AF 647 carboxylic acid 

AF 647 carboxylic acid is a derivative of the far-infrared dye AF 647. AF 647 is often used as a replacement for Cy5 dye, with an excitation wavelength (λ_ex) of 635 nm (conventional fluorescence detection)/620 nm (instantaneous detection). The maximum excitation wavelength is 647 nm and the maximum emission wavelength is 671 nm. AF 647 carboxylic acid has a carboxylic acid group and can be coupled to biomolecules with amino groups to achieve dye labeling^[1].

959.26

C₃₅H₄₁K₃N₂O₁₄S₄

Solid

Brown to dark brown

CC(C1=CC(S(=O)(O[K])=O)=CC=C1N/2CCCS(=O)(O[K])=O)(C)C2=C/C=C/C=C/C3=[N+](CCCS(=O)([O-])=O)C4=CC=C(S(=O)(O[K])=O)C=C4C3(C)CCCCC(O)=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Karlsson J K G, Laude A, Hall M J, et al. Photo‐isomerization of the Cyanine Dye Alexa‐Fluor 647 (AF‐647) in the Context of dSTORM Super‐Resolution Microscopy[J]. Chemistry–A European Journal, 2019, 25(65): 14983-14998.