Cytokeratin 14 Antibody (YA6355)

Cat. No.: HY-P86663: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Cytokeratin 14 Antibody (YA6355) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 14.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Cytokeratin 14 Antibody (YA6355) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 14.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 52 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P02533

Gene ID

3861 [NCBI]

Immunogen

Recombinant human Cytokeratin 14 protein, around C-terminal 150aa

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Expressed in the corneal epithelium (protein level) (PubMed:26758872) . Detectable in the basal layer, with decreased expression in higher layers, particularly the spinous and granular layers, but not detected in the stratum corneum. Strongly expressed in the outer root sheath of hair follicles during the anagen phase, but not in the germinal stroma, inner root sheath, or hair itself (PubMed:9457912) . Found in keratinocytes surrounding the hair bulb during the telogen phase (PubMed:9457912) .

Subunit：Forms a disulfide-linked heterodimer (via 2B domains) with KRT5 (via 2B domains) (PubMed:22705788, PubMed:24940650).

Synonyms

Cytokeratin 14; CK 14; Cytokeratin14; Cytokeratin-14; Dowling Meara; ebs3; Epidermolysis bullosa simplex; k14; Keratin 14; Keratin type I cytoskeletal 14; Keratin14; Koebner; Krt 14; krt14; EBS3; EBS4; NFJ; Keratin, type I cytoskeletal 14; K1C14_HUMAN.

Documentation

User Guide for Antibodies (1077 KB)