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  4. WST-8

WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

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WST-8

WST-8 構造式

CAS 番号 : 193149-74-5

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 72 在庫あり
Solution
10 mM * 1 mL in Water USD 72 在庫あり
Solid
25 mg $66 在庫あり
50 mg $106 在庫あり
100 mg $158 在庫あり
200 mg $264 在庫あり
500 mg $490 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 16 publication(s) in Google Scholar

WST-8 関連抗体

Top Publications Citing Use of Products

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

  • カスタマーレビュー

製品説明

WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

体外実験

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation
1.1 Preparation of stock solution
Solvent: H2O
Concentration: 50 mM is recommended.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 5 mM with cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Cells are seeded in a 96-well plate at a concentration of 1.0-2.0?×?103 cells/well/100μL
2.2 Add 10 μL WST-8 at a final concentration of 5 mM.
2.3 Incubate cells at room temperature or 37°C for 1-4?h.
2.4 The absorbance at 450?nm was determined with a microplate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

WST-8 関連抗体
分子量

600.47

分子式

C20H13N6NaO11S2
CAS 番号
Appearance

Solid

Color

White to yellow

SMILES

COC1=CC([N+]([O-])=O)=CC=C1[N+]2=NC(C3=CC=C(C=C3S(=O)([O-])=O)S(=O)(O[Na])=O)=NN2C4=CC=C(C=C4)[N+]([O-])=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
体外: 

H2O : 80 mg/mL (133.23 mM; ultrasonic and warming and heat to 60°C)

DMSO : 10 mg/mL (16.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6654 mL 8.3268 mL 16.6536 mL
5 mM 0.3331 mL 1.6654 mL 3.3307 mL
10 mM 0.1665 mL 0.8327 mL 1.6654 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション

純度: 99.69%

COA (258 KB) HNMR (146 KB) RP-HPLC (168 KB) LCMS (234 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 1.6654 mL 8.3268 mL 16.6536 mL 41.6341 mL
5 mM 0.3331 mL 1.6654 mL 3.3307 mL 8.3268 mL
10 mM 0.1665 mL 0.8327 mL 1.6654 mL 4.1634 mL
15 mM 0.1110 mL 0.5551 mL 1.1102 mL 2.7756 mL
H2O 20 mM 0.0833 mL 0.4163 mL 0.8327 mL 2.0817 mL
25 mM 0.0666 mL 0.3331 mL 0.6661 mL 1.6654 mL
30 mM 0.0555 mL 0.2776 mL 0.5551 mL 1.3878 mL
40 mM 0.0416 mL 0.2082 mL 0.4163 mL 1.0409 mL
50 mM 0.0333 mL 0.1665 mL 0.3331 mL 0.8327 mL
60 mM 0.0278 mL 0.1388 mL 0.2776 mL 0.6939 mL
80 mM 0.0208 mL 0.1041 mL 0.2082 mL 0.5204 mL
100 mM 0.0167 mL 0.0833 mL 0.1665 mL 0.4163 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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WST-8 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WST-8193149-74-5WST8WST 8Fluorescent DyeInhibitorinhibitorinhibit

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製品名:
WST-8
製品番号:
HY-D0831
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