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MuRF1 Antibody (YA4416)

(Synonyms: TRIM63; IRF; SMRZ; MURF2; RNF28)
Customer Review

Based on 1 Customer Validation

MuRF1 Antibody (YA4416) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MuRF1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL IHC-P FC ICC
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • MuRF1 Antibody (YA4416) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

MuRF1 Antibody (YA4416) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MuRF1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 40-45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40.2 kDa
Immunogen

Synthesized peptide of human MuRF1 (AA: 293-304).

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718969

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    Immunohistochemical analysis of paraffin-embedded human heart tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    Flow cytometric analysis of 1X10^6 Hela cells labeling MuRF1 Antibody(HY-P84719
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    Immunofluorescence analysis of Hela cells labeling MuRF1 antibody (HY-P84719) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

Background

  • Function

    MuRF1 is an E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells

  • Subcellular Localization

    Cytoplasm; Nucleus; Cytoplasm, myofibril, sarcomere, M line; Cytoplasm, myofibril, sarcomere, Z line

  • Expression


    Tissue_specificity:Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris

  • Subunit

    Homodimer. Homooligomer and heterooligomer. Interacts with SUMO2, titin/TTN and GMEB1. Interacts with TRIM54 and probably with TRIM55 and TNNI3. Forms a ternary complex with RACK1 and PRKCE (By similarity). Interacts with CKM

  • SwissProt ID

    Q969Q1

  • Gene ID
    84676 [NCBI]
  • Synonyms

    TRIM63; IRF; SMRZ; MURF2; RNF28

MuRF1 Antibody (YA4416) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling MuRF1 Antibody(HY-P84719

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling MuRF1 antibody (HY-P84719) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • MuRF1 Antibody (YA4416) Product Image
    1/16
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • MuRF1 Antibody (YA4416) Product Image
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling MuRF1 Antibody(HY-P84719
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling MuRF1 antibody (HY-P84719) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • Experimental Validation Results for MuRF1 Antibody (YA4416)
  • MuRF1 Antibody (YA4416) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MuRF1 Antibody (YA4416) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MuRF1 Antibody (YA4416) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MuRF1 Antibody (YA4416) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MuRF1 Antibody (YA4416) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MuRF1 Antibody (YA4416) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using MuRF1 Antibody (YA4416). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84719,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MuRF1 Antibody (YA4416) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling MuRF1 Antibody(HY-P84719

  • MuRF1 Antibody (YA4416) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling MuRF1 antibody (HY-P84719) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • MuRF1 Antibody (YA4416) Product Image IHC-P
  • MuRF1 Antibody (YA4416) Product Image IHC-P
  • MuRF1 Antibody (YA4416) Product Image IHC-P
  • MuRF1 Antibody (YA4416) Product Image IHC-P
  • MuRF1 Antibody (YA4416) Product Image IHC-P
  • MuRF1 Antibody (YA4416) Product Image IHC-P
  • MuRF1 Antibody (YA4416) Product Image FC
  • MuRF1 Antibody (YA4416) Product Image ICC