CD154 Antibody (YA9221)

Cat. No.: HY-P89877: Data Sheet User Guide for Antibodies
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CD154 Antibody (YA9221) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD154.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD154 Antibody (YA9221) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD154.

Host

Mouse

Molecular Weight

Predicted band size: 29 kDa

Species Reactivity

human

SwissProt ID

P29965

Gene ID

959 [NCBI]

Immunogen

A synthesized peptide derived from human CD154.

Application &
Dilution Ratio

Application	Dilution Ratio
IP IP: Immunoprecipitation	1-2μg per 100-500μg Total protein
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-500
FC FC: Flow Cytometry	1μg per 1*10⁶ cells

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

Storage & Stability

Stored at 2-8°C for 1 year, do not freeze.

Shipping

Shipping with blue ice.

Background

Function：Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity); Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling

Subcellular Localization：Cell membrane; Single-pass type II membrane protein; Cell surface; Secreted

Expression：
Tissue_specificity:Specifically expressed on activated CD4+ T-lymphocytes

Subunit：Homotrimer (PubMed:11676606, PubMed:8589998, PubMed:8626375). Interacts with isoform 3 of CD28 (PubMed:15067037). CD40 ligand, soluble form: Exists as either a monomer or a homotrimer (PubMed:31331973, PubMed:8626375). Forms a ternary complex between CD40 and integrins for CD40-CD40LG signaling (PubMed:31331973)

Database

OMIM: 308230

Synonyms

CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5

Documentation

User Guide for Antibodies (1077 KB)