Phalloidin

Cat. No.: HY-P0028 Purity: 99.83%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome .

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Phalloidin 화학구조

사이즈	재고	수량
500 μg	해외재고보유	Estimated Time of Arrival: December 31
1 mg	해외재고보유	Estimated Time of Arrival: December 31
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	견적 받기
50 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 23 publication(s) in Google Scholar

23 Publications Citing Use of MCE Phalloidin

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome ^[1].

In Vitro

Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

788.87

화학식

C₃₅H₄₈N₈O₁₁S

CAS No.

17466-45-4

Appearance

Solid

Color

White to off-white

Sequence

Ala-{d-Thr}-Cys-{Hyp}-Ala-Trp-Leu (Sulfide bridge: Cys3-Trp6)

Sequence Shortening

A-{d-Thr}-C-{Hyp}-AWL (Sulfide bridge: Cys3-Trp6)

선적

Room temperature in continental US; may vary elsewhere.

보관

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

용액&용해도

In Vitro:

DMSO : 100 mg/mL (126.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2676 mL	6.3382 mL	12.6764 mL
5 mM	0.2535 mL	1.2676 mL	2.5353 mL
10 mM	0.1268 mL	0.6338 mL	1.2676 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

순도&문서

Purity: 99.83%

Select Batch:

Data Sheet (270 KB) SDS (420 KB)

COA (242 KB) RP-HPLC (181 KB)

Handling Instructions (2659 KB)

References

[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947. [Content Brief]

Cell Assay ^[1]	Prepare a 1:200 dilution of the stock solution (300 units/mL) of fluorescein Phalloidin or rhodamine Phalloidin using PBS. Aspirate the cell medium from the cells grown on glass coverslips and rinse the cells three times with PBS. Fix the cells for 10 min in the 3.7% formaldehyde solution. Rinse the fixed cells three times for 5 min each in PBS. Permeabilize the cells for 5 min in the 0.2% Triton X-100 solution. Rinse the fixed cells three times with PBS. Label the cells with the fluorescein or rhodamine Phalloidin for 5 to 10 min at room temperature. Rinse the cells three times in PBS for 5 min each time. Mount the cell and view the cells using either the fluorescein or rhodamine filter set, depending on the probe^[1]. MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
References	[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947. [Content Brief]

Cell Assay
^[1]

Prepare a 1:200 dilution of the stock solution (300 units/mL) of fluorescein Phalloidin or rhodamine Phalloidin using PBS. Aspirate the cell medium from the cells grown on glass coverslips and rinse the cells three times with PBS. Fix the cells for 10 min in the 3.7% formaldehyde solution. Rinse the fixed cells three times for 5 min each in PBS. Permeabilize the cells for 5 min in the 0.2% Triton X-100 solution. Rinse the fixed cells three times with PBS. Label the cells with the fluorescein or rhodamine Phalloidin for 5 to 10 min at room temperature. Rinse the cells three times in PBS for 5 min each time. Mount the cell and view the cells using either the fluorescein or rhodamine filter set, depending on the probe^[1].

MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.

References

[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2676 mL	6.3382 mL	12.6764 mL	31.6909 mL
	5 mM	0.2535 mL	1.2676 mL	2.5353 mL	6.3382 mL
	10 mM	0.1268 mL	0.6338 mL	1.2676 mL	3.1691 mL
	15 mM	0.0845 mL	0.4225 mL	0.8451 mL	2.1127 mL
	20 mM	0.0634 mL	0.3169 mL	0.6338 mL	1.5845 mL
	25 mM	0.0507 mL	0.2535 mL	0.5071 mL	1.2676 mL
	30 mM	0.0423 mL	0.2113 mL	0.4225 mL	1.0564 mL
	40 mM	0.0317 mL	0.1585 mL	0.3169 mL	0.7923 mL
	50 mM	0.0254 mL	0.1268 mL	0.2535 mL	0.6338 mL
	60 mM	0.0211 mL	0.1056 mL	0.2113 mL	0.5282 mL
	80 mM	0.0158 mL	0.0792 mL	0.1585 mL	0.3961 mL
	100 mM	0.0127 mL	0.0634 mL	0.1268 mL	0.3169 mL

Phalloidin Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phalloidin17466-45-4Fluorescent DyeInhibitorinhibitorinhibit

최근 본 상품:

상품명

Requested Quantity *

고객명 *

호칭

메일주소 *

전화번호 *

Department

회사명 *

City

도

Country or Region *

비고

상품명

Lot #

고객명 *

메일주소 *

전화번호

Department *

회사명 *

Country or Region *

비고

Name
이메일 *

	Sorry, but the email address you supplied was invalid.

Phalloidin

고객리뷰

Phalloidin Related Antibodies

23 Publications Citing Use of MCE Phalloidin

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

순도&문서

References

고객리뷰

Phalloidin Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Phalloidin Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report