2. Others Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Fluorescent Dye Reactive Oxygen Species (ROS)
  4. Lucigenin

Lucigenin is a chemiluminescence probe. Lucigenin can be used to detect the production of endogenous superoxide anion radical (O2-). Lucigenin is extremely sensitive to chloride ions, while it combined with chloride ions, the fluorescence will be quenched. Lucigenin also can be used as a chloride indicator. Ex/Em=455/505 nm.

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CAS No. : 2315-97-1

Size Prix Stock Quantité
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
5 mg En stock
10 mg En stock
50 mg En stock
100 mg En stock
500 mg En stock
1 g   Obtenir un devis  
5 g   Obtenir un devis  

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Based on 7 publication(s) in Google Scholar

Lucigenin Related Antibodies

Top Publications Citing Use of Products

    Lucigenin purchased from MedChemExpress. Usage Cited in: Mar Drugs. 2025 Jul 30;23(8):309.  [Abstract]

    Micrographs of conchocelis stained with lucigenin. Scale bars: 50 μm. Conchocelis was treated with SPM along with NOX inhibitor DPI for 8 h and then stained with Lucigenin (0.1 μM) for 30 min.

    Lucigenin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Nov:77:103397.  [Abstract]

    Lucigenin (10 μM, at room temperature for 15 min). The level of ROS production of HASMCs in indicated groups (n = 5 independent experiments).

    Lucigenin purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Mar 25;21(1):218.  [Abstract]

    Lucigenin(100 μM). Superoxide production by NADPH oxidase was measured in total cell homogenates.

    Lucigenin purchased from MedChemExpress. Usage Cited in: J Fungi (Basel). 2021 Nov 11;7(11):955.  [Abstract]

    Lucigenin (10 mM). The green fluorescence signals in hyphae. The light (left), fluorescence confocal (middle), and merge (right) microscope observations of strains hypha. Bar = 10 µm.

    Lucigenin purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2017 Dec;40(6):1803-1817.  [Abstract]

    Lucigenin(10 µM). Quantitative analysis of NADPH oxidase activity in HPMECs, as determined by lucigenin assay.

    • Activité biologique

    • Pureté et documentation

    • Références

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    Description

    Lucigenin is a chemiluminescence probe. Lucigenin can be used to detect the production of endogenous superoxide anion radical (O2-). Lucigenin is extremely sensitive to chloride ions, while it combined with chloride ions, the fluorescence will be quenched. Lucigenin also can be used as a chloride indicator. Ex/Em=455/505 nm[1].

    In Vitro

    Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
    Preparation of Lucigenin working solution
    1.1 Preparation of the stock solution
    Dissolve 1 mg of Lucigenin in 0.1959 mL of DMSO to obtain 10 mM of Lucigenin.
    Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles.
    1.2 Preparation of Lucigenin working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of Lucigenin working solution.
    Note: Please adjust the concentration of Lucigenin working solution according to the actual situation.

    Cell staining
    2.1 Cell preparation.
    For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
    For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
    2.2 Add 1 mL of Lucigenin working solution, and then incubate at room temperature for 15 minutes.
    2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    2.4 Wash twice with PBS, 5 minutes each time.
    2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Lucigenin Related Antibodies
    Masse moléculaire

    510.50

    Formule

    C28H22N4O6
    CAS No.
    Appearance

    Solid

    Color

    Yellow to orange

    Emission (Em)

    505
    Excitation (Ex)

    455
    SMILES

    C[N+]1=C2C=CC=CC2=C(C3=C4C=CC=CC4=[N+](C)C5=C3C=CC=C5)C6=C1C=CC=C6.O=N([O-])=O.O=N([O-])=O
    Livraison

    Room temperature in continental US; may vary elsewhere.
    Stockage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvant et solubilité
    In Vitro: 

    DMSO : 57.14 mg/mL (111.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9589 mL 9.7943 mL 19.5886 mL
    5 mM 0.3918 mL 1.9589 mL 3.9177 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Calculateur de molarité

    • Calculateur de dilution

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Pureté et documentation

    Purity: 99.01%

    SDS (608 KB)

    COA (259 KB)

    Instruction de manipulation (2659 KB)
    Dyeing Example
    Références

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9589 mL 9.7943 mL 19.5886 mL 48.9716 mL
    5 mM 0.3918 mL 1.9589 mL 3.9177 mL 9.7943 mL
    10 mM 0.1959 mL 0.9794 mL 1.9589 mL 4.8972 mL
    15 mM 0.1306 mL 0.6530 mL 1.3059 mL 3.2648 mL
    20 mM 0.0979 mL 0.4897 mL 0.9794 mL 2.4486 mL
    25 mM 0.0784 mL 0.3918 mL 0.7835 mL 1.9589 mL
    30 mM 0.0653 mL 0.3265 mL 0.6530 mL 1.6324 mL
    40 mM 0.0490 mL 0.2449 mL 0.4897 mL 1.2243 mL
    50 mM 0.0392 mL 0.1959 mL 0.3918 mL 0.9794 mL
    60 mM 0.0326 mL 0.1632 mL 0.3265 mL 0.8162 mL
    80 mM 0.0245 mL 0.1224 mL 0.2449 mL 0.6121 mL
    100 mM 0.0196 mL 0.0979 mL 0.1959 mL 0.4897 mL
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    Lucigenin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Lucigenin2315-97-1NSC-151912 L-6868NSC151912NSC 151912L6868L 6868L-6868Fluorescent DyeReactive Oxygen Species (ROS)Inhibitorinhibitorinhibit

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