DMT1 Antibody (YA4629)

製品番号: HY-P84933: データシート SDS COA User Guide for Antibodies
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DMT1 Antibody (YA4629) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DMT1.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$72	在庫あり	Estimated Time of Arrival: December 31
50 μL	$185	在庫あり	Estimated Time of Arrival: December 31
100 μL	$300	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

DMT1 Antibody (YA4629) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DMT1.

Host

Rabbit

Clonality

Monoclonal,Recombinant

分子量

Predicted band size: 62 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P49281

Gene ID

4891 [NCBI]

Immunogen

Recombinant protein within human DMT1 aa 1-400.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
FC FC: Flow Cytometry	1:1000

純度	Affinity Purified	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HEK293 (lane 2(20μg), HepG2 (lane 3(20μg), Hela (lane 4(20μg) using DMT1 (HY-P84933) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Proton-coupled metal ion symporter operating with a proton to metal ion stoicH2O2etry of 1:1 (PubMed:17109629, PubMed:17293870, PubMed:22736759, PubMed:25326704, PubMed:25491917). Selectively transports various divalent metal cations, in decreasing affinity: Cd(2+) > Fe(2+) > Co(2+), Mn(2+) >> Zn(2+), Ni(2+), VO(2+) (PubMed:17109629, PubMed:17293870, PubMed:22736759, PubMed:25326704, PubMed:25491917). Essential for maintenance of iron homeostasis by modulating intestinal absorption of dietary Fe(2+) and TF-associated endosomal Fe(2+) transport in erytH2O2d precursors and other cells (By similarity). Enables Fe(2+) and Mn(2+) ion entry into mitochondria, and is thus expected to promote mitochondrial heme synthesis, iron-sulfur cluster biogenesis and antioxidant defense (By similarity) (PubMed:24448823). Can mediate uncoupled fluxes of either protons or metal ions

Subcellular Localization：Early endosome membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein; Late endosome membrane; Multi-pass membrane protein; Lysosome membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Extracellular vesicle membrane; Multi-pass membrane protein; Cell membrane; Mitochondrion outer membrane; Multi-pass membrane protein; Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein; Recycling endosome membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Ubiquitously expressed. Expressed in erytH2O2d progenitors

Induction:Up-regulated under iron-depletion conditions. Down-regulated in response to high extracellular iron levels; Up-regulated under iron-depletion conditions. Down-regulated in response to high extracellular iron levels; Up-regulated under iron-depletion conditions. Down-regulated in response to high extracellular iron levels

Isoforms & Post-Translational Modification：P49281 has 5 isomers: P49281-1: 62266 Da (predicted); P49281-2: 61456 Da (predicted); P49281-3: 64704 Da (predicted); P49281-4: 65514 Da (predicted); P49281-5: 61048 Da (predicted).
Ubiquitinated by WWP2;N-glycosylated

Subunit：Forms a complex with NDFIP1 and NEDD4L, in cortical neurons, in response to iron and cobalt exposure; this interaction leads to SLC11A2 ubiquitination by NEDD4L and proteasome-dependent degradation (PubMed:19706893).

RRID

AB_3719007

Database

Entrez Gene: 4891 Human

SwissProt: P49281 Human

OMIM: 600523 Human

Synonyms

DCT 1 antibody; dct-1 antibody; DCT1 antibody; Divalent cation transporter 1 antibody; Divalent metal transporter 1 antibody; DMT 1 antibody; DMT-1 antibody; DMT1 antibody; FLJ37416 antibody; Natural resistance associated macrophage protein 2 antibody; Natural resistance-associated macrophage protein 2 antibody; NRAM2_HUMAN antibody; NRAMP 2 antibody; NRAMP2 antibody; OK/SW-cl.20 antibody; Slc11a2 antibody; Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2 antibody; Solute carrier family 11 member 2 antibody

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/hela-cell.html [Content Brief]