2. Metabolic Enzyme/Protease Neuronal Signaling Immunology/Inflammation Cell Cycle/DNA Damage Epigenetics
  3. UGT Cholinesterase (ChE) Tau Protein SOD Sirtuin
  4. Schisanhenol

Schisanhenol  (Synonyms: Schizanhenol; Gomisin-K3)

Cat. No.: HY-N0859 Purity: 99.99%
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Schisanhenol (Schizanhenol), a lignan, is an orally active antioxidant. Schisanhenol reduces AChE activity, increases SIRT1 and PGC-1α expression, and decreases phosphorylated Tau (Ser 396) levels. Schisanhenol increases SOD and glutathione peroxidase activity, decreases malondialdehyde (MDA) content, and inhibits UGT2B7 activitY. Schisanhenol attenuates ox-LDL-induced apoptosis, intracellular reactive oxygen species generation, and cytotoxicity in endothelial cells. Schisanhenol inhibits LDL oxidation, brain mitochondrial and membrane peroxidative damage, and brain mitochondrial swelling and disintegration. Schisanhenol can be used for the research of Alzheimer’s disease, atherosclerosis, brain ischemia, and age-related brain deterioration.

For research use only. We do not sell to patients.

Schisanhenol

Schisanhenol Chemical Structure

CAS No. : 69363-14-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Schisanhenol:

Schisanhenol Related Antibodies

Top Publications Citing Use of Products

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AChE BChE

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Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7 SIRT4

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Schisanhenol (Schizanhenol), a lignan, is an orally active antioxidant. Schisanhenol reduces AChE activity, increases SIRT1 and PGC-1α expression, and decreases phosphorylated Tau (Ser 396) levels. Schisanhenol increases SOD and glutathione peroxidase activity, decreases malondialdehyde (MDA) content, and inhibits UGT2B7 activitY. Schisanhenol attenuates ox-LDL-induced apoptosis, intracellular reactive oxygen species generation, and cytotoxicity in endothelial cells. Schisanhenol inhibits LDL oxidation, brain mitochondrial and membrane peroxidative damage, and brain mitochondrial swelling and disintegration. Schisanhenol can be used for the research of Alzheimer’s disease, atherosclerosis, brain ischemia, and age-related brain deterioration[1][2][3][4].

IC50 & Target

AChE

 

SIRT1

 

In Vitro

Schisanhenol attenuates ox-LDL-induced apoptosis and reactive oxygen species generation in cultured bovine aorta endothelial cells[1].
Schisanhenol scavenges oxygen radicals in cultured human neutrophils[1].
Schisanhenol protects cultured mouse spleen lymphocytes, isolated rat cerebral mitochondria and synaptosomes from oxygen free radical-induced injury[1].
Schisanhenol (10-50 μM; 1 h pre-incubation, then 24 h co-incubation with ox-LDL) protects bovine aorta endothelial cells against ox-LDL-induced cytotoxicity in a dose-dependent manner, with 50 μM producing the strongest effect by preserving cell morphology, increasing cell viability, and reducing LDH leakage[3].
Schisanhenol (10-50 μM; 24 h co-incubation with ox-LDL) inhibits ox-LDL-induced apoptosis in bovine aorta endothelial cells in a dose-dependent manner, with 10 μM producing a stronger reduction in sub-G1 apoptotic cells[3].
Schisanhenol (10-50 μM; 30 min pre-incubation with DCFH-DA, then 15 min stimulation with ox-LDL) inhibits ox-LDL-induced intracellular reactive oxygen species generation in bovine aorta endothelial cells, with 10 μM returning ROS levels to normal control values[3].
Schisanhenol (1-100 μM; 30 min) inhibits Fe2+-cysteine-induced lipid peroxidation and ATPase activity loss in 8-month-old rat brain membrane fractions in vitro in a dose-dependent manner[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Schisanhenol Related Antibodies

Cell Cytotoxicity Assay[3]

Cell Line: Bovine aorta endothelial cells (BAECs)
Concentration: 5-50 μM
Incubation Time: 1 h pre-incubation, then 24 h co-incubation with ox-LDL
Result: Increased cell survival.
Reduced LDH leakage.
Preserved normal BAEC morphology.
Did not significantly increase cell viability or reduce LDH leakage at 5 μM.

