Oxazine 1 perchlorate

Name: Oxazine 1 perchlorate
Brand: MedChemExpress
SKU: HY-101901
Rating: 4.5 (1 reviews)

Cat. No.: HY-101901 Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Oxazine 1 perchlorate is a symmetric cationic dye (λ_ex=653 nm, λ_em=666 nm).

For research use only. We do not sell to patients.

Oxazine 1 perchlorate Chemical Structure

CAS No. : 24796-94-9

Size	Price	Stock	Quantity
5 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 304	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Oxazine 1 perchlorate

•Phys Chem Chem Phys. 2022 May 18;24(19):11520-11526. [Abstract]

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Oxazine 1 perchlorate is a symmetric cationic dye (λ_ex=653 nm, λ_em=666 nm).

In Vitro

Oxazine 1 perchlorate (OX1) absorbs and emits light at about 653 and 666 nm in dilute aqueous solutions, respectively. It is soluble over a very wide range of polar solvents. The optical spectrum of Oxazine 1 perchlorate typically possesses an intense absorption band (λ_max), which is neighbored by a shoulder at shorter wavelengths. Oxazine 1 perchlorate appears to exist almost in its monomeric form at concentrations below about 1×10^-4 M in aqueous solutions. As it is expected, similar to polar ordinary liquid solvents, Oxazine 1 perchlorate shows only single fluorescence band in polar anisotropic media^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

423.89

Formula

C₂₀H₂₆ClN₃O₅

CAS No.

24796-94-9

Appearance

Solid

Color

Light green to green

SMILES

CCN(C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)N=C2C=C1)CC.O=Cl(=O)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (117.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3591 mL	11.7955 mL	23.5910 mL
5 mM	0.4718 mL	2.3591 mL	4.7182 mL
10 mM	0.2359 mL	1.1796 mL	2.3591 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (251 KB)

Handling Instructions (2659 KB)

References

[1]. Gilani AG, et al. Estimation of ground- and excited-state dipole moments of oxazine 1 in liquid and liquid crystalline media. Spectrochim Acta A Mol Biomol Spectrosc. 2011 Jun;79(1):148-55. [Content Brief]

Kinase Assay
^[1]

Oxazine 1 perchlorate doped liquid crystal samples are prepared from a pair of quartz sheets sandwiched exactly at known gap and sealed. The introduction of the dissolved Oxazine 1 perchlorate in nematic solvents is achieved by capillary action. Oxazine 1 perchlorate concentrations are chosen to be 1×10⁻⁵ M for all the liquid samples. Oxazine 1 perchlorate is studied in the nematic media up to a concentration of about 0.1%, w/w. The absorption spectra of Oxazine 1 perchlorate are recorded on a double beam spectrophotometer over a wavelength range 300 to 800 nm combined with a cell temperature controller^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Gilani AG, et al. Estimation of ground- and excited-state dipole moments of oxazine 1 in liquid and liquid crystalline media. Spectrochim Acta A Mol Biomol Spectrosc. 2011 Jun;79(1):148-55. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3591 mL	11.7955 mL	23.5910 mL	58.9776 mL
	5 mM	0.4718 mL	2.3591 mL	4.7182 mL	11.7955 mL
	10 mM	0.2359 mL	1.1796 mL	2.3591 mL	5.8978 mL
	15 mM	0.1573 mL	0.7864 mL	1.5727 mL	3.9318 mL
	20 mM	0.1180 mL	0.5898 mL	1.1796 mL	2.9489 mL
	25 mM	0.0944 mL	0.4718 mL	0.9436 mL	2.3591 mL
	30 mM	0.0786 mL	0.3932 mL	0.7864 mL	1.9659 mL
	40 mM	0.0590 mL	0.2949 mL	0.5898 mL	1.4744 mL
	50 mM	0.0472 mL	0.2359 mL	0.4718 mL	1.1796 mL
	60 mM	0.0393 mL	0.1966 mL	0.3932 mL	0.9830 mL
	80 mM	0.0295 mL	0.1474 mL	0.2949 mL	0.7372 mL
	100 mM	0.0236 mL	0.1180 mL	0.2359 mL	0.5898 mL