MUC2 Antibody (YA5674)
(Synonyms: SMUC, MUC2, Mucin-2, MUC-2, Intestinal mucin-2)
Based on 1 Customer Validation
MUC2 Antibody (YA5674) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MUC2.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P, ICC/IF, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-400 | 1:50-200 | 1:500-5000 |
Product Details
MUC2 Antibody (YA5674) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MUC2.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
Synthesized peptide derived from human MUC2 AA range: 1800-2000
affinity chromatography.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Immunohistochemical analysis of paraffin-embedded human colon cancer using MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85982, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human prostate cancer using MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85982, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human cervical cancer using MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85982, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon using MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85982, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney using MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85982, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast cancer using MUC2antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85982, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver using MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85982, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
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Function
MUC2 encodes the major gel-forming mucin produced by intestinal goblet cells and constitutes the structural backbone of the gastrointestinal mucus barrier, where it physically separates luminal microorganisms from the epithelial surface and supports mucosal homeostasis[4][5]. Mechanistically, MUC2 polymers form highly organized mucus networks that generate the characteristic inner and outer colonic mucus layers, thereby regulating microbial access, antigen exposure, and host defense responses[1][2]. Through these barrier functions, MUC2 contributes to intestinal immune regulation and maintenance of a stable microbiota, while defective synthesis, secretion, or organization of MUC2 compromises barrier integrity and promotes epithelial contact with bacteria[5][6]. In disease settings, dysregulated MUC2 production has been closely associated with inflammatory bowel disease and chronic intestinal inflammation, and experimental studies demonstrate that impairment of the MUC2 barrier increases susceptibility to colitis and inflammation-related pathology[2][6][3]. Compared with related membrane-associated mucins such as MUC1, MUC3, and MUC17, MUC2 is distinguished by its role as a secreted polymeric mucin that forms the bulk extracellular mucus scaffold rather than functioning primarily at the epithelial cell surface[3]. For experimental applications, MUC2 serves as a widely used biomarker of goblet cell differentiation, mucus barrier integrity, and host-microbiota interactions, making it a valuable target for studies investigating intestinal barrier dysfunction, mucosal immunity, and therapeutic strategies aimed at restoring mucus homeostasis[4][6].
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Subcellular Localization
Secreted
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Expression
Tissue_specificity:Colon, small intestine, colon tumor, bronchi, cervix, and gallbladder -
Subunit
Homomultimer; disulfide-linked (PubMed:12374796, PubMed:31310764, PubMed:33031746, PubMed:35377815). The N- and C-terminus mediate their assembly into higher order structures to form filaments (PubMed:33031746, PubMed:35377815). The CTCK domains of two polypeptides associate in the endoplasmic reticulum to generate intermolecularly disulfide-bonded dimers (By similarity). These dimers progress to the Golgi apparatus, which is a more acidic environment than the endoplasmic reticulum (PubMed:33031746). Under acidic conditions, the N-termini form non-covalent intermolecular interactions that juxtapose assemblies of the third VWD domain (VWD3) from different CTCK-linked dimers (PubMed:33031746). The VWD3 assemblies then become disulfide bonded to one another to produce long, disulfide-linked polymers that remain highly compact until secretion (PubMed:33031746). Interacts with FCGBP (PubMed:19432394). Interacts with AGR2; disulfide-linked (PubMed:19359471)
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SwissProt ID
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Synonyms
SMUC, MUC2, Mucin-2, MUC-2, Intestinal mucin-2
Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[3]. Liu Y, et al. Orchestration of MUC2 - The key regulatory target of gut barrier and homeostasis: A review. Int J Biol Macromol. 2023 May 1;236:123862. [Content Brief]
[4]. Kim YS, et al. Intestinal goblet cells and mucins in health and disease: recent insights and progress. Curr Gastroenterol Rep. 2010;12(5):319-330. [Content Brief]
[5]. Johansson ME, et al. The two mucus layers of colon are organized by the MUC2 mucin, whereas the outer layer is a legislator of host-microbial interactions. Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1(Suppl 1):4659-65. [Content Brief]
[6]. Yao D, et al. MUC2 and related bacterial factors: therapeutic targets for ulcerative colitis. EBioMedicine. 2021;74:103751. [Content Brief]
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