Apoptosis Analysis[3]

Cell Line: Bovine aorta endothelial cells (BAECs)
Concentration: 10 μM, 50 μM
Incubation Time: 24 h co-incubation with ox-LDL
Result: Reduced ox-LDL-induced chromatin condensation at 10 and 50 μM.
Diminished the intensity of typical internucleosomal DNA laddering at 10 and 50 μM.
Decreased the percentage of apoptotic cells in the sub-G1 phase to 33.9% at 50 μM.
Decreased the percentage of apoptotic cells in the sub-G1 phase to 23.8% at 10 μM.
In Vivo

Schisanhenol (10-100 mg/kg; i.p.; daily; 7 days) attenuates Scopolamine (HY-N0296)-induced cognitive impairment, reduces hippocampal AChE activity and oxidative damage, and modulates the SIRT1-PGC-1α-Tau signaling pathway in male Kunming mice[1].
Schisanhenol (150 mg/kg; p.o.) significantly increases brain cytosol glutathione-peroxidase activity in mice subjected to brain ischemia-reperfusion, with a measured activity of 0.898 nmol/mg/5 min[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Kunming mice (male, 20-30 g) injected with Scopolamine[1]
Dosage: 10 mg/kg; 30 mg/kg; 100 mg/kg
Administration: i.p.; daily; 7 days
Result: Significantly reduced escape latency in the Morris water maze.
Significantly increased time spent in the target quadrant during the probe test.
Significantly reduced acetylcholinesterase (AChE) and malondialdehyde (MDA) activity in hippocampal tissue.
Significantly increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) activity in hippocampal tissue.
Significantly increased SIRT1 and PGC-1α protein levels in hippocampal tissue.
Significantly reduced phosphorylated Tau (Ser396) protein levels in hippocampal tissue.
Molecular Weight

402.48

Formula

C23H30O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

COC(C(OC)=C(OC)C=C1C[C@H](C)[C@H](C)C2)=C1C3=C2C=C(OC)C(OC)=C3O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (621.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4846 mL 12.4230 mL 24.8460 mL
5 mM 0.4969 mL 2.4846 mL 4.9692 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 6.25 mg/mL (15.53 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 6.25 mg/mL (15.53 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4846 mL 12.4230 mL 24.8460 mL 62.1149 mL
5 mM 0.4969 mL 2.4846 mL 4.9692 mL 12.4230 mL
10 mM 0.2485 mL 1.2423 mL 2.4846 mL 6.2115 mL
15 mM 0.1656 mL 0.8282 mL 1.6564 mL 4.1410 mL
20 mM 0.1242 mL 0.6211 mL 1.2423 mL 3.1057 mL
25 mM 0.0994 mL 0.4969 mL 0.9938 mL 2.4846 mL
30 mM 0.0828 mL 0.4141 mL 0.8282 mL 2.0705 mL
40 mM 0.0621 mL 0.3106 mL 0.6211 mL 1.5529 mL
50 mM 0.0497 mL 0.2485 mL 0.4969 mL 1.2423 mL
60 mM 0.0414 mL 0.2070 mL 0.4141 mL 1.0352 mL
80 mM 0.0311 mL 0.1553 mL 0.3106 mL 0.7764 mL
100 mM 0.0248 mL 0.1242 mL 0.2485 mL 0.6211 mL
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Schisanhenol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Schisanhenol69363-14-0Schizanhenol Gomisin-K3UGTCholinesterase (ChE)Tau ProteinSODSirtuinUDP-glucuronosyltransferaseUridine diphosphate glucuronosyltransferaseSuperoxide DismutaseUDP-glucuronosyltransferase 2B7superoxide dismutaseendothelial cellsphosphorylated Tau (Ser 396)SIRT1acetylcholinesterasePGC-1αbovine aorta endothelial cellsAlzheimer’s diseaseglutathione peroxidaseInhibitorinhibitorinhibit

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