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Alkyne tyramide 

Cat. No.: HY-131442
Handling Instructions

Alkyne tyramide is a clickable ascorbate peroxidase 2 (APEX2) probe. Alkyne tyramide substantially improves APEX-labeling efficiency in intact yeast cells, as it is more cell wall-permeant than APEX2 substrate biotin-phenol (BP). Alkyne tyramide also facilitates the identification of APEX-labeling sites, allowing the unambiguous assignment of membrane topology of mitochondrial proteins.

For research use only. We do not sell to patients.

Alkyne tyramide Chemical Structure

Alkyne tyramide Chemical Structure

CAS No. : 1694495-59-4

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Description

Alkyne tyramide is a clickable ascorbate peroxidase 2 (APEX2) probe. Alkyne tyramide substantially improves APEX-labeling efficiency in intact yeast cells, as it is more cell wall-permeant than APEX2 substrate biotin-phenol (BP). Alkyne tyramide also facilitates the identification of APEX-labeling sites, allowing the unambiguous assignment of membrane topology of mitochondrial proteins[1].

In Vitro

Yeast cells expressing Su9-APEX2 were incubated with each probe at 2.5 mM final concentration for half an hour, and the labeling reaction was initiated by the addition of 1 mM H2O2. Alkyne tyramide is the most reactive APEX2 substrate for labeling the yeast proteome[1].
APEX2-mediated Alkyne tyramide labeling in the yeast mitochondria is more effective at probe concentrations above 1 mM[1].
Alkyne tyramide enables proteomic profiling in the mitochondrial matrix of intact yeast cells with exceptionally high specificity (94%), and offers higher coverage than the traditional APEX2 substrate biotin-phenol (BP)[1].

Molecular Weight

217.26

Formula

C₁₃H₁₅NO₂

CAS No.

1694495-59-4

SMILES

C#CCCC(NCCC1=CC=C(O)C=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

Alkyne tyramideOthersyeastmitochondriaAPEX2substratemembranetopologyInhibitorinhibitorinhibit

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Alkyne tyramide
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