inhibitor Gap 27 (500 μM) essentially abolishes the hyperpolarization to acetylcholine but it is without effect on that to levcromakalim. In the guinea-pig isolated internal carotid artery, Gap 27 inhibits acetylcholine-induced, endothelium-dependent hyperpolarizations[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Gap 27 関連抗体
    体内実験

    Gap 27 (300 μM) inhibits relaxation by 40% in thoracic aorta and the superior mesenteric artery. Gap 27 also attenuates the endothelium-dependent component of the relaxation induced by ATP in thoracic aorta. [3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    1304.53

    分子式

    C60H101N15O17
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile
    Sequence Shortening

    SRPTEKTIFII
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Sealed storage, away from moisture
    Powder -80°C 2 years
      -20°C 1 year
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    H2O : ≥ 33.33 mg/mL (25.55 mM)

    DMSO : 22.22 mg/mL (17.03 mM; ultrasonic and adjust pH to 2 with 1 M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.7666 mL 3.8328 mL 7.6656 mL
    5 mM 0.1533 mL 0.7666 mL 1.5331 mL
    10 mM 0.0767 mL 0.3833 mL 0.7666 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    純度とドキュメンテーション

    純度: 99.90%

    COA (249 KB) RP-HPLC (253 KB) MS (68 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [1]

    Bone cell cultures are cultured for 48 hours with three different treatments (control, heptanol and Gap 27). After the culture period, bone slices are fixed. The cells are stained for tartrate-resistant acid phosphatase (TRAP). To visualise the nuclei, the cells are incubated with the DNA-binding fluorochrome Hoechst 33258 (1 mg/mL stock diluted 1:800 in PBS) for 10 minutes at room temperature. The numbers of mononuclear and multinucleated TRAP-positive cells on each bone slice are counted[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 0.7666 mL 3.8328 mL 7.6656 mL 19.1640 mL
    5 mM 0.1533 mL 0.7666 mL 1.5331 mL 3.8328 mL
    10 mM 0.0767 mL 0.3833 mL 0.7666 mL 1.9164 mL
    15 mM 0.0511 mL 0.2555 mL 0.5110 mL 1.2776 mL
    H2O 20 mM 0.0383 mL 0.1916 mL 0.3833 mL 0.9582 mL
    25 mM 0.0307 mL 0.1533 mL 0.3066 mL 0.7666 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Gap 27198284-64-9Gap27Gap-27Gap Junction ProteinInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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